硫酸盐还原菌对EH40焊接钢海水腐蚀的影响

目的研究无菌和SRB体系中EH40钢焊接接头的母材区、热影响区和焊缝区腐蚀差异。方法利用金相显微镜观察、失重法、电化学测试、扫描电镜和X射线光电子能谱仪等分析手段。结果焊缝区微观组织由块状铁素体(BLF)和魏氏组织(W)组成;热影响区可再分为粗晶区(CGHAZ)和细晶区(FGHAZ)两个区;母材区主要由BLF、AF和少量珠光体组成。在无菌对照体系中,焊缝区腐蚀速率为0.0026 mm/a,混合区次之,为0.0023 mm/a,母材区最小,为0.0019 mm/a。含SRB体系中,各区的腐蚀速率为:焊缝区(0.0037 mm/a)混合区(0.0034 mm/a)母材区(0.0030 mm/a)。热影响区腐蚀产物形貌粗大,点蚀孔也较大。结论焊缝区比母材区容易腐蚀,由此易造成小阳极大阴极加速腐蚀,热影响区容易发生点蚀。SRB能促进三个区域试样腐蚀加速。     

Characterization of microbial communities from coastal waters using microarrays

Molecular methods, including DNA probes, were used to identify and enumerate pathogenic Vibrio species in the Chesapeake Bay; our data indicated that Vibrio vulnificus exhibits seasonal fluctuations in number. Our work included a characterization of total microbial communities from the Bay; development of microarrays that identify and quantify the diversity of those communities; and observation of temporal changes in those communities. To identify members of the microbial community, we amplified the 16S rDNA gene from community DNA isolated from a biofilm sample collected from the Chesapeake Bay in February, 2000. The resultant 75 sequences were 95% or more similar to 7 species including two recently described Shewanella species, baltica and frigidimarina, that have not been previously isolated from the Chesapeake. When the genera of bacteria from biofilm after culturing are compared to those detected by subcloning amplified 16S fragments from community DNA, the cultured sample exhibited a strong bias. In oysters collected in February, the most common bacteria were previously unknown. Based on our 16S findings, we are developing microarrays to detect these and other microbial species in these estuarine communities. The microarrays will detect each species using four distinct loci, with the multiple loci serving as an internal control. The accuracy of the microarray will be measured using sentinel species such as Aeromonas species, Escherichia coli, and Vibrio vulnificus. Using microarrays, it should be possible to determine the annual fluctuations of bacterial species (culturable and non-culturable, pathogenic and non-pathogenic). The data may be applied to understanding patterns of environmental change; assessing the health of the Bay; and evaluating the risk of human illness associated with exposure to and ingestion of water and shellfish.     

硫酸厂淤泥中SRB的选育及特性研究

从硫酸厂废水淤泥中分离筛选出了一株 SRB,其菌体形态呈弧状或细杆状 ,成熟细胞大小 0 .4～ 0 .6× 3 .0～ 5 .0μm,摇摆式运动 ,单极生鞭毛 ,芽孢染色阴性 ,革兰氏染色阴性 ;最适生长 p H6.0～ 6,5 ;适量氧气不会影响细菌生长 ,菌体内含过氧化氢酶。本文还探讨了该类细菌的分离培养方法 ,考察了分离菌部分生理生化特征及对硫酸盐还原能力 ,优化了培养基配方 ,并初步鉴定分离菌为脱硫弧菌     

利用光合细菌处理养猪场污水的试验研究

采用煤渣作为填料 ,光合细菌培养物作为挂膜菌的生物接触氧化法 ,对养猪场污水处理进行了试验研究 ,结果表明 :CODCr,NH3 N ,NO-2 N ,S2 - 的平均去除率分别达到 89 3% ,80 1 % ,90 6% ,82 4% ;水中光合细菌数从580个 /mL增加到 4 8× 1 0 4 个 /mL ,达到了废水资源化的目的。     

Acid resistance of methanogenic bacteria in a two-stage anaerobic process treating high concentration methanol Wastewater

In this study, the two-stage upflow anaerobic sludge blanket (UASB) system and batch experiments were employed to evaluate the performance of anaerobic digestion for the treatment of high concentration methanol wastewater. The acid resistance of granular sludge and methanogenic bacteria and their metabolizing activity were investigated. The results show that the pH of the first UASB changed from 4.9 to 5.8 and 5.5 to 6.2 for the second reactor. Apparently, these were not the advisable pH levels that common methanogenic bacteria could accept. The methanogenic bacteria of the system, viz. Methanosarcina barkeri, had some acid resistance and could still degrade methanol at pH 5.0. If the methanogenic bacteria were trained further, their acid resistance would be improved somewhat. Granular sludge of the system could protect the methanogenic bacteria within its body against the impact of the acidic environment and make them degrade methanol at pH 4.5. The performance of granular sludge was attributed to its structure, bacteria species, and the distribution of bacterium inside the granule. Translated from Acta Scientiae Circumstantiae, 2004, 24(4): 633–636 [译自: 环境科学学报]     

太湖水中降解苯酚细菌的分布与降解能力研究

太湖水中降解苯酚的细菌有3个属，分别是假单胞菌属、微球菌属和芽孢杆菌属。芽孢杆菌属和假单胞菌属是降解苯酚的主要细菌。未驯化细菌对1mg/L苯酚的降解率介于70％～85％。驯化细菌对苯酚的降解能力有了很大提高，对60mg／L苯酚的降解率达到了60％，86％。CS1培养基适宜测试未驯化细菌对苯酚的降解能力，测试驯化细菌则要用CS2培养基。     

活性污泥生长的控制

在污水处理过程中，针对不同的运行环境，我们选择适当的运行工艺和参数。通过控制菌胶团细菌和丝状菌平衡生长，或者是使固着型原生动物占优势，来获得良好的活性污泥，保证出水达标排放。     

草甸棕壤中镉、铅、油含量对微生物类群和生化活性的影响

本研究以重金属化合物(CdCl_2、PbCl_2)和矿物油作为土壤的污染物,分析土壤中镉、铅、油的含量对土壤微生物类群与生化活性的影响。结果表明,镉、铅、油对水旱田中的细菌抑制作用比较明显,对放线菌、真菌抑制作用较差。小剂量的镉、铅、油对固氮菌有刺激作用,大剂量则有抑制作用。对尿酶和硝化细菌的代谢有明显抑制作用,对其它酶类不明显。 从含重金属的平板培养基上反映出土壤对重金属的掩蔽作用。污染物对微生物的生化活性的临界毒害浓度分别为:镉 5—60ppm;铅 300—500ppm;油 500—5000ppm。     

工程菌处理高纯二甲醚生产废水的研究

工程菌LEY7是以假单胞菌D3 菌株 (Pseudomonassp.D3 )为受体 ,以缓慢牙孢杆菌 3RM2 菌株 (Bacilluslentus 3RM2 ) ,假单胞菌W1 (Pseudomonassp .W1 )和W2 (Pseudomonassp .W2 )为供体 ,用多基因转化原生质球构建而成。工程菌LEY7能同时降解 4种化合物。用工程菌LEY7处理化工废水 ,工艺采用二级氧化。处理效果 :进水COD =10 0 0mg/L ,处理后出水COD在 10 0mg/L以下。COD去除率达到 90 %以上。