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581.
Pierre Canetto 《International journal of occupational safety and ergonomics》2013,19(2):141-153
Occupational noise specialists do not generally recommend hearing protection devices (HPDs) as a preferred solution to noise risk prevention. Nevertheless, these devices are widely used and are in fact often necessary. Selection of an HPD should take into account comfort and the capacity for perceiving external signals, when they are worn. Current European regulations require that HPD attenuation be considered, when comparing noise exposure to limit values. However, HPD attenuation is effectively unknown under real-world conditions. Some methods are designed to give approximate attenuation values and these provide results within a wide statistical range. Field measurement methods and current standards have been developed to deal with this situation. The specific characteristic of impulse noise requires establishment of dedicated criteria and tools for HPD selection and testing. This paper introduces a number of avenues for research, which could be of assistance in improving HPD selection, qualification and design. 相似文献
582.
Yin-Jou Chou Chia-Yu Ou Te-Yao Hsu Chia-Wei Liou Cheng-Feng Lee Dan-Ju Tso Yau-Huei Wei 《黑龙江环境通报》2004,24(5):367-370
We prenatally diagnosed MELAS syndrome in a fetus whose mother and older brother had the MELAS-specific A3243G mutation. The mutant mtDNA level of the amniotic fluid cells was not significantly different from that of the postnatal peripheral blood and hair follicle samples. The obstetrical course was uncomplicated except for transient exacerbation of the mother's diabetes, which required insulin control. At term, the infant was macrosomic, and the delivery was complicated by shoulder dystocia. MELAS syndrome in itself does not influence either the prenatal course of the mother or the fetal outcome. In contrast to the fulminating clinical course of this mother's first child, MELAS symptoms did not develop in her second child until age four, despite similar high tissue levels of mutant mtDNA. The phenotypic diversity in two offspring with similar higher levels of mutant mtDNA suggests that prenatal genetic diagnosis of cultured amniotic cells may yield results that are poor prognosticators of fetal outcome. Copyright © 2004 John Wiley & Sons, Ltd. 相似文献
583.
李文 《中国人口.资源与环境》2002,12(1):27-31
本文将分析中国的人力资源条件对劳动生产率和科技创新能力的制约以及在国际性的人才争夺中所处的被动地位 ,提出发展教育和科技、更新观念和体制 ,是提升综合国力、实现可持续发展的重要途径。 相似文献
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采用PCR方法扩增了ZNF313基因的启动子序列,构建了含人ZNF313基因启动子不同片断的荧光素酶报告基因表达体系.以pRLTK为内参照质粒,瞬时转染HEK293T细胞,48h后收集细胞,测定荧光素酶的相对表达活性.结果发现,在ZNF313基因的启动子区域构建了4种荧光素酶报告基因表达体系,即pGL3215(-215bp~ 38bp)、pGL3160(-160bp~ 38bp)、pGL3133(-133bp~ 128bp)和pGL38(-8bp~ 128bp).其中pGL3215表达载体的荧光素酶相对表达活性最高;pGL3160和pGL3133表达载体的荧光素酶相对表达活性几乎相同,且是pGL3215的75%;而pGL38的荧光素酶相对表达活性急剧下降,接近于零.这表明,-133bp~-8bp区域内含有人ZNF313基因转录所必需的启动子序列.生物信息学的分析表明,两个SP1、一个AP2和一个TAg是人ZNF313基因启动子所必需的.图4参19 相似文献
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David Chitayat Robert W. Marion Linda Squillante Dagmar K. Kalousek Kiron M. Das MD Ph.D. 《黑龙江环境通报》1990,10(11):725-732
Since its introduction, prenatal diagnosis of chromosomal and metabolic disorder by mid-trimester amniocentesis has relied upon the use of a mixture of fetal cells obtained from amniotic fluid. Little knowledge has been gained in the sorting of these cells for diagnosis of tissue-specific disorders. In an attempt to determine the contribution of fetal colonic mucosal cells to the overall amniocyte population, we used the colonic epithelial-specific monoclonal antibody (MC-Ab) 7E12H12, IgM isotype. Specimens of the small intestine, colon, buccal mucosa, kidney, urinary bladder, and umbilical cord were obtained from electively aborted normal fetuses of 12–28 weeks' gestation. All of these specimens were examined with 7E12H12 by the immunoperoxidase technique. The MC-Ab reacted with the colonic epithelial cells but not with any of the other tissues. In addition, 40 amniotic fluid samples obtained from women between 16 and 18 weeks of gestation, who underwent amniocentesis because of advanced maternal age, were tested using a fluorescent activated cell sorter. Among the amniotic fluid specimens examined, 18·4 ± 10·3 percent cells reacted with 7E12H12. Double immunofluorescence studies revealed that all Mc-Ab-stained cells contained secretory component, confirming that they were epithelial in origin. All fetuses whose amniotic fluid was analysed had normal karyotypes and amniotic fluid alpha-fetoprctein levels that were also normal. This study demonstrates that cell-specific Mc-Ab can be used to detect colon cells in the amniotic fluid and that colon cells contribute significant numbers in the mixture of amniotic fluid cells. This technique could be helpful in the prenatal diagnosis of disorders in which the flow of amniotic fluid through the fetal intestine is impaired, such as cystic fibrosis, imperforate anus, Hirschsprung aganglionic megacolon, and intestinal atresia. 相似文献
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Cytogenetic parameters (the mitotic rate and the frequency of pathological mitoses) of the seed offspring of weeping birch (Betula pendula Roth.) and the frequency of micronuclei in the buccal epithelium of children living in several raions (districts) of the city of Voronezh differing in environmental conditions were studied. Judging from these parameters, the environmental situation was the worst in the Sovetskii raion. A correlation was found between the frequency of pathological mitoses in the roots of birch seedlings and the frequency of micronuclei in the buccal epithelial cells of children. This indicates that the cytogenetic monitoring data obtained in plants may be extrapolated to man. 相似文献