Physicochemical substance properties as indicators for unreliable exposure in microplate-based bioassays

In the last years many efforts were made to transform standardized algal test protocols into low-cost microplate assays. While advantages were pointed out frequently, limitations are not systematically addressed, thus hindering a widespread utilisation. In this study a group of organic substances with a wide distribution of volatility (log K_AW from −6.53 to −2.13) and lipophilicity (log K_OW from 1.26 to 4.92) was investigated with respect to the influence of these physicochemical properties on their algal toxicity in different assays. Therefore the EC₅₀ values were determined with a microplate assay based on ISO 8692 protocol and the results were compared with those of an established algal growth inhibition test conducted in air tight glass vessels. Using the ratio of the EC₅₀ values, a clear connection between biological response and volatility as well as lipophilicity of test substances could be detected. Chemicals with a log K_OW higher than 3 or a Henry coefficient log K_AW higher than −4 were identified as less effective in the microplate assay than in the comparative assay. The loss in nominal concentration due to physicochemical properties could be shown to contribute to this using HPLC analysis. Consequently, when using microplate assay’s one should be aware that lipophilic and volatile chemicals might be underestimated in their toxicity, which could be indicated from evaluating related physicochemical properties modelled from structural information prior to an experimental investigation.     

矿区铁路安全管理信息系统的研究

针对我国矿区铁路运输安全管理落后的现状,分析矿区铁路运输安全管理的主要特点,设计了矿区铁路安全管理信息系统的总体结构。以徐沛铁路运输系统为例,基于B/S模式开发了徐沛铁路安全管理信息系统,实现了对职工的安全培训考核、职工安全档案管理、运输事故信息的录入与统计分析、各类安全文档的管理、技术措施的审批和安全直通车的功能。该系统已在徐沛铁路运输安全管理中得到成功应用,也可应用于其他矿区铁路安全管理。     

Laboratory longitudinal diffusion tests: 2. Parameter estimation by inverse analysis

This study focuses on the verification of test interpretations for different state analyses of diffusion experiments. Part 1 of this study identified that steady, quasi-steady and equilibrium state analyses for the through- and in-diffusion tests with solution reservoirs are generally feasible where the tracer is not highly sorptive. In Part 2 we investigate parameter identifiability in transient-state analysis of reservoir concentration variation using a numerical approach. For increased generality, the analytical models, objective functions and Jacobian matrix necessary for inverse analysis of transient-state data are reformulated using unified dimensionless parameters. In these dimensionless forms, the number of unknown parameters is reduced and a single dimensionless parameter represents the sorption property. The dimensionless objective functions are evaluated for individual test methods and parameter identifiability is discussed in relation to the sorption property. The effects of multiple minima and measurement error on parameter identifiability are also investigated. The main findings are that inverse problems for inlet and outlet reservoir concentration analyses are generally unstable and well-posed, respectively. Where the tracer is sorptive, the inverse problem for the inlet reservoir concentration analysis may have multiple minima. When insufficient measurement data is collected, multiple solutions may result and this should be taken into consideration when inversely analyzing data including that of inlet reservoir concentration. Verification of test interpretation by cross-checking different state analyses is feasible where the tracer is not highly sorptive. In an actual experiment, test interpretation validity is demonstrated through consistency between theory and practice for different state analyses.     

中华鳖出血性肠炎的药物防治研究

对由实验定筛选的中华鳖出血肠炎病病原菌的最佳防治药物为本正交试验的第6组,即A2B3C1,其抑菌圈达21.5mm.临床应用中采用中西结合复方制剂,即用第6组试验药物再加上中药白头翁汤(加减),其疗效更佳.     

地震学综合定量预报方法在华东地区地震预测中的应用研究

本文应用地震学综合定量预报方法,结合区域地震活动特点,在华东地区进行了分区实际预报效能检验.结果表明该方法在华东地区有5个区域通过R值检验,效果较好,有4个区域未能通过R值检验;并对通过检验的区域地震活动趋势进行了分析.     

建筑物基底反力的实时监测与分析

以某大楼的基底反力监测为例 ,阐述了建筑物基底反力实时监测的原理与方法。监测分析结果表明 ,对在建建筑物进行的基底反力实时监测直接反映了土体受力状态 ,可间接得出桩基的受力情况。     

外力作用下管道内表面缺陷处裂纹扩展研究

为提高油气管道安全风险评估的准确性,以含内表面缺陷管道为研究对象,利用ABAQUS软件线弹性及弹塑性分析不同尺寸缺陷对裂纹萌生特征的影响;采用扩展有限元法,建立管道3点弯曲模型与全尺寸静载物理试验,并非线性拟合裂纹扩展尺寸,研究内表面缺陷处裂纹扩展情况.研究结果表明:内表面缺陷中心处极易萌生裂纹,随着缺陷尺寸的增大,裂...     

水样发光细菌急性毒性测试的方式与表征

选取不同类型的环境和污染源水样开展了发光细菌毒性测试,针对不同环境管理目标,探讨了急性毒性定量表征方式。发光细菌急性毒性测试在环境应急监测、水源早期生物预警中,采用绘制毒性预警基线图的方式比较可行。在污染源监督监测中,对于发光抑制率低于60%、无法求出EC50的样品,采用等效毒性参照物的质量浓度表征废水的毒性;对于发光抑制率高于60%,采用稀释因子表示样品的毒性更直观可靠。     

Validation of a booted finite element model of the WIAMan ATD lower limb in component and whole-body vertical loading impacts with an assessment of the boot influence model on response

Objective: A novel anthropomorphic test device (ATD) representative of the 50th percentile male soldier is being developed to predict injuries to a vehicle occupant during an underbody blast (UBB). The main objective of this study was to develop and validate a finite element (FE) model of the ATD lower limb outfitted with a military combat boot and to insert the validated lower limb into a model of the full ATD and simulate vertical loading experiments.

Methods: A Belleville desert combat boot model was assigned contacts and material properties based on previous experiments. The boot model was fit to a previously developed model of the barefoot ATD. Validation was performed through 6 matched pair component tests conducted on the Vertically Accelerated Loads Transfer System (VALTS). The load transfer capabilities of the FE model were assessed along with the force-mitigating properties of the boot. The booted lower limb subassembly was then incorporated into a whole-body model of the ATD. Two whole-body VALTS experiments were simulated to evaluate lower limb performance in the whole body.

Results: The lower limb model accurately predicted axial loads measured at heel, tibia, and knee load cells during matched pair component tests. Forces in booted simulations were compared to unbooted simulations and an amount of mitigation similar to that of experiments was observed. In a whole-body loading environment, the model kinematics match those recorded in experiments. The shape and magnitude of experimental force–time curves were accurately predicted by the model. Correlation between the experiments and simulations was backed up by high objective rating scores for all experiments.

Conclusion: The booted lower limb model is accurate in its ability to articulate and transfer loads similar to the physical dummy in simulated underbody loading experiments. The performance of the model leads to the recommendation to use it appropriately as an alternative to costly ATD experiments.     

Zr掺杂TiO_2介孔材料光催化降解头孢氨苄

采用固相合成法制备了Zr掺杂TiO_2(Zr-TiO_2),运用XRD技术对其进行了表征,并将其用于水中头孢氨苄的光催化降解,通过单因素实验及正交实验优化了光催化反应条件。结果表明:制备的Zr-TiO_2为锐钛矿型介孔材料,孔径约为8.12 nm;各因素对头孢氨苄去除率的影响由大到小依次为光照时间、Zr-TiO_2投加量、初始头孢氨苄质量浓度;在Zr-TiO_2投加量为1.5 g/L、初始头孢氨苄质量浓度为10 mg/L、溶液pH为7.0、光照(300 W UV)时间为1.5 h的优化条件下,头孢氨苄去除率高达99.46%;Zr-TiO_2光催化剂的重复使用性能良好。