恶臭假单胞菌Pseudomonas putida 5-x细胞壁膜系统的Cu2+吸附性能

对1株从电镀废水中分离出的革兰氏阴性菌恶臭假单胞菌P.putida 5-x的细胞表面组分对Cu²⁺的吸附性能进行了分析研究.结果表明,分离的P.putida 5-x细胞壁膜的Cu²⁺吸附容量是完整细胞的5倍之多.细胞表面组分如肽聚糖层、细胞外膜和细胞内膜在P.putida 5-x细胞壁膜的Cu²⁺吸附过程中都发挥了作用.肽聚糖层、细胞外膜和内膜在P.putida 5-x细胞壁膜中的含量依次为细胞内膜>外膜>肽聚糖层,而它们的Cu²⁺吸附容量的大小依次为肽聚糖层>外膜>内膜.在P.putida 5-x细胞壁膜对Cu²⁺的吸附过程中,肽聚糖层贡献了不到15%的吸附容量,而细胞外膜和内膜分别贡献了30%～35%和25%～30%.P.putida 5-x细胞外膜中的磷脂含量明显比其它报道的革兰氏阴性细菌的细胞外膜高,这可能是P.putida 5-x细胞外膜具有较高Cu²⁺吸附容量的主要原因,并由此导致P.putida 5-x细胞壁膜的高Cu2+吸附容量.     

Titration Curves: A Useful Instrument for Assessing the Buffer Systems of Acidic Mining Waters (10 pp)

Background, Aims and Scope The acidification of mine waters is generally caused by metal sulfide oxidation, related to mining activities. These waters are characterized by low pH and high acidity due to strong buffering systems. The standard acidity parameter, the Base Neutralization Capacity (BNC) is determined by endpoint titration, and reflects a cumulative parameter of both hydrogen ions and all buffering systems, but does not give information on the individual buffer systems. We demonstrate that a detailed interpretation of titration curves can provide information about the strength of the buffering systems. The buffering systems are of importance for environmental studies and treatment of acidic mining waters. Methods Titrations were carried out by means of an automatic titrator using acidic mining waters from Germany and Canada. The curves were interpreted, compared with each other, to endpoint titration results and to elemental concentrations contained therein. Results and Discussion The titration curves were highly reproducible, and contained information about the strength of the buffer systems present. Interpretations are given, and the classification and comparison of acidic mining waters, by the nature and strength of their buffering systems derived from titration curves are discussed. The BNC-values calculated from the curves were more precise than the ones determined by the standard endpoint titration method. Due to the complex buffer mechanisms in acidic mining waters, the calculation of major metal concentrations from the shape of the titration curve resulted in estimates, which should not be confused with precise elemental analysis results. Conclusion Titration curves provide an inexpensive, valuable and versatile tool, by which to obtain sophisticated information of the acidity in acidic water. The information about the strength of the present buffer systems can help to understand and document the complex nature of acidic mining water buffer systems. Finally, the interpretation of titration curves could help to improve treatment measurements and the ecological understanding of these acidic waters.     

The environmental impact of industrialization in East Asia and strategies toward sustainable development

The East Asian economy has been growing fast in recent years, and environmental stresses are building up rapidly. Transboundary air pollution, water shortages, drinking water contamination, freshwater and marine pollution, deforestation, climatic disasters, and other environmental problems are becoming serious threats to the well-being of people in this densely populated region. The ESI (environmental sustainability index) reported by the World Economic Forum in 2005 is a good indicator of the environmental status of the region; most East Asian countries ranked at the bottom. East Asia is not moving toward a sustainable society, and the environment will not sustain the current rate of economic development for long. The traditional culture of East Asia used to be sustainable, so we can learn much from our traditions. Land use should be planned from an ecological point of view so as to best preserve the land’s productivity and stability. There should be definite goals as to where and how much to preserve the three important ecological bases: forests, coastal wetlands and agricultural farms. The forest is the base for the terrestrial ecosystem, including flood control, water resources, and climate; the coastal wetland is the base for the marine ecosystem; farmland is the base for producing food. Within these defined goals, limits should be set on how much land can be utilized for activities like urban development, manufacturing, and recreation. Limits on the pollution load resulting from such activities should be set so as not to irreversibly damage the environment. Economic development should be planned to allow the use of energy and resources only after satisfying these constraints.

淮北市水资源保护与规划

系统论述了淮北市水资源现状和存在问题,提出了保护水资源环境、促进可持续发展而采取的有效工程措施。     

新疆麻黄资源的开发利用与保护

介绍了新疆麻黄资源的种类、分布及开发利用现状,指出新疆野生麻黄分布总面积约167hm2×104hm2,可利用量仅为蕴藏量的30%。针对目前麻黄资源严重破坏的现状,提出了今后合理开发利用和保护的具体措施。     

三峡库区陆生植物迁地保存初报

三峡植物物种多样性保护,重点在珍稀濒危植物,但对建群种和有实用价值的种也进行收集保存。7年来试验站保存三峡库区植物200余种,迁移成活率93%,有8种未成功。（1）到2002年已有30余种植物正常开花结实（其中木本植物24种）,可以选择繁殖试验,以达到保存的目的;（2）选择了8种植物,进行生长观测,在人为控制下,生长速度满意;（3）进行了部份种类的扦插繁殖和孢子繁殖,获得了相关结果;（4）少数种类收集遇到极大困难,需要继续努力。三峡库区生物多样性研究与保护,存在一些问题与困难,如漏项问题、库区物种问题、外来种问题、隔离对生物多样性的影响问题、生物多样性的功能问题等需要进一步研讨,从而使库区物种得以保存,环境得以优化,生物资源得到持续利用。     

Fe和Fe2+对混合细菌产氢发酵的影响

在研究Fe粉剂量和Fe²⁺浓度对混合细菌产氢发酵的影响基础上,确定Fe和Fe²⁺促进混合细菌产氢能力的最佳阈值,并对乙醇型发酵菌群在不同Fe粉和Fe²⁺浓度下的产氢量和最大比产氢速率进行考察和对比.结果表明,Fe粉和Fe²⁺对乙醇型发酵菌群的产氢能力均有明显的促进作用.以葡萄糖为底物,投加Fe²⁺试验中,Fe²⁺浓度200mg/L获得最大产氢量143.7mL/g,较对照组提高32%;Fe²⁺浓度50mg/L获得单位VSS最大比产氢速率21.2 mL/(h·g),较对照组提高33%.投加单质Fe试验中,Fe粉剂量1000mg/L获得最大产氢量156.1mL/g,较对照组提高44%;Fe粉剂量500mg/L获得单位VSS最大比产氢速率23.5mL/(h·g),较对照组分别提高47%.单质Fe浓度高于50mg/L时,对发酵菌群产氢的促进作用要优于同浓度下的Fe²⁺.同时对混合细菌中铁的全量和形态分布进行了考察.     

云南省锡矿资源与可持续发展

云南省锡矿资源储量居全国前列,锡产量在世界锡工业中举足轻重。虽然云南省锡矿资源开发程度高,但“四矿”(矿业、矿山、矿工、矿城)问题严重,锡矿资源的开发与可持续发展矛盾突出。需加强锡矿资源的地质勘探和宏观调控,加大人才、资金、科技投入和产业支持力度,将锡矿资源优势转化为锡工业优势,应充分利用云南省的地理优势扩大对外交流,实现锡矿资源的可持续发展。     

浅析建立排污权有偿交易制度

随着工业化、城市化的快速推进,环境容量资源有限和社会经济持续较快发展的矛盾将长期存在。在充分运用必要的行政和法律手段的同时,应注重运用经济手段———排污权有偿使用和交易进行环境保护,使外部环境内部化,树立"容量有限、资源有价、使用有偿"的环境资源价值理念,推动企业自主创新和技术进步,降低企业内部治理成本,调动污染减排积极性,促进环境管理方式从粗放型向精细化转变,全面提升环境管理水平。     

基于灰色聚类分析的轨道交通应急能力综合评价

通过分析轨道交通应急能力的影响因素,建立多层次的评价指标体系,引入灰色聚类分析对轨道交通应急能力进行综合评价。针对传统灰色聚类分析计算复杂、繁琐的缺点,在对二级指标的评价中对聚类指标规范化,采用区间化白化权函数进行简化处理,然后对得到的一级指标综合值再次进行聚类分析。结果表明,聚类分析在多层次综合评价中具有较好的实用性和可操作性。