悬浮载体生物膜内硝化菌群空间分布规律

利用16S rRNA寡核苷酸探针荧光原位杂交和共聚焦激光扫描显微镜联用技术,对悬浮载体生物膜内硝化菌群的空间分布规律进行了分析.试验采用3组结构完全相同的悬浮载体生物膜反应器,每个反应器的曝气区为6L,沉淀区为2L,水力停留时间为1.0h,3个反应器的进水COD/NH₄⁺-N分别为15、10和5,从反应器中取出载体颗粒表面的生物膜进行分析,研究各反应器中生物膜的微生物群落结构的变化规律.结果表明,SCBR内载体表面生物膜的总体厚度在80～120μm左右,氨氧化菌和亚硝酸盐氧化菌主要分布在生物膜表面的20～30μm左右范围内.随着进水中COD/NH₄⁺-N的增加,氨氧化菌和亚硝酸盐氧化菌在整个生物膜中所占的比例逐步下降.     

Aspects of biopsy procedures prior to preimplantation genetic diagnosis

Today, preimplantation genetic diagnosis (PGD) is offered in over 40 centres worldwide for an expanded range of genetic defects causing disease. This very early form of prenatal diagnosis involves the detection of affected embryos by fluorescent in situ hybridization (FISH) (sex determination or chromosomal defects) or by polymerase chain reaction (PCR) (monogenic diseases) prior to implantation. Genetic analysis of the embryos involves the removal of some cellular mass from the embryos (one or two blastomeres at cleavage-stage or some extra-embryonic trophectoderm cells at the blastocyst stage) by means of an embryo biopsy procedure. Genetic analysis can also be performed preconceptionally by removal of the first polar body. However, additional information is then often gained by removal of the second polar body and/or a blastomere from the embryo. Removal of polar bodies or cellular material from embryos requires an opening in the zona pellucida, which can be created in a mechanical way (partial zona dissection) or chemical way (acidic Tyrode's solution). However, the more recent introduction of laser technology has facilitated this step enormously. Different biopsy procedures at different preimplantation stages are reviewed here, including their pros and cons and their clinical applications. The following aspects will also be discussed: safety of zona drilling by laser, use of Ca²⁺/Mg²⁺-free medium for decompaction, and removal of one or two cells from cleavage-stage embryos. Copyright © 2001 John Wiley & Sons, Ltd.     

激光质谱法对机动车尾气中污染物的测量

本文详细介绍了一种全新的污染物测试方法———激光质谱法的原理和特点。我们应用这一方法对机动车尾气进行了初步探测 ,得到了 2 6 6nm激光作用下汽车尾气的激光电离质谱图 ,并对摩托车发动机运行状态与苯系列污染物排放量之间的关系进行了初步的在线测量     

速差自控器动态性能试验方法的改进

为施工人员选择和配置安全可靠的个人防坠器,每年送到具有相应资质的检测单位进行强制检定,是防止高处坠落事故的重要手段。某型速差自控器在冲击试验中发生了钢丝绳断裂事故,为了查找和分析事故的真正原因,对不同厂家的速差自控器抽样,在相同试验方法和检测条件下进行冲击试验,并根据相关的标准对试验结果进行力学性能对比分析研究,找到了造成钢丝绳断裂事故的主要原因是该类型速差自控器使用的钢丝绳直径偏细以及缺少内部或外部缓冲器,研究中还发现部分国内标准技术要求不够明确,制造、检测和使用方对速差自控器相关标准理解存在偏差,是造成本次事故的间接原因。针对现有的部分速差自控器产品存在的质量问题,并根据现行最新标准和实际工作条件,提出改进了的检测方法和防范措施,从源头上防范坠落事故。     

Continuous live cell imaging of cellulose attachment by microbes under anaerobic and thermophilic conditions using confocal microscopy

Live cell imaging methods provide important insights into the dynamics of cellular processes that cannot be derived easily from population-averaged datasets.In the bioenergy field,much research is focused on fermentation of cellulosic biomass by thermophilic microbes to produce biofuels;however,little effort is dedicated to the development of imaging tools to monitor this dynamic biological process.This is,in part,due to the experimental challenges of imaging cells under both anaerobic and thermophilic conditions.Here an imaging system is described that integrates confocal microscopy,a flow cell device,and a lipophilic dye to visualize cells.Solutions to technical obstacles regarding suitable fluorescent markers,photodamage during imaging,and maintenance of environmental conditions during imaging are presented.This system was utilized to observe cellulose colonization by Clostridium thermocellum under anaerobic conditions at 60℃.This method enables live cell imaging of bacterial growth under anaerobic and thermophilic conditions and should be widely applicable to visualizing different cell types or processes in real time.     

传输法对不同方法制备频率选择表面的测试应用

随着频率选择表面(FSS)的广泛应用,精确的制备和测量技术验证理论分析日趋重要。本文采用丝网印刷法和激光加工法实现了计算中采用的频率选择表面周期单元的制备。基于Agilent E8257D信号发生器、Agilent E7405A频谱分析仪,采用传输法对制备的不同尺寸频率选择表面进行测试,得到了各种频率选择表面的频响特性。通过比较测试和仿真结果,验证了频率选择表面结构参数对频响特性的影响规律的正确性。同时,比较测试和仿真结果,得出激光加工法获得的频率选择表面精度更高的结论。     

Novel method for in situ visualization of polycyclic aromatic hydrocarbons in mangrove plants

Two-photon laser confocal scanning microscopy (TPLCSM) was first used to visualize the uptake and movement of naphthalene (NAP), phenanthrene (PHE), and pyrene (PYR), from separately contaminated hydroponics solutions into living Kandelia candel (L.) Druce seedlings. With this non-destructive and non-chemical extraction technique, the experimental results revealed that three types of polycyclic aromatic hydrocarbons (PAH) were observed moving into the K. candel and its translocation to radicle, hypocotyl and leaf, and the transmission rates of these PAH in the K. candel were in the order of NAP?>?PHE?>?PYR. Data demonstrate the manner in which PAH enter, transport, and distribute within the K. candel, and provided us some valuable information on uptake and translocation mechanism of PAH. These findings may help to optimize the phytoremediation strategies of PAH in mangrove wetlands.     

Kinetics and mechanisms of reactions for hydrated electron with chlorinated benzenes in aqueous solution

The reactions between chlorinated benzenes (CBzs) and hydrated electron (eaq-) were investigated by the electron beam (EB) and laser flash photolysis (LFP) experiments. Under the EB irradiation, the effects of irradiation dose, initial concentration and the number of Cl atoms on the removal efficiencies were further examined. At 10 kGy, the removal efficiencies of mono-CB, 1,3-diCB, 1,2-diCB and 1,4-diCB were 41.2%, 87.2%, 84.0%, and 84.1%, respectively. While irradiation dose was 50 kGy, the removal efficiencies increased to 47.4%, 95.8%, 95.0%, and 95.2%, respectively. Irradiation of CBzs solutions has shown that the higher the initial concentration, the lower the percentage of CBzs removal. In addition to this, the dechlorination efficiencies of 1,2-dichlorobenzene (1,2-diCB), 1,3-dichlorobenzene (1,3-diCB) and 1,4-dichlorobenzene (1,4-diCB) were much higher than that of chlorobenzene (mono-CB). The kinetics of the reactions was achieved with nanosecond LFP. The rate constants of second-order reaction between eaq- with mono-CB, 1,2-diCB, 1,3-diCB and 1,4-diCB were (5.3±0.4) × 10⁸, (4.76±0.1) × 10⁹, (1.01±0.1) × 10¹⁰ and (3.29±0.2) × 10⁹ L·mol^-1·s^-1, respectively. Density functional theory (DFT) calculations were performed to determine the optical properties of unstable CBzs anion radicals, and the main absorption peaks lied in the range of 300–550 nm. The primary reaction pathway of CBzs with eaq- was gradual dechlorination, and the major products were Cl^- and benzene (CBzs(-Cl^-)). Furthermore, biphenyl (or chlorobiphenyl) was observed during the LFP, which was probably formed by recombination of benzene radicals.     

激光点火器耐压密封及其影响

目的促进激光点火技术在安全弹药领域的应用。方法从激光点火器作用后承受高动态压力的需求出发,研究基于玻璃封接技术的激光点火器密封性能及其影响因素。确定采用箱式电阻炉对激光点火器壳体与透镜间进行玻璃密封的温度为430℃,保温时间为13～15min。对不同始发装药、不同密封工艺的激光点火器透镜发火后,其表面光学膜层变化、与激光点火器相连接的光纤端面损伤程度进行分析,研究点火前后承受气压性能变化。结果始发装药为BPN等点火类药剂时,激光点火器具有较高的动态密封性能,可稳定达到30 MPa下2 min不泄露;始发装药为BNCP等起爆类药剂时,随着装药量的增大,密封性能变差。结论改善玻璃封接工艺有利于提高密封性,如封接长度变长,发火后耐压密封性会有所提升。