大凉疣螈脑源性神经营养因子(BDNF)基因的克隆与序列分析

大凉疣螈 (Ｔｙｌｏｔｏｔｒｉｔｏｎｔａｌｉａｎｇｅｎｓｉｓ)属于两栖纲有尾目蝾螈科 ,仅分布在我国四川省西南部 ,活动范围狭窄 ,数量有限 ,是我国二级保护动物[1] .两栖动物在生物进化中是承上启下的关键动物 ,它们开启了脊椎动物登上陆地的历史 ,而且是真正陆生脊椎动物—爬行动物的祖先 ,其生理、结构等方面与鱼类和爬行类都有着明显的差别 .脑源性神经营养因子 (ｂｒａｉｎ ｄｅｒｉｖｅｄｎｅｕｒｏｔｒｏｐｈｉｃｆａｃｔｏｒ,ＢＤＮＦ)是神经营养因子家族的一员 ,ＢＤＮＦ在脊椎动物胚胎神经系统的发育、分化及在成体神经系统维…     

无指盘臭蛙皮肤抗茵肽的分离纯化与氨基酸序列测定

在抗菌活性跟踪下对四川冕宁产无指盘臭蛙（Odorranagrahami）皮肤分泌物进行一系列层析分离，最终纯化得到4种抗菌肽，分别命名为Odorgrin A、Odorgrin B、OdorgrinC和OdorgrinD．质谱测得单一同位素分子量恤分别为3364．8、3817．9、4859．4和3287．9．Edman降解法测得OdorgrinA的氨基酸序列为GLLDTFKNLALNAAKSAGVSV—LNSLSCKLSKTC．将以上结果与国内外对该物种抗菌肽研究的已有结果进行了比较分析，证明本研究分离到的4种抗菌肽与前人分离到的部分抗菌肽间具有一致性．     

CMS高粱保持系叶绿体基因ps1A1和ps1A2片段的克隆与序列比对

以15种包括同质异核和同核异质高粱材料的总：DNA为模板进行RAPD分析，196个随机引物中，引物Y-19扩增得到一个很有规律的差异片段SAY-192300。该片段只出现在4个保持系(B)和3个恢复系(R)中，而不出现在6个不育系(A)和2个杂种F1中，进一步对cms—Al、cms—A2两套8个材料(A／B／R／F1)的总DNA、mtDNA和cpDNA用引物Y-19进行扩增，发现片段SAY-192300仅在cpDNA得到，Southern杂交结果证实了片段SAY-192300的叶绿体来源，同时也表明在cms胞质中该片段所在区未发生插入、缺失等大的结构变异,DNA序列测定结果表明，该片段为叶绿体ps1A1和ps1A2基因的部分序列。图6表2参19。     

亚间冰期以来长江入海口下切河谷充填型沉积体系与生物气聚集

分析了江苏省启东市北新镇NTZK01孔岩心沉积特征,结合该孔45个有机碳样品和14个样品的渗透率值,讨论亚间冰期(LG)和冰后期(PG)海侵旋回的层序地层特征及浅层生物气的勘探前景。研究表明,研究区在亚间冰期(LG)海侵旋回,海平面快速下降,地层遭受侵蚀,仅保留部分河床沉积,其最大海泛面也未保存。研究区在冰后期(PG)海侵旋回,发育了一套完整的海侵-海退旋回,下切河谷区自下而上依次发育河流相、浅海相和三角洲相。底部侵蚀面为冰后期(PG)海侵旋回的底界面,最大海泛面发育在淤泥质粘土层中,最大海泛面以下为海侵旋回,最大海泛面以上为海退旋回。     

高压输电线路并列架设空间工频电场研究

利用等效电荷法计算多线路并列架设空间工频电场。结果表明,并列架设输电线路之间的工频电场会出现不同程度的叠加或削减,进而影响线路下方工频电场的强度和变化趋势。提出了多线路并列架设的线路空间布置建议,为输电线路环境影响评价模拟类比线路选取提供参考。     

Metabolic fate of the 14C-labeled herbicide clodinafop-propargyl in a sediment–water system

The metabolic fate of ¹⁴C-phenyl-labeled herbicide clodinafop-propargyl (¹⁴C-CfP) was studied for 28 days in lab assays using a sediment–water system derived from a German location. Mineralization was 5.21% of applied ¹⁴C after 28 days exhibiting a distinct lag phase until day 14 of incubation. Portions of radioactivity remaining in water phases decreased at moderate rate to 18.48% after 28 days; 62.46% were still detected in water after 14 days. Soxhlet extraction of the sediment using acetonitrile released 35.56% of applied ¹⁴C with day 28, while 33.99% remained as non-extractable residues. A remarkable increase of bound ¹⁴C was observed between 14 and 28 days correlating with the distinct increase of mineralization. No correlation was found throughout incubation with microbial activity of the sediment as determined by dimethyl sulfoxide reduction. Dissolved oxygen and pH value of water phases remained almost constant for 28 days. Analyses of Soxhlet extracts of the sediment and ethyl acetate extracts of water phases by radio-TLC and radio-HPLC revealed that CfP was rapidly cleaved to free acid clodinafop (Cf), which was further (bio-) transformed. DT₅₀ values (based on radio-HPLC) were below 1 day (CfP) and slightly above 28 days (Cf). Further metabolites were not detected. Fractionation of humic and non-humic components of the sediment demonstrated that CfP's non-extractable residues were predominantly associated with fulvic acids up to 14 days of incubation (3.36%), whereas after 28 days, the majority of radioactivity was found in the humin/mineral fraction (13.30% of applied ¹⁴C). Due to high-performance size-exclusion chromatography of the fulvic acids fraction derived from assays incubated for 28 days, this portion of ¹⁴C was firmly, possibly covalently bound to fulvic acids and did not consist of CfP or Cf. Using an isolation strategy comprising preincubation of sediment with CfP and mineralization of ¹⁴C-CfP as criterion, a microorganism was isolated from the sediment examined. It grew on ¹⁴C-CfP as sole carbon source with evolution of ¹⁴CO₂. The bacterium was characterized by growth on commonly used carbon sources and 16S rDNA sequence analysis. Its sequence exhibited high similarity with that of Nocardioides aromaticivorans strain H-1 (98.85%; DSM 15131, JCM 11674).     

六种重金属对大肠杆菌体内质粒DNA一级结构的影响

将质粒ｐＢＲ３２２、ｃ－Ｍｙｃ、ｐＣ５３－ＳＮ_３、ｐＴＢ２９转入大肠杆菌（Ｅｓｃｈｅｒｉｃｈｉａｃｏｌｉ）ＨＢ１０１中使其各自分别在含亚致死浓度Ｐｂ²⁺、Ｃｄ^2-、Ｚｎ²⁺、Ｃｕ²⁺、Ｍｎ²⁺、Ｈｇ²⁺的液体氨苄ＬＢ培养基中,振荡培养２４ｈ后,再将这些质粒抽提出来,通过限制性内切酶分析,研究了这些重金属离子对大肠杆菌体内质粒ＤＮＡ分子一级结构的影响。结果表明,在供试的６种重金属离子中只有Ｈｇ^２＋处理可明显导致质粒ＤＮＡ序列的改变。此外,供试６种重金属离子连续处理２４ｈ未发现可明显导致大肠杆菌体内质粒ＤＮＡ链的随机断裂。     

大连港专用矿石码头污水处理工程设计

采用沉砂、序批调节沉淀及浅层介质过滤的物化组合方式处理大连港专用矿石码头洗矿污水,介绍了各处理构筑物、运行参数.运行结果表明,用该法处理洗矿污水,其出水水质可达<生活杂用水水质标准>(CJ25.1-89)中绿化用水标准(其中悬浮物不大于10 mg/L).     

Isolates identification and characteristics of microorganisms in biotrickling filter and biofilter system treating H2S and NH3

A combination system of biotrickling filter （BTF） and biofilter （BF）, adopting surfactant-modified clinoptilolite and surfactant- modified wood chip as the media respectively, was applied to treat H2S and NH3 simultaneously. The identification and sole carbon sources utilization patterns of isolates in the combination system were studied by Biolog system. The isolates were identified as Bacillus sphaericus, Geobacillus themoglucosidasius （55℃） and Micrococcus luteus （ATCC 9341） in BTF, and Aspergillus sydowii （Bainier ＆ Sartory） Thom＆ Church in BE Among 95 substrate classes supplied by Biolog system, the carboxylic acids and methyl esters had the highest utilization extent for the four species, followed by the amino acids and peptides. The descending sequence of carbon sources utilization capability of isolates was A. sydowii （52.6%）, M. luteus （39.5%）, B. sphaericus （21.6%）, and G. thermoglucosidasius （17.7%）.     

东北北部地区地震活动与震后早期趋势判断

研究了 190 0年以来我国东北北部地区浅源中强震活动的特征。结果表明 ,地震活动具有突发性、成组性和序列地震数量少的特点。应根据本区地震活动特点 ,开展震后早期趋势预测 ,以最大限度地减轻地震灾害。