The suitability of the primmorphs system as a good model for biotechnological applications led researchers on primmorphs to look for a medium to stimulate cell proliferation and therefore growth of aggregates. Recent efforts have focused on the use of Fe+3 and Si that, supplemented to cell culture medium, were found to be promising for growth and morphogenesis of the sponge Suberites domuncula. In this work, we analysed the effect of iron and dissolved silica on primmorphs of Petrosia ficiformis, by testing them at different concentrations in successive experiments. The purpose of these experiments was to test their effect on primmorphs and individuate their optimal concentration for this species. Our results suggest a negative effect of iron on primmorphs of P. ficiformis and a positive effect of silica on primmorphs size and spiculogenesis at a concentration of 120 μM. 相似文献
Formaldehyde has been classified in group 1 as a potential source of human carcinogen by the International Agency for Research on Cancer. In this study, we evaluated the DNA damage in workers who were occupationally exposed to formaldehyde during the manufacture of melamine dinnerware.
Age, sex, smoking habits, and socioeconomic status were matched by the controls (n = 34) of workers not occupationally exposed to formaldehyde. The median value of formaldehyde time weighted average exposure in three workshops was 0.086 mg/m3. No significant difference was observed between sex and smoking habit in the assessed workers. Alkaline comet assay of peripheral blood lymphocytes was used to determine the DNA damage in the subjects. Olive moment was significantly higher in the exposed group compared with the control group.
Overall, the results suggest that respiratory exposure to low concentrations of formaldehyde causes DNA damage in peripheral blood lymphocyte. Therefore, exposure to formaldehyde should be reduced to the lowest possible level. 相似文献