煤矸石微生物脱硫试验研究

采用本试验室自主分离筛选出的氧化硫硫杆菌,通过煤矸石浸泡的微生物脱硫和煤矸石柱状淋洗的微生物脱硫试验,研究该菌株对大武口高硫煤矸石的脱硫效果.煤矸石浸泡试验表明,微生物对煤矸石脱硫效果明显,浸泡煤矸石2 h后脱硫量达0.79 g/L,脱硫率达24.5%,21 d持续稳定升高,脱硫率达28.3%,为煤矸石的快速脱硫提供了技术支持.煤矸石柱状淋洗试验表明,前9 d是氧化硫硫杆菌脱硫的活跃期,该菌株有较高的氧化煤矸石的能力.     

Parental mosaic trisomy 21 detected following maternal cell contamination of an amniotic fluid specimen from a normal male pregnancy

We report a case of maternal mosaic trisomy 21 ascertained at prenatal diagnosis as a result of maternal cell contamination of an amniotic fluid sample. A 34 year old female was referred for karyotyping because of a previous trisomy 21 pregnancy. Chromosome analysis of primary in situ cultures showed a karyotype of 47,XX, + 21[6]/46,XY[32]/46,XX[2]. Molecular testing demonstrated maternal cell contamination of the amniotic fluid sample and G-banded karyotyping of maternal blood showed that 3/200 cells had trisomy 21, consistent with the mother being a Down syndrome mosaic. A normal male baby with a 46,XY chromosome complement was delivered at 30 weeks. This case emphasises the need for close collaboration between cytogenetic and molecular genetics laboratories in resolving unusual cases of mosaicism. Copyright © 2007 John Wiley & Sons, Ltd.     

Combustion performance and emission characteristics on HCCI engine of waste plastic pyrolysis oil biodiesel blends with external PFI and vaporiser

ABSTRACT

Analysis of plastic oil obtained from waste plastic through pyrolysis process, as an alternative to biodiesel is presented in this paper. The HCCI engine is considered for experimental validation of combustion performance and emission characteristics. To accumulate pyrolysis oil as fuel, the design modifications were made in external mixture formation on the existing computerised 4-stroke, single cylinder, water cooled, direct injection kirloskar diesel engine connected with eddy current dynamometer to satisfy HCCI conditions. HCCI engine can be worked on wide assortment of fuels beginning from diesel to different blends (WPPO 5%,10%,15% and 20% by volume) of biodiesel .The designed additional device connected to the engine is utilised for fuel vaporisation and mixture arrangement. In the experimental study, the combustion results were initiate to be of 39.69 % higher Rate of Heat Release (RoHR) for biodiesel HCCI as compared with diesel HCCI. Higher brake thermal efficiency (BTE) was found 37 % without exhaust gas recirculation (EGR) at WPPO 20 % biodiesel blend. And also found 50 % and 65 % reduction in NOx emission and 18 % and 28 % reduction in smoke opacity are obtained for biodiesel vapour induction without EGR and biodiesel vapour induction with 15 % EGR as compared with diesel fuel. The CO (0.34 %), and UHC (2.15 %) emissions are increases with 15 % EGR, but the emissions are within the standard limits specified by the emissions standards.     

Alteration of brain electrical activity by electromagnetic field

To better understand the affect of different levels of EMF on one’s brain activity, the Hodgkin–Huxley model has been used to describe the generation of an active potential in the nerve cell membrane. The theoretical calculations have shown that by the simultaneous affect of both passing an electrical potential and external very low frequency AC, one’s excitation pulses may be suppressed beginning with a certain level of external current. The level of this current depends on the frequency. It is postulated that to eliminate this suppression, the brain increases its normal level relatively to the lower limit. It is also postulated that the brain “tunes up” in the presence of external AC, by increasing the amplitude of its own excitation signals so that suppression does not occur. It is possible that this “tuning” process lasts some time after removing the external EMF as the brain adapts. This has been observed experimentally.     

Catalytic conversion of waste tyres into valuable hydrocarbons

Tyre recycling has become a necessity because of the huge piles of tyres that represent a threat to the environment. The used tyres represent a source of energy and valuable chemical products. Waste tyres were pyrolysed catalytically in a batch reactor under atmospheric pressure. Calcium carbide was used as a catalyst to explore its effect on pyrolysis product distribution. The effect of temperature, amount of catalyst and time on the yields of the pyrolysed products was investigated. Char yield decreased with increase of pyrolysis temperature while total gas and liquid yields increased. The liquid fraction was obtained with boiling point up to 320 °C. The physical and chemical properties of the pyrolysed products obtained were characterized. The catalytic pyrolysis produced 45 wt.% aromatic, 35 wt.% aliphatic and 20 wt.% of polar hydrocarbons. The distillation data showed that ∼80% of oil has boiling point below 270 °C which is the boiling point for 50% of distilled product in commercial diesel oil. The oil fraction was found to have high gross calorific value; GCV (42.8 MJ kg⁻¹). Its Specific gravity, viscosity, Kinematic viscosity, freezing point and diesel index were also within the limits of diesel fuel. The char residues were studied to investigate their characteristics for use as a possible adsorbent. Surface area of char before and after acid demineralization was determined to determine the adsorptive features for waste water treatment.     

梦清园芦苇湿地根际微生物特性研究

本文通过对上海市梦清园芦苇湿地净化苏州河污染水体的示范工程采样分析，结果表明芦苇根际的微生物数量、酶的活性高于非根际的土壤。根际细菌、真菌和放线菌的数量均比非根际多，R／S平均值分别为70．3、9．5、2．9。相对于硝酸细菌而言亚硝酸细菌的根际效应较为明显，无论根际还是非根际的亚硝酸细菌数量均高于硝酸细菌的数量。根际细菌和硝化细菌数量随着水力停留时间增加而增加，而根际反硝化细菌数量则减少。菌剂和酶制剂的加入使得非根际泥样异养细菌、真菌、放线菌和亚硝酸细菌比对照组稍高，硝酸细菌和反硝化细菌则较少；而根际泥样中加菌剂和酶制剂组微生物数量比空白组高出1—2个数量级，在投加菌和酶制剂后酶活性无论在根际还是非根际都得到提高，高于对照组约5—6个酶活单位。     

微生物传感器快速测定水中BOD

采用220B型BOD快速测定仪,对标准样品、地表水、生活污水、工业废水中的BOD进行测定,与五日生化培养法进行了比对分析.结果表明：该方法检出限为0.032mg/L;加标回收率在85%～117%之间;相对误差＜6%,相对标准差＜9%,有较高的精密度、准确度;测定地表水、生活污水与五日生化培养法有较好的可比性,测定工业废水存在一定的局限.同时还阐述了微生物传感器快速测定法测定BOD与五日生化培养法,并对其优缺点做了分析比较.     

Derivated fetal haemoglobin as a marker for red cell age in the human fetus reflecting stimulated or impaired red blood cell production

We have determined whether derivated fetal haemoglobin (dHbF, consisting of glycated and acetylated HbF) can be used as a cell age marker for fetal red blood cells (RBCs). Cord blood was obtained between 19 and 39 weeks of gestation from 28 alloimmunised anaemic fetuses (23 RhD+ and 5 Kell) and from 20 non-anaemic fetuses and newborns (controls). Density gradient centrifugation was applied to 36 samples (20 RhD+, 15 controls and 1 Kell) to obtain fractions of increasing cell age. Blood samples were used for measurements of mean cellular volume (MCV), mean cell haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), pyruvate kinase activity (PK) and derivated fetal haemoglobin (dHbF) by cation-exchange HPLC. Reticulocytes were counted only in the whole blood samples. In all density gradient separated RBC fractions, the values for MCV, MCH and PK activity decreased and those of MCHC and dHbF increased with increasing density (equivalent to increasing cell age). The mean density was lower for RBCs of the anaemic RHD group (1.072±0.007 g/ml) than for the non-anaemic controls (1.077±0.005 g/ml) (p<0.05) The RBC density of the Kell sensitised fetus did not differ from those of the controls. In the control group, the values of the cell age markers in whole blood changed significantly with the gestational age, showing an increase of mean age of the erythrocyte population. The best linear relationship was found for dHbF (y=6.28+0.17*weeks; r=0.84; p<0.001). In the anaemic RhD+ fetuses, the RBC age markers did not change with gestational age; the dHbF percentages were lower, and the MCV, MCH, PK values and the reticulocyte counts were higher than in the controls (0.05<p<0.001). The dHbF values of the Kell sensitised fetuses were above (p<0.01) and the reticulocyte counts were below normal (p<0.05) for gestational age. For the anaemic fetuses, a significant number of the dHbF values (86%) and of the reticulocyte counts (78%) differed from the values of the controls (p<0.01). The dHbF percentages in RhD+ fetuses showed the best correlation with the Hb deficit, which is a measure for anaemia (r=−0.81, p<0.0001). We conclude that the percentage derivated HbF may indicate whether the RBC production is normal for gestational age. It may in that sense reflect stimulated or impaired erythropoiesis in alloimmunised haemolytic anaemia. Copyright © 2001 John Wiley & Sons, Ltd.