Growth, photosynthesis and antioxidant responses of two microalgal species, Chlorella vulgaris and Selenastrum capricornutum, to nonylphenol stress

The effect of nonylphenol (NP) on growth, photochemistry and biochemistry of two green microalgae, Chlorella vulgaris and Selenanstrum capricornutum, and their ability to degrade NP were compared. The 96 h EC₅₀ of C. vulgaris and S. capricornutum were greater than 4.0 and 1.0 mg L⁻¹ NP, respectively, suggesting that the former species was more tolerant to NP. Both microalgae acclimated to NP stress through down-regulating their photosynthetic activities, including antenna size (chlorophyll a content), maximal photochemistry (Fv/Fm) and the light absorbed by PSII (ABS/CS₀), but the dissipation of energy from reaction centres (DI₀/RC) increased with the increase of NP concentrations. In C. vulgaris, the changes of these parameters were more significant than in S. capricornutum and recovered completely after a 96 h exposure. The antioxidant responses, such as GSH content, CAT and POD activities in C. vulgaris increased with the increase of NP concentrations after a 24 h exposure, but these changes disappeared with exposure time and recovered to the control levels after 96 h. In S. capricornutum, although GSH content, CAT and POD activities also increased when exposed to low- to moderate-NP concentrations, these values were significantly reduced at a high concentration (4 mg L⁻¹) even after a 96 h exposure, indicating its antioxidant responses were significantly delayed. It is clear that the more NP-tolerant species, C. vulgaris, acclimated better with a faster recovery of its photosynthetic activity from the NP-induced damage, and exhibited more efficient and rapid responses to NP-induced oxidative stress. C. vulgaris also had a higher NP degradation ability than S. capricornutum.     

Growth and photosynthetic responses to copper in wild grapevine

The present study evaluates the tolerance and accumulation potential of Vitis vinifera ssp. sylvestris under moderate and high external Cu levels. A greenhouse experiment was conducted in order to investigate the effects of a range of external Cu concentrations (0–23 mmol L⁻¹) on growth and photosynthetic performance by measuring gas exchange, chlorophyll fluorescence parameters and photosynthetic pigments. We also measured the total copper, nitrogen, phosphorus, sulphur, calcium, magnesium, iron, potassium and sodium concentrations in the plant tissues. All the experimental plants survived even with external Cu concentrations as high as 23 mmol L⁻¹ (1500 mg Cu L⁻¹), although the excess of metal resulted in a biomass reduction of 35%. The effects of Cu on growth were linked to a reduction in net photosynthesis, which may be related to the effect of the high concentration of the metal on photosynthetic electron transport. V. vinifera ssp. sylvestris survived with leaf Cu concentrations as high as 80 mg kg⁻¹ DW and growth parameters were unaffected by leaf tissue concentrations of 35 mg Cu kg⁻¹ DW. The results of our study indicate that plants of V. vinifera ssp. sylvestris from the studied population are more tolerant to Cu than the commercial varieties of grapevine that have been studied in the literature, and could constitute a basis for the genetic improvement of Cu tolerance in grapevine.     

牛至挥发油对肠炎常见菌的体外抗菌作用

不同浓度牛至挥发油对常见的致肠炎沙门氏菌属、大肠杆菌属和葡萄球菌属等31株菌的抗菌作用研究结果表明，该挥发油对上述31株菌均有不同程度的杀菌和抑菌作用．     

事故树定性分析法在实际运用中存在的问题

事故树分析法是一种应用广泛的系统安全分析方法,特别是其定性分析法在安全评价、事故调查分析中得到普遍运用。但是这种方法在实际运用中存在一些常见的问题,不易引起人们的重视。该文根据目前事故树分析法在实际运用中存在的一些突出问题,从定性分析,即最小割集和最小径集求取、结构重要度排序、事故树结果分析三个方面进行了探究,这些问题的存在说明了只有深刻的理解系统分析方法的内在涵义才能在实践中得到正确运用。文中在提出问题的同时给出了正确的运用步骤,力求使这种方法的运用更加规范、正确。     

基于MRMR-HK-SVM模型的PM2.5浓度预测

以赣州市2017年全年的空气质量和气象数据为研究对象,通过最大相关最小冗余算法（MRMR）提取出最优的特征子集,并将其作为预测模型的输入数据,同时构造混合核函数（HK）对传统的支持向量机模型（SVM）进行改进,最终建立MRMR-HK-SVM模型.实验结果表明,MRMR-HK-SVM模型有着更低的平均绝对误差（MAE）、平均绝对百分比误差（MAPE）和均方根误差（RMSE）,相较于传统SVM模型,预测结果平均绝对误差下降了26.9%,且能更加准确的追踪到PM_2.5浓度的突变时刻.可见,MRMR-HK-SVM模型具有更好的泛化能力,能够更加精确地预测PM_2.5浓度.     

钢筋混凝土板弯曲问题的各向同性化最小平方误差法

利用合适的坐标变换将钢筋混凝土板的弯曲问题变换为各向同性板弯曲问题。由弹性薄板的虚功原理及最小平方误差法计算各向同性板弯曲变形,得到原正交各向异性薄板的挠度方程。算例表明,在近似函数选取合理的情况下,该方法简单,计算时间短,具有较高的精确度。     

浮头式换热器故障树的建立

通过现场调研，运用故障树对浮头式换热器常见故障进行分析，得出浮头式换热器系统的主要故障为腐蚀导致的泄漏。从而，从设计、焊接制造、安装、操作维护等几个环节提出改进措施，为浮头式换热器的可靠性分析提供指导。     

基于最小路集求可靠度函数的系统Bayes置信限评估

阐述了复杂系统的Bayes置信限评估原理,并将其与最小路集求可靠度函数的方法相结合,对已知系统可靠性框图或可靠度函数的复杂系统进行可靠度置信限评估.该方法便于工程应用,对于大型复杂系统可靠性评定,具有重要的参考价值.     

煤炭自然发火事故树分析及预防

为了有效地控制煤炭自然发火,减少矿井火灾的发生,依据系统工程的原理,结合煤炭自燃的条件建立了煤炭自然发火事故树,求解得出了事故树最小径集,利用最小径集得出了各基本事件的结构重要度,从而定性分别对砌碹巷道煤炭自然发火事故、采空区(报废采区)遗煤自然发火事故、采空区(生产采区)遗煤自然发火事故、报废巷道与停工停风独头巷道煤炭自然发火事故进行了分析,得出了引起煤炭自然发火的主要原因,并针对其提出了防治煤炭自然发火的预防措施。     

菝葜乙醇提取物的抑菌作用

用不同浓度的菝葜乙醇提取物对4种细菌、1种酵母菌和1种霉菌进行抑菌实验。结果表明:菝葜乙醇提取物对金黄色葡萄球菌、苏云金芽孢杆菌、大肠杆菌和枯草芽孢杆菌的最低抑菌浓度分别为0 14、0 18、0 46和0 44(g/12ml培养基),菝葜乙醇提取物对产黄青霉和啤酒酵母的生长无明显抑制作用。研究结果对进一步综合开发利用菝葜植物资源具有重要意义。