Abundance and removal of antibiotic resistance genes (ARGs) in the rearing environments of intensive shrimp aquaculture in South China

Although research regarding antibiotic resistance genes (ARGs) in aquaculture environments has gained increasing scientific interest, further studies are required to understand the abundances and removal mechanisms of ARGs during the entire rearing period of shrimp aquaculture. Thus, in this study, abundances, distributions and removal rates of ARGs in different environmental compartments of intensive shrimp farms in South China were investigated during the entire rearing period. The results indicated that sul1 and cmlA were the predominant ARGs in the water and sediment samples. Additionally, the total abundance of ARGs was higher in shrimp pond water than in the source water and farm effluent. Moreover, sediment samples indicated significantly higher ARG abundances than water samples from the shrimp ponds (P?<?0.05). Environmental factors were found to significantly affect the distribution of ARGs in shrimp rearing environments. Furthermore, stable ponds aided the removal of ARGs from shrimp pond water. This study accounted for temporal variations in ARG abundances as well as removal of ARGs in different environmental compartments during the entire shrimp rearing period. However, additional research is required to optimize the water treatment process for removal of ARGs from the aquaculture.     

Bioreduction of hexavalent chromium by Exiguobacterium indicum strain MW1 isolated from marine water of Paradip Port,Odisha, India

Hexavalent chromium-tolerant (1500?mg/L) bacterium MW1 was isolated from harbour water of Paradip Port and evaluated for Cr(VI) reduction potential. The isolate was identified as Exiguobacterium indicum by biochemical and 16S rRNA gene sequence methods. Salt tolerance of the bacterium was evaluated in a wide range of NaCl concentrations (0.5–13%, w/v). The Cr(VI) reduction of the strain was evaluated and optimised with varied Cr(VI) concentrations (100–1000?mg/L), pH (5.0–9.0), temperature (30–40°C) and shaking velocity (100–150?rpm) in two different minimal media (M9 and Acetate). Under optimised conditions, after 192?h of incubation nearly 92%, 50% and 46% reduction in the M9 minimal medium and 91%, 47% and 40% reduction in the acetate minimal medium were observed for 100, 500 and 1000?mg/L of Cr(VI), respectively. The exponential rate equation for Cr(VI) reduction yielded higher rate constant value, that is, 1.27?×?10^?2?h^?1 (M9) and 1.17?×?10^?2?h^?1 (Acetate) in case of 100?mg/L and became lower for 500 and 1000?mg/L Cr(VI) concentrations. Further, the association of bacterial cells with reduced product was ascertained by Fourier transform infrared spectrometer, UV–Vis–DRS and field-emission scanning electron microscope–energy-dispersive X-ray analyses. The above study suggests that the higher reducing ability of the marine bacterium E. indicum MW1 will be suitable for Cr(VI) reduction from saline effluents.     

Subcellular distribution and toxicity of cadmium in Potamogeton crispus L

The submerged macrophyte Potamogeton crispus L. was subjected to varying doses of cadmium (0, 20, 40, 60 and 80 μM) for 7 d, and the plants were analyzed for subcellular distribution of Cd, accumulation of mineral nutrients, photosynthesis, oxidative stress, protein content, and ultrastructural distribution of calcium (Ca). Leaf fractionation by differential centrifugation indicated that 48-69% of Cd was accumulated in the cell wall. At all doses of Cd, the levels of Ca and B rose and the level of Mn fell; the levels of Fe, Mg, Zn, Cu, Mo, and P rose initially only to decline later. Exposure to Cd caused oxidative stress as evident by increased content of malondialdehyde and decreased contents of chlorophyll and protein. Photosynthetic efficiency, as indicated by the quenching of chlorophyll a fluorescence (Fv/Fm, Fo and Fm), decreased significantly, the extent of decrease being directly proportional to the concentration of Cd. Increased amounts of precipitates of calcium were noticed in the treated plants, located either outside the cell membrane or in chloroplasts, mitochondria, the nucleus, and the cytoplasm whereas control plants showed small deposits of the precipitates around surface of the vacuole membrane and in the intercellular space but rarely in the cytoplasm. Photosynthetic efficiency and oxidative stress could be used as indicators of physiological end-points in determining the extent of Cd phytotoxicity.     

Toxicological characterization of the landfill leachate prior/after chemical and electrochemical treatment: A study on human and plant cells

In this research, toxicological safety of two newly developed methods for the treatment of landfill leachate from the Piškornica (Croatia) sanitary landfill was investigated. Chemical treatment procedure combined chemical precipitation with CaO followed by coagulation with ferric chloride and final adsorption by clinoptilolite. Electrochemical treatment approach included pretreatment with ozone followed by electrooxidation/electrocoagulation and final polishing by microwave irradiation. Cell viability of untreated/treated landfill leachate was examined using fluorescence microscopy. Cytotoxic effect of the original leachate was obtained for both exposure periods (4 and 24 h) while treated samples showed no cytotoxic effect even after prolonged exposure time. The potential DNA damage of the untreated/treated landfill leachate was evaluated by the comet assay and cytokinesis-block micronucleus (CBMN) assay using either human or plant cells. The original leachate exhibited significantly higher comet assay parameters compared to negative control after 24 h exposure. On the contrary, there was no significant difference between negative control and chemically/electrochemically treated leachate for any of the parameters tested. There was also no significant increase in either CBMN assay parameter compared to the negative control following the exposure of the lymphocytes to the chemically or electrochemically treated landfill leachate for both exposure periods while the original sample showed significantly higher number of micronuclei, nucleoplasmic bridges and nuclear buds for both exposure times. Results suggest that both methods are suitable for the treatment of such complex waste effluent due to high removal efficiency of all measured parameters and toxicological safety of the treated effluent.     

河流近域土地利用格局与水质相关性分析——以巢湖流域为例

以河流近域土地利用构成与水环境质量间定量关系为研究对象,基于巢湖流域40个水质监测断面和320个河流近域作用区,运用GIS空间分析功能计算不同尺度作用区内土地利用构成,借助相关分析、冗余分析(RDA)等数理统计手段,分析土地利用构成与水质指数间的相关关系;并分析和比较了流域内山区和平原地区土地利用构成与水质之间关系。结果表明,巢湖流域近河道地区土地利用构成对各水质指数有重要影响,且受地形因素影响。其中,建设用地对河流水质恶化具有明显作用;河流沿岸林地布局能显著改善水质,尤其是对氨氮和高锰酸盐指数敏感;耕地面积变化与水质相关性不显著。RDA冗余分析证实了不同尺度下的土地利用类型对水环境影响具有尺度效应。研究成果对巢湖流域水环境管理具有重要的实际指导意义,并为同类研究提供借鉴     

中国西部矿产资源产业集聚度与竞争力研究

促进矿产资源产业集聚是推动我国西部矿产资源产业发展的主要路径之一,开展我国西部矿产资源产业集聚度与产业竞争力相关性的研究可以为西部矿产资源产业竞争力提升提供相关决策指导.为分析矿产资源产业集聚对矿产资源产业竞争力和区域经济的推动作用,本文选取区位熵反映矿产资源产业集聚度,具体地,选择企业数量区位熵反映矿产资源产业区域集聚度.选取产业产值区位熵反映矿产资源产业经济集聚度;矿产资源产业竞争力则选取产值利润率借以反映.通过衡量我国西部9个矿产资源产业的区域集聚度、经济集聚度和产值利润率,得出我国西部大部分矿产资源产业仍停留于企业"扎堆"的区域集聚,经济集聚不足,尤其是下游加工业,这导致我国西部矿产资源产业集聚效应难以充分发挥,产值利润率低下;进一步的相关性分析表明,西部矿产资源产业集聚度与产业竞争力正相关,且经济集聚对产业竞争力的推动作用更强.因而,不仅要发展下游加工业,而且要提高产业集聚度尤其是经济集聚度,以更有效地推动我国西部矿产资源产业竞争力的提升.     

超补偿理论及其在虫害控制中的意义

本文较为系统地阐述了作物对虫害损伤的超补偿理论,表明超补偿广泛存在于各种作物中,并对超补偿所需条件、基本原因以及与害虫控制的关系作进一步讨论。     

The hybrid model of empirical wavelet transform and relevance vector regression for monthly wind speed prediction

ABSTRACT

In order to improve the prediction ability for the monthly wind speed of RVR, the hybrid model of empirical wavelet transform and relevance vector regression (EWT-RVR) is proposed for monthly wind speed prediction in this study. Compared with empirical mode decomposition (EMD), empirical wavelet transform (EWT) can obtain a more consistent decomposition and have a mathematical theory. In order to testify the superiority of EWT-RVR, several traditional RVR models are used to compare with the proposed EWT-RVR method under the situation of the same embedding dimensions. The experimental results show that the proposed EWT-RVR method has a better prediction ability for monthly wind speed than RVR. It can be concluded that the proposed EWT-RVR method for monthly wind speed is effective.     

Cultural Relevance for Rural Community Development in China: A Case Study in Bai, Jingpo and Huyaodai Communities of Yunnan Province

A three-year study over the Bai, Jingpo and Huayaodai communities in Yunnan Province reveals that the community development is significantly influenced in various ways by such cultural factors as the concepts of development; concepts and traditions of inter-community relationships, consumption, mar- riage and gender; patterns of decision-making and production, resource and income allocation; as well as the role of information dissemination systems, religion and ritual. Based on the analysis over the interactive relevance between each factor and community development, some strategies and methods for dealing with such a cultural relevance in development projects are recommended.     

污水处理厂不同工艺的污泥脱水效能分析及其影响因素研究

污泥脱水是污泥减量的主要手段,直接影响到后续污泥处理处置.本研究以北京某大型污水处理厂A2/O工艺和A2/O-MBR工艺污泥脱水为对象,基于2013年全年的运行数据,分析不同工艺的污泥脱水效果、絮凝剂投配率、污泥脱水电耗和污泥脱水成本,并通过冗余分析(RDA)研究了不同污水处理工艺污泥脱水性能的影响因素.结果表明,污泥脱水性能和絮凝剂投配率均呈现季节性变化特征,冬季污泥较难脱水,絮凝剂消耗大.A2/O-MBR工艺的脱水污泥含水率年均值为(81.92±1.64)%,A2/O工艺为(82.56±1.35)%,污泥脱水絮凝剂消耗(以DS计,下同)分别为(8.70±7.25)kg·t-1和(7.42±2.96)kg·t-1,电耗(以DS计,下同)分别为331.82 k W·h·t-1和121.57 k W·h·t-1.A2/O-MBR工艺的污泥脱水絮凝剂成本(以DS计,下同)为204.76元·t-1,用电成本为231.61元·t-1;A2/O工艺的污泥脱水絮凝剂成本为175.00元·t-1,用电成本为84.86元·t-1.RDA分析表明,水温等季节性因素引起污泥有机质变化是影响污泥脱水性能的关键因素之一,此外,污泥龄也与污泥脱水性能有一定相关性.