Trophoblast-like cells sorted from peripheral maternal blood using flow cytometry: A multiparametric study involving transmission electron microscopy and fetal dna amplification

Three monoclonal antibodies (MAbs) against trophoblast (GB17, GB21, and GB25) and flow cytometry were used to sort trophoblast-like cells (TLCs) from peripheral blood of pregnant women. Sorted TLCs were processed for electron microscopy and fetal DNA amplification of the Y-specific sequences from mothers carrying male fetuses. At the ultra-structural level, most of the nucleated cells had the morphology of leucocytes, suggesting maternal contaminants, and we did not find the characteristic features of the free inter-villous trophoblast cells. Nevertheless, polymerase chain reaction (PCR) analysis showed an amplification of Y-specific sequences in two out of three samples of sorted TLCs. These results suggest that besides the maternal leucocytes, sufficient trophoblast nucleated fetal cells can be obtained using cell enrichment by sorting. This sensitive method holds promise for non-invasive prenatal diagnosis of fetal sex and if sufficient Y(positive) nuclei are found, for the diagnosis of selected numerical chromosome abnormalities.     

A New Modus Operandi for the Agricultural Economics Profession

Agricultural economics has, until the 1990s, enjoyeda reputation for relevance and usefulness to theagri-food industry and policy-makers. Thatreputation has been jeopardized by a growinginfatuation with models and quantification, and aconcomitant underemphasis placed on many complexproblems and issues of society. An illustrativeexample is explored, using agriculturalactivity-related damage to the natural resourcebase, environment and ecology. Agriculturaleconomists are urged to respond by broadening theirterms of reference and joining forces with otherdisciplines.     

旧路改造工程路堤稳定性安全监测方案设计

本文从旧路改造工程的特点出发,通过室内实验获取土力学参数并对沉降量进行预测计算.利用沉降仪、测斜管、孔隙水压力计、土压力盒等多种测试仪器,选取有代表性的观测断面,对经过土工格栅加筋处理的软土地基进行了高路堤施工期间的沉降与稳定观测,并提出适合工程特点的控制标准.从而达到控制路堤稳定、检验填筑质量、控制差异沉降、为路面施工提供技术支持的目的.     

铝合金微电偶腐蚀机理与数值模拟研究进展

综述了铝合金微电偶腐蚀在机理研究和数值模拟两方面的研究进展,针对独立微电偶的腐蚀机理表征、多元微电偶空间随机分布相互作用规律、结构/载荷因素与铝合金微电偶腐蚀驱动力间的竞争机制等3个方面进行了展望,期望为铝合金微电偶腐蚀研究提供方向参考。     

湿地植物在城镇污水处理系统中的作用特性研究

将不同类型湿地植物如凤眼莲、荇菜、稗草、黑藻、皇竹草、美人蕉等,引入城镇污水处理系统,考察其对污染物的去除效果和在湿地组合系统中的作用特性。盆栽实验发现,虽然植物对污水N、P营养元素的直接去除率分别只有14.0%～59.4%和8.6%～55.2%,但植物通过过滤、截留及加强有机物降解转化的作用,对水体中污染物的去除具有协同效应,使水体N、P的总去除率分别达65.4%～83.8%和43.5%～69.5%。将植物引入组合处理系统,中试实验结果表明,水体TN、TP和COD月平均去除率分别高达80.4%、74.3%和81.3%。与常规生化处理系统相比,处理效果大为增强,生化反应的能耗相对减少,出水水质较稳定,使湿地组合系统成为一种去N除P,防止水体富营养化的有效处理方法。     

Quantifying the squeezing or stretching of fisheries as they adapt to displacement by marine reserves

The designation of no‐take marine reserves involves social and economic concerns due to the resulting displacement of fishing effort, when fishing rights are removed from those who traditionally fished within an area. Displacement can influence the functioning of the fishery and success of the reserve, yet levels of displacement are seldom quantified after reserve implementation and very rarely before that. We devised a simple analytical framework based on set theory to facilitate reserve placement. Implementation of the framework requires maps of fishing grounds, fishing effort, or catch per unit effort for at least 2 years. The framework quantifies the level of conflict that a reserve designation might cause in the fishing sector due to displacement and the opportunities to offset the conflict through fisher spatial mobility (i.e., ability of fishers to fish elsewhere). We also considered how the outputs of the framework can be used to identify targeted management interventions for each fishery. We applied the method in Honduras, where the largest marine protected area in Central America is being placed, for which spatial data on fishing effort were available for 6 fisheries over 3 years. The proposed closure had a greater negative impact on the shrimp and lobster scuba fisheries, which concentrated respectively 28% and 18% of their effort inside the reserve. These fisheries could not accommodate the displacement within existing fishing grounds. Both would be forced to stretch into new fishing grounds, which are available but are of unknown quality. These stakeholders will likely require compensation to offset costly exploratory fishing or to travel to fishing grounds farther away from port.     

基于ANFIS模型的基坑工程位移反分析

在软土地基深基坑开挖支护工程的设计与施工中,岩土体力学参数的取值问题是关键。基于MATLAB(R2007a)模糊工具箱中的自适应神经模糊推理系统(ANFIS)对基坑岩土体力学参数进行了位移反演分析,并结合汉口火车站出站厅基坑工程实例,动态反演了具体参数,检验了反演精度,从而验证了该方法用于基坑工程位移反分析的可行性及工程适用性。     

多热源分散程度的数学模拟及对置换通风影响

通过建立多污染热源置换通风的三维数学模型,根据数值解分析三热源位置变化对多污染源置换通风效果的影响,结果表明:热源的位置变化对多污染热源置换通风效果的影响是很大的,房间内热源应紧凑布置以利于提高通风效果和节能。     

降低循环冷却水水耗的生产措施

为了有效降低循环冷却水系统补充水量、排污量,节约水处理剂的消耗、降低冷却水处理成本,关键在于提高循环水的浓缩倍数。本文对影响循环水浓缩倍数的因素进行了分析,总结了哈尔滨石化公司循环水场存在的问题。同时本文给出了提高循环水浓缩倍数的方法,上述方法能取得显著的环境效益和经济效益。     

西安f7地裂缝发展变化的预测研究

论文首先分析和总结了影响西安地裂缝发展变化的主要人为因素。随后,利用三维非稳定渗流分析方法研究了人类活动对地下水位的影响;根据西安市新增固定资产投资的变化,预测了地面荷载的变化;以时间、地下水位和地面荷载为基本变量,建立了预测f 7地裂缝活动趋势的BP神经网络模型;最后,借助MATLAB语言进行编程,利用训练稳定的网络模型,预测了f 7地裂缝的活动趋势以及年平均的垂直位移沉降量,为进一步研究西安地铁2号线安全运行和防护提供参考依据。