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41.
家蝇抗菌蛋白的分离纯化及部分性质 总被引:14,自引:0,他引:14
通过体壁损伤诱导家蝇(Musca domestica)幼虫产生免疫血淋巴,经沸水浴热变性、减压蒸馏浓缩、Sephadex G-15柱脱盐、CM-Sepharose离子交换层析、Sephadex G-50凝胶过滤等步骤,分离得到一种具抗菌活性的蛋白质,并达电泳纯,结果表明,分离物不具血细胞凝集活性,也不是溶菌酶,而是一种未见报道的抗菌蛋白,经SDS-PAGE测得,该抗菌蛋白Mr=12600,等电点为9.8。 相似文献
42.
Kim KY Ko HJ Kim HT Kim YS Roh YM Lee CM Kim CN 《Environmental monitoring and assessment》2007,133(1-3):255-266
This on-site survey study was performed to determine the concentrations and emissions of aerial contaminants in the different
types of swine houses in Korea and then to present beneficial information available for Korean pig producers to manage optimal
air quality in swine house. The swine houses investigated in this research were selected based on three criteria; manure removal
system, ventilation mode and growth stage of swine. Mean concentrations of aerial pollutants in swine houses were 8 ppm for
ammonia, 300 ppb for hydrogen sulfide, 2 mg m−3 for total dust, 0.6 mg m−3 for respirable dust, 4 log(cfu m−3) for total airborne bacteria and 3 log(cfu m−3) for total airborne fungi, respectively. Mean emissions based on pig (liveweight; 75 kg) and area (m2) were 250 and 340 mg h−1 for ammonia, 40 and 50 mg h−1 for hydrogen sulfide, 40 and 50 mg h−1 for total dust, 10 and 15 mg h−1 for respirable dust, 1.0 and 1.3 log(cfu) h−1 for total airborne bacteria and 0.7 and 1.0 log(cfu) h−1 for total airborne fungi, respectively. In general concentrations and emissions of gases were relatively higher in the swine
houses managed with deep-pit manure system with slats and mechanical ventilation mode than the different swine housing types
whereas those of particulates and bioaerosol were highest in the naturally ventilated swine houses with deep-litter bed system. 相似文献
43.
44.
Nickel oxide nanoparticles decorated carbon nanotube nanocomposites(Ni O·CNT NCs)were prepared in a basic medium by using facile wet-chemical routes. The optical,morphological, and structural properties of Ni O·CNT NCs were characterized using Fourier transformed infra-red(FT-IR), Ultra-violet visible(UV/Vis) spectroscopy, field-emission scanning electron microscopy(FESEM), X-ray energy dispersed spectroscopy(XEDS), X-ray photoelectron spectroscopy(XPS), and powder X-ray diffraction(XRD) methods. Selective4-aminophenol(4-AP) chemical sensor was developed by a flat glassy carbon electrode(GCE, surface area: 0.0316 cm~2) fabricated with a thin-layer of NCs. Electrochemical responses including higher sensitivity, large dynamic range(LDR), limit of detection(LOD), and long-term stability towards 4-AP were obtained using the fabricated chemical sensors. The calibration curve was found linear(R~2= 0.914) over a wide range of 4-AP concentration(0.1 nmol/L–0.1 mol/L). In perspective of slope(2 × 10~(-5)μA/μM), LOD and sensitivity were calculated as 15.0 ± 0.1 pM and ~ 6.33 × 10~(-4)μA/(μM·cm) respectively. The synthesized Ni O·CNT NCs using a wet-chemical method is a significant route for the development of ultrasensitive and selective phenolic sensor based on nano-materials for environmental toxic substances. It is suggested that a pioneer and selective development of 4-AP sensitive sensor using Ni O·CNT NCs by a facile and reliable current vs voltage(I–V)method for the major application of toxic agents in biological, green environmental, and health-care fields in near future. 相似文献
45.
四川地震后,大量房屋倒塌,产生了大量的建筑垃圾,对生态环境造成严重影响,同时使得大量灾民无家可归。文章从节约成本、保护生态环境、走可持续发展道路考虑,提出用再生混凝土建生态友好型安置房的思路,并给出了建生态友好型安置房的几点建议。 相似文献
46.
北京市民居室内气态多环芳烃呼吸暴露评价 总被引:2,自引:0,他引:2
采用被动采样装置采集并测定北京地区38个家庭采暖期和非采暖期室内空气中的7种气态多环芳烃(PAHs)的含量.结果表明,室内气态PAHs以2、3环组分为主,7种气态PAHs的总平均浓度为100 ng/m3左右.根据实测的PAHs浓度、不同年龄段人群室内平均停留时间及其相应的呼吸速率,计算得到室内成年人(包括老人)对7种气态PAHs的潜在总暴露速率在采暖期和非采暖期分别为66 ng/h和58 ng/h;而未成年人的潜在总暴露速率则分别为56 ng/h和50 ng/h. 相似文献
47.
48.
链篦机一回转窑法氧化球团工艺是国外较为成熟,国内较为先进的球团生产工艺,其抽风干燥烟气属高温、高湿、高含硫量烟气。对净化抽风干燥烟气采取的主要技术措施进行了介绍,可供类似工程实践参考。 相似文献
49.
Air quality of gaseous emissions from crude petroleum flames and fumes were monitored in a poultry house at 10 min intervals; from 10.10am–11.10am daily for 14 days. The crude petroleum burning was simulated in a metal burner, 22.86 cm high with a diameter of 17.80 cm and a thickness of 1.27 cm designed for the purpose. The micro-climate (ambient temperature, relative humidity and light intensity) of the experimental environment (poultry houses) was recorded. Results showed that the mean gaseous pollutants emitted before and during the experiments varied within the poultry house. Gases such as sulphur dioxide (SO2), hydrogen sulphide (H2S), methane(CH4) and carbon monoxide (CO) were higher in the brooding than finishing phase, while volatile organic carbon (VOC) and suspended particulate matter (SPM) levels were lower at the brooding than finishing phase. The values obtained in this study for 60 min (at 10 min intervals) are far higher than the values elucidated by the Federal Environmental Protection Agency, which are outdoor air quality measurements as compared to those in this study measured indoors. 相似文献
50.