真鲷去毒相关基因cDNA序列的克隆与肝脏组成型表达分析

赤潮发生时产生的一些海洋生物毒素对人类和海洋动物的安全形成潜在的威胁甚至导致死亡.为从分子水平探讨鱼类中海洋藻毒素的去毒分子机理,采用RT-PCR法克隆了真鲷(Pagrus major)肝脏芳香烃受体核转位蛋白(ARNT)和I时相异生素代谢酶细胞色素P4501A(CYP1A)基因cDNA核心序列,同时,应用半定量RT-PCR方法,以β-肌动蛋白作为外参照,在其指数期增长的范围内研究了芳香烃受体(AHR)、ARNT、CYP1A、II时相异生素代谢酶alpha型谷胱甘肽S-转移酶(GSTA1、GSTA2)、rho型谷胱甘肽S-转移酶(GSTR)、热休克蛋白70(HSP70)基因组成型表达水平.结果发现,真鲷ARNT、CYP1A基因cDNA核心序列片段分别长438bp和908bp,分别编码146和302个氨基酸.序列同源性分析发现,真鲷与门齿鲷(Stenotomus chrysops)、石首鱼(Micropogon undulatus)ARNT基因氨基酸序列同源性高达97.2%、95.2%,与斑马鱼(Brachydanio rerio)、人、大鼠、小鼠ARNT同源性较低(77.2%～79.3%).真鲷与门齿鲷、金头鲷(Sparus auratus)、欧洲川鲽(Rhombus maximus)、欧洲海鲈(Dicentrachus labrax)CYP1A基因氨基酸序列同源性较高,为84.8%～94.0%,与斑马鱼、人、小鼠CYP1A同源性较低,为59.6%～77.8%.真鲷肝脏AHR、ARNT、CYP1A、GSTA1、GSTA2、GSTR和HSP70基因组成型表达水平分别为(25.32±6.56)%、(26.22±4.24)%、(146.5±16.06)%、(55.42±3.75)%、(48.82±10.89)%、(79.47±3.13)%、(107.42±14.34)%.     

资源型城市的“无废城市”建设模式探讨

资源型城市在我国经济发展大局中地位重要而特殊、历史贡献突出,城市发展的生命周期特征和物质代谢的"大进大出"特征明显,固体废物(以下简称固废)治理问题积弊已久、复杂多变。如何建设"无废城市"模式的资源型城市,是当前城市可持续发展和"无废城市"实践亟待破解的难题。本文研究分析了2017年我国资源型城市固废产生、贮存、综合利用、处置环节及管理制度体系建设的主要问题,并从制度设计、产业转型、分类利用、标准建立四个方面提出了"无废"资源型城市建设的政策建议。根据资源型城市所处生命周期发展阶段的不同,提出了基于固废"产、存、用、置、销"的存量、流量挂钩约束的发展模式。同时提出以下建议:快速成长期城市应从产业体系构建、空间布局及准入条件着手控制固废增量;平稳成熟期城市应发展完善高值化资源综合利用产业体系和制度体系;枯竭衰退期城市应注重挖掘和开发存量固废的资源价值;再生发展期城市应加强多源废物协同处置利用产业体系建设。本研究可为资源型城市的"无废城市"建设提供模式参考和政策建议。     

基于矿渣的一种新型建筑材料试验研究

通过化学分析、抗压强度、抗折强度等试验,研究了一种以矿渣为主要成分的新型建筑材料。试验结果表明：该种新型建筑材料的抗压强度和抗折强度能够达到525水泥相关要求,可满足实际工程的需要。     

Willing and able: explaining individuals' engagement in environmental policy making

Research on attitudes toward environmental policy making has often overlooked the important role that risks play in forming individual attitudes towards the environment. Similarly, this research often assumes that the factors that affect attitudes are similar across different environmental domains. The purpose of this research is to examine how the factors that affect an individual's willingness to get involved in environmental policy making differ on two environmental issues – nuclear power and drinking water. The study utilises multivariate statistical techniques to explore the relationship that uncertainty, risk, and trust play in an individual's willingness to take action in environmental policy making. The data consist of responses to a national random telephone survey of 403 adults in the United States. The individuals surveyed are more likely to take action on the less avoidable potential threat of nuclear power than they are on drinking water. The respondents believe that the potential harm from nuclear power is greater than that from drinking water. The individuals most likely to take action are those who indicate that they are interested in environmental issues.     

Potential utilization of riverbed sediments by hydrothermal solidification and its hardening mechanism

Hydrothermal solidification of riverbed sediments (silt) has been carried out in a Teflon (PTFE) lined stainless steel hydrothermal apparatus, under saturated steam pressure at 343–473 K for 2–24 h by calcium hydrate introduction. Tobermorite was shown to be the most important strength-producing constituent of the solidified silt. A longer curing time or a higher curing temperature was shown to be favorable to the tobermorite formation, thus promoting strength development; however, overlong curing time (24 h) seemed to affect the strength development negatively. The hardening mechanism consisted of the crystal growth/morphology evolution during the hydrothermal process. The species dissolved from the silt were precipitated first as fine particles, and then some of the particles seemed to build up the rudimental morphology of calcium silicate hydrate (CSH) gel. The CSH gel, with precipitated particles, appeared to cause some reorganization within the matrix, which made the matrix denser and thus gave an initial strength development. Tobermorite, transformed inevitably from the CSH gel, reinforced the matrix with its interlocked structure, and thus further promoted the strength development.     

Sustainable design of sanitation system based on material and value flow analysis for urban slum in Indonesia

Material flow analysis (MFA) and value flow analysis (VFA) were applied to the sanitation system in an urban slum in Indonesia. Based on the results of the MFA and VFA, garbage and excreta disposal costs were evaluated to be 0.7% and 1.1%, respectively, of per capita income. Such value flows seem reasonable in light of the recognized affordability to pay (ATP) standard. However, current excreta disposal methods create negative impacts on downstream populations. Because such disadvantages do not go back to disposers, but passed to downstream, the current value flow structure does not motivate individual toilet users to install treatment facility. Based on current material and value flow structures, a resource recycling sanitation system scenario was examined. Based on VFA, an affordable initial cost for such a system was calculated; this was found to be comparable in price to a cheaper composting toilet that is currently available in the market.     

EPR核电厂核岛厂房分层分解法防火分区设计

防火分区设计是实现防火安全目标最核心、最关键的环节,是开展防火安全评价的基础。在台山一期的核岛防火设计应用中,出现防火小区定义与标准不一致、防火区与防火小区的嵌套关系混乱、各防火分区类型的应用原则不清晰等不足。基于自主化设计的需要,通过整理分析台山一期核电厂(EPR堆型)核岛防火分区设计成果,深层研究思考EPR设计理念,进一步理清不同分区类型之间的嵌套逻辑,建立了一套分层分解的核岛防火分区设计方法——以分区嵌套顺序进行分层、以功能识别为导向进行区域分解,从而化繁为简,实现分区设计的流程化。该设计方法很好地弥补了EPR在此方面的不足,为后续自主化设计及新堆型的开发提供了重要的参考。     

基于多需求点的震灾应急物资调度模型研究

为了解决震后应急物资调度中,因不考虑各应急需求点灾情差异和需求差异带来的决策结果不够合理的问题,采用改进的专家打分法将收集到的灾情信息量化为灾情因子,在此基础上建立应急成本和应急延误时间的多目标模型。采用逐步法求解,为每一个应急需求点确定提供应急物资的供应点及供应量。将此方法运用到云南发生的某地震灾后救援中,计算得出与3个应急需求点对应的应急供应点及其供应量。     

防尘口罩结构/材料及其加工技术探讨

介绍了防尘口罩的结构及选用时应注意的一些问题,分析了作为防尘口罩核心组件的滤料的过滤机制。论述了一些非织造布材料和非织造布加工方式对于防尘口罩滤料生产的优点和不足之处。对于广泛应用于口罩滤料的聚丙烯材料及滤料的主要加工方式——熔喷法非织造布生产技术,进行了重点介绍。同时也简要介绍了为完善口罩的综合性能而采用的非织造布复合技术。最后研究分析了提高口罩过滤效率的一种非常重要的方法——非织造布驻极加工方法,并对目前防尘口罩应用中存在的问题进行了简要的分析。     

Ignition of dust-air atmospheres by ultrasonic waves

This paper shows the results of our investigations on the ignition source ultrasound in dust-air atmospheres. Ultrasound is, on the one hand, considered to be an ignition source according to international safety standards (EN 1127–1 (2011)). On the other hand, though, ultrasound is used for various applications in gases and air, such as level and flow measurement, or in the process industry, but no explosion accidents have yet been reported. Our research now shows that it is indeed possible to ignite dust-air mixtures in ultrasound fields under certain conditions. We conducted our experiments in an ultrasound standing wave field and used maize starch, calcium stearate and sulfur dust. For ignitions, an absorbing target was needed to convert the acoustic energy into heat. From theoretical estimations and experiments critical conditions that provoke ultrasonically triggered explosions are identified.