西双版纳自然环境试验站环境真菌的鉴定

采用自然沉降法对西双版纳自然环境试验站的环境真菌进行收集和分离;同时采用显微形态观察对其中的16株真菌进行分类鉴定,结果16株真菌归属于2个纲、4个目、5个科、10个属;结合ITS区间序列分析,确定5个主要环境真菌类群的分类地位。本研究将有助于开展环境试验和防霉研究工作。     

攀钢炼钢厂除尘技术改造思路及效果

就攀枝花新钢钒提钒炼钢厂转炉作业区转炉一次除尘系统、转炉二次除尘系统及脱硫扒渣烟气除尘系统在几年的运行中分别存在的问题进行了分析,主要包括由于炼钢工艺变化引起的除尘系统能力不足,原设计参数不太合理及原系统中设备配置不足等,对于以上问题提出了相应的改进思路并实施了相应的除尘改造,改造以后取得了较好预期效果。     

环戊烯臭氧化反应瞬变物种产生机制

大气中烯烃臭氧化反应产物是二次有机气溶胶(SOA)形成的重要前体物. 运用自行组建的低温基质隔离系统研究环戊烯臭氧化反应的瞬变物种产生机制. 采用程序升温方法,将温度从6 K升至115 K,用傅里叶红外光谱仪检测不同温度下生成的活性中间体,并将试验值与理论计算值、文献报道值进行对比,以确定中间体构型. 结果表明:环戊烯臭氧化反应生成的中间体——POZ(初级臭氧化物)、SOZ(二次臭氧化物)和CI(一分子羰基氧化物和一分子醛或酮)被成功检测到. 其中,POZ的特征吸收峰在705和946 cm-1处,对应O—O—O反对称伸缩振动和环变形振动;SOZ特征吸收峰在939 cm-1处,对应C—O伸缩振动;CI的特征吸收峰在1 733和853 cm-1处,分别对应CO伸缩振动和O—O伸缩振动. 据此,环戊烯臭氧化反应遵循Criegee机制. 采用4种量子化学计算方法——B3LYP/6-311++G(d,2p)、B3LYP/6-311++G(3df,3pd)、MP2/aug-cc-pvdz和PW91PW91/6-311++G(3df,3pd),计算POZ、SOZ和CI特征吸收峰的振动频率,其中POZ在705 cm-1处吸收峰的振动频率计算值分别为725、698、703、680 cm-1;SOZ在1 030 cm-1处吸收峰的振动频率计算值分别为1 017、1 018、1 025和993 cm-1;CI在1 733 cm-1处吸收峰的振动频率计算值分别为1 796、1 803、1 732和1 745 cm-1.表明MP2/aug-cc-pvdz计算的特征振动频率与试验值最接近.      

甘肃党参中蛋白质的提取分析

实验采用盐溶法提取甘肃党参中蛋白质,用饱和(NH4) 2SO4溶液进行分级盐析、透析、浓缩后用SDS- PAGE分析,测定不同提取液中主要蛋白质的分子量.结果表明,用pH为6.0的PB缓冲液提取并用饱和度在60％以上的(NH4)2SO4溶液盐析时蛋白提取效果最好;经SDS- PAGE电泳后,初步鉴定出所提取党参蛋白质亚基中最大分子量为68885Da,最小分子量为15570Da.     

浅谈电梯导轨隔振降噪的实践研究

现代高层住宅建筑中,电梯井道与住户分隔墙体常为同一实体,使得电梯高速运行时会有较大的导轨振动噪声影响与井道共墙的相邻住户。本文论述了电梯导轨噪声的产生机理、相关技术进展,分析了部分降噪治理技术误区,并结合导轨隔振降噪治理的成功案例分析,提出正确的电梯导轨噪声治理方法。     

近20a三峡库区农林地利用变化图谱特征分析

土地利用变化图谱单元能够把表示"空间单元特征的图"与表示"时间发展之起点与过程的谱"合为一体,并建立起空间—属性—过程一体化数据信息。论文以TM/ETM影像为空间数据源,构建三峡库区1990—2000年和2000—2010年两个阶段农林地利用变化信息图谱,以及融合1990、2000、2010年这三个时期土地利用现状的变化模式图谱,以探讨库区近20 a来的农林用地变化轨迹和特征规律。结果表明:1 1990—2000年阶段的农林地利用变化图谱主要以农林地间及其和草地互换、建设用地占用农林地的图谱单元类型为主。空间差异上最为显著的是"耕地→建设用地(编码15)"、"草地→林地(编码32)"和"林地→草地(编码23)",空间分离度较低。2 2000—2010年阶段的农林地利用变化图谱单元分离度增大,农林地利用发生转换空间进一步扩张。最显著变化的是"耕地→林地(编码12)"和"耕地→建设用地(编码15)"图谱单元类型,其次是农林地转换为水域图谱类型(编码14和24)的面积增大。3库区农林地利用变化模式以前期变化型和后期变化型为主,反复变化型和持续变化型发生概率较小,库区农林地利用的转换具有明显的时间阶段性特征。     

Microsatellite analysis provides efficient confirmation of fetal trophoblast isolation from maternal circulation

Fetal trophoblasts can be found in maternal circulation from an early stage of pregnancy and thus provide a potential source of DNA for non-invasive prenatal diagnosis. We have developed a two-step method for trophoblast isolation between the 8th and 12th week of pregnancy. Blood was sampled from 14 women undergoing termination of pregnancy or spontaneous abortion. Immunomagnetic beads precoated with HLA class I and II, and with anti-cytokeratin-18 monoclonal antibodies, were used to remove CD8+ and other maternal cells, and to select for fetal trophoblasts, respectively. Microsatellite analysis was performed on DNA extracted from the isolated, maternal, paternal and placental cells after PCR amplification. Recovery of the trophoblasts was confirmed in 13/14 cases (93%) by the identification of an identical microsatellite pattern for fetal and placental cells. Further evidence was the presence of heterozygous alleles of both maternal and paternal origin. The correct prediction of gender in all five male fetuses was an additional confirmation of trophoblast recovery. We conclude that trophoblasts can be effectively isolated from maternal blood in the first trimester, and by using polymorphic microsatellite markers to confirm sample purity, this method has potential future application in prenatal diagnosis. Copyright © 2001 John Wiley & Sons, Ltd.     

辽东湾海冰中微生物的分离

本文报道了辽东湾４个采样点海冰样品中异差细菌异养真菌、粪大菌群等微生物的分离，并用海水和淡水培养基分别对其进行了菌数分离比较，同时还对其中两个采样的冰下水与沉积物样品做了分离对照。结果表明，海冰（化冰水）样品中异养细菌的丰度为：１０＾３－１０＾５ＣＦＵ／ｄｍ＾３，异养真菌为：１０＾１－１０＾２ＣＦＵ／ｄｍ＾３；弧菌为；〈１０＾２－１０＾３ＣＦＵ／ｄｍ＾３；石油烃降解菌为：１０＾２－１０＾４ＣＦＵ／     

在较高位置构筑大型隔声屏的降噪工程

在控制工厂二层楼顶的风机群及冷却塔群的噪声工程中，复式隔声屏获１８ｄＢ（Ａ）的降噪效果。主屏由轻结构板及隔振底盘组成。主习体被若干伸缩隔断以消除热应力。副屏是为了减少主屏隔振底盘缝的漏声。迷路消声构造减少伸缩缝的漏声。为了抗风，主习必须锚固，并且屏体自身应有良好的结构。     

Testing the ability of males and females to respond to altered songs in the dueting green lacewing, Chrysoperla plorabunda (Neuroptera: Chrysopidae)

Green lacewings in the carnea group of Chrysoperla engage in species-specific heterosexual duets using low-frequency substrate-borne signals. Within each species, both sexes sing nearly identical songs. Songs are the principal barriers to hybridization between sympatric species in the complex. Here, we investigated the responsiveness of males and females of Chrysoperla plorabunda to synthesized, prerecorded songs that differed from the species mean in the period between repeated volleys of abdominal vibration. We tested 15–16 males and 15–16 females using playbacks of two signals that gradually increased or decreased in volley period, starting at the species mean. We found that (1) duets during courtship are accurate, interactive, and adjustable by each participant; (2) in staged duets, both sexes respond best to song tempos near the mean volley period of their population, but can nonetheless maintain duets with signals of nearly twice, or half, the normal volley period; (3) individuals fine-tune their adjustments to signals of different volley periods by changing their own volley duration and latent period, or less often by inserting extra volleys or skipping every other volley; (4) males are significantly better at matching signals of changing tempo than females; and (5) the range of song responsiveness of C. plorabunda does not overlap the natural range of volley periods found in Chrysoperla adamsi, an acoustically similar sibling species, thus reaffirming strong behavioral isolation. In sum, the precise, almost unbreakable heterosexual duets characteristic of song species of the carnea group result from tight mutual feedback between partners. Effective reproductive isolation between species can be based on song differences alone.