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Using the polymerase chain reaction (PCR), it was possible to amplify a single copy fragment of the β-globin gene from 2–32 human embryonic cells obtained from arrested preimplantation embryos. For the detection of β-thalassaemia mutations, allele specific priming of the PCR using nested primers was employed using approximately 10 pg of DN A from individuals known to carry these mutations. This approach was successful in detecting the presence or absence of five Asian Indian β-thalassaemia mutations that were selected for this study. In spite of meticulous precautions against contamination, false-positive amplification was observed, a problem that will have to be overcome before this approach can be used in clinical practice. 相似文献
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双酚A(BPA)作为一种典型的环境内分泌干扰物,与二型糖尿病和肥胖症等代谢类疾病密切相关.在这项研究中,旨在探讨BPA直接暴露以及围产期暴露对本体和子代胰岛β细胞影响的差异,从而实现对不同易感人群的精准预防与治疗.通过对GEO数据库中GSE126297和GSE82175两个数据集进行分析,共筛选出108个共有的差异基因,其中28个表达趋势一致,而另外80个表达趋势相反.随后对本体和子代单独的差异基因,以及共有差异基因进行了GO和KEGG对比分析.此外,利用String数据库和Cytoscape分析PPI网络中的紧密联系的基因,并将其可视化,进一步从28个表达趋势一致的基因中筛选出相互作用最为紧密的基因集1(Iqgap2,Igf2r和Rab18);从80个表达趋势相反的基因中筛选出相互作用紧密的基因集2(Polr2f,Ccnt2,Polr2e,Kdm7a,Fbl)和基因集3(Atp5g2,Atp5o和Ndufa6).最后,通过GEPIA在线验证上述基因在胰腺癌样本中的表达,研究发现Rab18,Polr2f,Polr2e,Fbl,Atp5g2,Atp5o和Ndufa6在胰腺癌病例中的表达均显著上升.同时,上述基因在本体中呈现显著上升趋势,而对于子代,只有Rab18显著上升,其他基因则呈下降趋势.综上,BPA对本体和子代的影响有一定的关联,其中关联的关键基因的异常表达可能会增加本体罹患胰腺癌的风险,而对于子代则会影响代谢过程和细胞结构. 相似文献
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Qing Xia Deyang Kong Guoqiang Liu Qingguo Huang Aamr Alalewi Junhe Lu 《Frontiers of Environmental Science & Engineering》2014,8(3):372-378
The removal of 17β-estradiol (E2) in laccase catalyzed oxidative coupling processes was systematically studied in this work. We focused on the influence of pH and natural organic matter (NOM) on the performance of the enzymatic treatment processes. It was found that the optimal pH for E2 removal was between 4 and 6. The removal of E2 was slightly inhibited in the presence of NOM. Enzymatic transformation of E2 was second-order in kinetics with first-order to both the concentrations of the enzyme and contaminant. Mass spectrum (MS) analysis suggested that coupling products were formed through radical-radical coupling mechanism. The results of this study demonstrated that laccase catalyzed oxidative coupling process could potentially serve as a treatment strategy to control steroid estrogens. 相似文献
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Laboratory studies were conducted to determine the adsorption/desorption equilibrium of carbofuran between four divergently textured soils and distilled water and an aqueous solution of 0.01?mol?L?1 β-cyclodextrin using a batch equilibrium technique. The determined adsorption isotherms for silt loam soils were of L-shaped, for sandy loam soil S-shaped, in agreement with a Freundlich isotherm. In the presence of β-cyclodextrin, the adsorption of carbofuran to the four soils was lower than with distilled water. Positive hysteresis was observed in all soils with distilled water, negative hysteresis when using a solution of β-cyclodextrin as desorbent. The results indicate the potential use of β-cyclodextrin for remediation of pesticide-contaminated soils. 相似文献
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Shifen Xu Elisa D’Angelo Dhritiman Ghosh Jessika Feliciano Sapna K Deo Sylvia Daunert 《毒物与环境化学》2013,95(3):287-298
Polychlorinated biphenyls (PCBs) are a mixture of 209 individual chlorinated compounds commonly known as PCB congeners. These compounds are hydrophobic and are persistent in the environment. Their use was banned in the US a few decades ago because of harmful health effects. Therefore, detection of PCBs in environmental samples is increasingly important. To that end, we have developed a two-step simple and sensitive method for the detection of total PCBs. Specifically, our method involves dechlorination of PCBs to biphenyl followed by detection of biphenyl using a whole cell sensing system as the detection system. The whole cell sensing system consists of cells of the strain Pseudomonas pseudoalcaligenes KF707 harboring plasmid pSD7000. Plasmid pSD7000 contains the gene of lacZ, a reporter protein under the control of the bph operon. The detection is achieved through the emission of light afforded by the expression of reporter protein triggered by the presence of biphenyl. Due to the fact that this operon is activated only by few PCB congeners, a chemical dechlorination method was employed to convert all PCBs to biphenyl, and thus all the PCBs present in a given sample are able to be detected. The results showed that PCB congeners were rapidly (30?min) and efficiently (>98.5%) dechlorinated to biphenyl using a Mg/K2PdCl6 catalyst, and the biphenyl could be subsequently quantified using the whole cell sensing system. This hybrid analytical method that combines classical dechlorination with novel biosensing methods may find applications in the on-site monitoring of PCBs contamination levels. 相似文献
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污灌与镉胁迫对菠菜几种抗氧化酶活性的影响(Effects of Sewage Irrigation and Cadmium Stresses on the Activities of Several Antioxidant Enzymes of Spinach) 总被引:1,自引:0,他引:1
为了探讨污水灌溉与镉胁迫对菠菜抗氧化酶活性的影响,采用盆栽试验的方法,分别用清水和生活污水配制不同浓度(0、1、5、10、50mg·L-1)的Cd2+溶液,对菠菜进行受试处理,每5d灌喷1次,共处理5次.分别采用邻苯二酚比色法、L-苯丙氨酸比色法、水杨酸比色法和高锰酸钾滴定法,对菠菜叶片多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)、β-1,3葡聚糖酶(β-1,3glucanase)和过氧化氢酶(CAT)活性进行了测定.结果表明:1)清水处理系列:随着镉处理浓度的增大,与清水对照相比,PPO、PAL活性呈先升高后降低的趋势,CAT活性略有升高,β-1,3葡聚糖酶活性略有降低,各暴露组与清水对照组相比,均呈现显著差异(p<0.05).2)污水处理系列:随着镉处理浓度的增大,与污水对照相比,PPO、PAL活性呈先升高后降低的趋势,CAT和β-1,3葡聚糖酶活性呈逐渐降低趋势;各暴露组与污水对照相比,均呈现显著差异(p<0.05).3)相同Cd2+浓度下,与清水处理相比,各浓度污水处理PAL和PPO活性均明显降低(p<0.05);而β-1,3葡聚糖酶明显升高(p<0.05).以上结果显示:单一镉污染和生活污水与镉复合污染对菠菜不同抗氧化系统酶具有不同影响,这可能与污水中各成分与镉的相互作用有关. 相似文献