摇蚊乙酰胆碱酯酶最佳反应体系的建立及有机磷类药剂敏感度比较

花翅摇蚊(Chironomus kiiensis)是一种重要的水生昆虫,经常用于水体环境质量的生物学评价.用正交试验方法研究了酶浓度、底物浓度、反应体系pH值、反应温度、反应时间5个因素对花翅摇蚊(Chironomus kiiensis)乙酰胆碱酯酶(AChE)活性测定的影响.通过对正交试验结果进行极差和方差分析,明确了测定摇蚊AChE活性的最适反应条件是酶浓度为5头/mL、底物浓度为6 mmol/L、pH值为8.0、温度为35℃、反应时间为10 min.DDVP、马拉硫磷和甲胺磷对花翅摇蚊的LC50值分别为8.93 mg/L、16.74 mg/L和47.65 mg/L.利用正交试验得到的反应最适条件进一步研究了花翅摇蚊接触上述3种药剂亚致死剂量后对其体内AChE活性的影响.结果表明,无论是时间效应还是剂量效应,3种药剂对花翅摇蚊幼虫体内AChE影响的趋势与生物测定结果是一致的,其对花翅摇蚊幼虫体内AChE的抑制能力依次为DDVP、马拉硫磷和甲胺磷.     

Low level inhalation experiments with four different cadmium compounds in rats†

This long‐term inhalation study was designed to describe the toxicity and the carcinogenic risk from Cd compounds because it had been shown from former long‐term inhalation studies that cadmium choloride induced primary lung tumors in Wistar rats. It was therefore logical to examine whether other cadmium compounds to which human beings are more frequently exposed have also carcinogenic potency. In a long‐term inhalation study cadmium aerosols consisting of cadmium chloride (CdCl₂), cadmium oxide (CdO) as dusts and fumes, cadmium sulfate (CdSO₄), cadmium sulfide (CdS) and a combination of cadmium oxide/zinc oxide were used. Wistar rats were continuously exposed in inhalation chambers for 18 months 22 hrs a day or for 40 hrs a week. The studies will be terminated at the mean survival life time of the species. The aerosols were generated by several different systems. The particles of the cadmium aerosols have the average mass medium diameters in the range from 0.2 to 0.5 μm.     

Urban riverbank residents' evaluation of flood evacuation policies in Winnipeg,Manitoba, Canada

The results of a discrete choice experiment (DCE) as a part of a survey among the urban riverbank residents on the Red River in Winnipeg, Manitoba, Canada, indicated that the risk of over-dike flooding, set at 2ft above the 1997 flood water level, was a significant determinant of both voluntary and mandatory evacuation, compared to those set at the 1996 or 1997 levels. Mandatory evacuation was more preferred over voluntary evacuation when the likelihood of flooding was at its most severe, and the opposite relationship was the case when the likelihood was low. The notification time for evacuation, suggested as 1, 2, and 4 days, proved to be an insignificant attribute, whereas the respondents indicated significant preference for full flood compensation over an offer of either 80% or 90% flood relief, irrespective of the alternatives of voluntary or mandatory evacuation.     

The uptake and translocation of selected elements by cole (Brassica) grown using oasis soils in pot experiments

Pot experiments were conducted on cole (Brassica) grown in soils jointly treated with traces of two heavy metals cadmium (Cd) and zinc (Zn). As the concentration of heavy metals in the soil increased, the uptake of these metals by the plants rose. However, the ratio of heavy metal concentration in soil to uptake by plants increased at a slower rate. Bioavailability of heavy metals considered between the roots and soil using non-linear regressions was shown to be statistically significant. Similarly, the bioavailability of these two heavy metals between leaves and roots using a linear regression was also statistically significant. The bioconcentration factors (BCFs) for Cd and Zn were 0.282 and 4.289, respectively. Significant variation of BCF with the heavy metal bioavailability in soil was noted from non-linear models. The transfer factors (TFs) were 4.49 for Cd and 1.39 for Zn. The Zn concentration in leaves under all treatments did not exceed threshold set standards, but Cd levels exceeded these standards when the concentration of Cd in the soil was more than 1.92 mg kg^?1 dry weight (dw). Data indicate that cole (Brassica) is not a suitable crop for oasis soils because of plant contamination with heavy metals, especially Cd.     

一株氯苯优势降解菌的降解条件优化

以氯苯降解率为降解效果指标,以降解温度、初始pH、降解时间、接种量和氯苯初始浓度为影响因素,对实验室保藏的一株氯苯优势降解菌株Lysinibacillus fusiformis LW13降解氯苯的降解条件进行优化。单因素试验结果表明,该降解菌株对氯苯的适宜降解条件分别为:温度20～40℃,pH为8.0,降解时间4 d,接种量2%～4%,氯苯初始浓度60～140 mg/L。以降解温度、氯苯初始浓度和接种量这三个显著影响因素进行正交试验,结果表明各影响因素的主次顺序为降解温度>氯苯初始浓度>接种量,最佳降解条件为降解温度35℃、氯苯初始浓度100 mg/L和接种量4%,最佳降解条件下氯苯降解率可高达93.8%。     

地形对日本福岛核事故放射性粒子扩散影响的敏感性研究

利用FLEXPART-WRF粒子扩散模式和Stohl等给出的福岛核事故137Cs排放数据,对日本福岛核事故放射性粒子扩散情况进行了数值模拟和有、无地形的敏感性试验;同时假定核污染物排放源位于日本高崎市的RN38站(36.3°N、139.1°E),对向东开口的V型特殊地形进行了敏感性试验. 福岛核事故数值模拟结果表明:放射性污染物的扩散路径、范围和强度既与天气形势有关,还与地形密切相关,不同天气或不同盛行风向条件下,地形的绕流和抬升作用对核污染物扩散输送态势的影响具有明显差异;在偏东气流影响下,由于地形作用,相比无地形时核污染物扩散偏西1个经度左右,偏高约0.5km. V型特殊地形敏感性试验结果表明:在合适的环流形势下(边界层为偏东风,中、高层为偏西风),由于V型地形的阻挡作用,可形成强烈上升运动区,核污染物主要向上、向东扩散输送,向上可扩散至3.0km以上,向东可扩散至145°E左右;而无地形时核污染物向上、向东影响范围减小,向东扩散至140°E,向上扩散至1.5km,但由于无地形阻挡和偏东风影响,向西扩散范围有所增加. 讨论了天气和地形条件对核电站选址的影响,不同天气形势下地形对核污染物扩散的影响并不相同,因此核电站选址应首先考虑选址地的大气环流背景和常见的天气系统,在此基础上再考虑地形的影响.      

正交实验选择纤维素酶产生菌的最优综合培养条件

在复合碳源、30℃恒温培养条件下,用正交实验法对影响纤维素酶产生菌降解纤维素的5种单因素培养条件进行了优化,并将优化得到的条件应用于厌氧-好氧废水处理系统.结果表明,MgSO4用量等单因素对纤维素酶活性以及纤维素降解率有不同程度的促进作用;正交实验得到厌氧菌和好氧菌的最佳培养条件不完全一致;在废水处理系统中,5种单因素的最佳综合水平为:30mg/L MgSO4,20mg/L CoCl2,CNP配比为400:5:1,氮源为NH4Cl (28.7mg/L), pH=7.0,此时厌氧菌及好氧菌酶活性分别为4801U/L和4794U/L,酶稳定性分别达到91.0%和95.5%,纤维素降解率为31.9%和28.4% .     

改性斜发沸石处理高浓度氨氮废水

采用NaOH碱熔法对缙云斜发沸石进行处理,采用正交实验对碱熔法改性沸石的最佳条件进行了选择;并对改性前后的沸石进行粉末X射线衍射(XRD)、电感耦合等离子体发射光谱法(ICP-AES)和扫描电镜(SEM)表征;详细研究了所得改性沸石在氨氮废水处理中的净化性能.结果表明,处理沸石的水热温度对氨氮去除效果的影响最显著;碱熔法处理可使缙云斜发沸石转变为低硅铝比的Na-P型分子筛,它对氨氮废水的NH4+-N具有优异的吸附性能.当改性沸石投加量为5 g,对100 mL浓度为1000 mg.L-1氨氮溶液,氨氮去除率可达77.8%,改性沸石吸附NH4+-N是一快速吸附过程,且能较好地符合Langmuir吸附等温模式,偏向于单分子层的吸附.     

Are experienced people affected by a pre-set default option—Results from a field experiment

The objective of the present paper is to investigate the robustness of the well-known result that pre-set default options determine people's choices. We do so by conducting a field experiment among environmental economists attending a large international conference on environmental economics. The participants were, at the time of registration, randomly allocated to different treatments related to carbon offsetting. What differs from earlier default studies is that our subjects have good knowledge about the good at hand. We investigate whether the choices of these experienced individuals are affected by a pre-set default option, and we also study the effect of a treatment with no pre-set default option. Our results, together with previous findings, indicate that the effect of a default option attenuates with experience.     

选择试验模型法在耕地资源保护中的应用——以浙江省温岭市为例

选择试验模型法是目前国际上用于评估具有公共物品特性的自然资源和环境物品经济价值的一种相对较新的方法。论文以浙江省温岭市耕地资源保护为例,探讨选择试验模型法在耕地资源保护经济价值评估实践的可行性。在专家咨询和小组讨论的基础上,确定耕地景观、田间设施、土壤肥力和耕地保护费为温岭市耕地资源保护的4个属性。在对温岭市246名居民进行随机抽样调查的基础上,分析了温岭市耕地资源保护不同属性的价值以及耕地资源保护不同替代方案的相对价值。结果表明,对于温岭市全体居民来说,今后耕地资源保护的实施应该重点改善田间设施和提高土壤肥力,而耕地景观的改善也同样可以增加温岭市居民的福利水平。