28-Homobrassinolide mitigates boron induced toxicity through enhanced antioxidant system in Vigna radiata plants

The objective of this study was to establish relationship between boron induced oxidative stress and antioxidant system in Vigna radiata plants and also to investigate whether brassinosteroids will enhance the level of antioxidant system that could confer tolerance to the plants from the boron induced oxidative stress. The mung bean (V. radiata cv. T-44) plants were administered with 0.50, 1.0 and 2.0 mM boron at 6 d stage for 7 d along with nutrient solution. At 13 d stage, the seedlings were sprayed with deionized water (control) or 10⁻⁸ M of 28-homobrassinolide and plants were harvested at 21 d stage to assess growth, leaf gas-exchange traits and biochemical parameters. The boron treatments diminished growth, water relations and photosynthetic attributes along with nitrate reductase and carbonic anhydrase activity in the concentration dependent manner whereas, it enhanced lipid peroxidation, electrolyte leakage, accumulation of H₂O₂ as well as proline, and various antioxidant enzymes in the leaves of mung bean which were more pronounced at higher concentrations of boron. However, the follow-up application of 28-homobrassinolide to the boron stressed plants improved growth, water relations and photosynthesis and further enhanced the various antioxidant enzymes viz. catalase, peroxidase and superoxide dismutase and content of proline. The elevated level of antioxidant enzymes as well as proline could have conferred tolerance to the B-stressed plants resulting in improved growth, water relations and photosynthetic attributes.     

Histopathological and biochemical alternations of the heart induced by acute cadmium exposure in the freshwater crab Sinopotamon yangtsekiense

Cadmium (Cd) is a highly toxic element in water. Its toxicity has been attributed to oxidative stress mediated by free radicals. Here we investigated the effects of Cd on the histopathology, antioxidant enzymes and lipid peroxidation of crustacean heart. The freshwater crabs Sinopotamon yangtsekiense were exposed to different concentrations of Cd for 1, 3, 5 and 7 d. After exposure, histological abnormalities were discovered, including myocardial edema, vacuolar and vitreous degeneration, and infiltration of inflammatory cells. Additionally, alterations in nuclei, mitochondria, rough endoplasmic reticulum as well as myofibrils were observed. Meanwhile, superoxide dismutase (SOD) activity was significantly increased after Cd exposure. Catalase (CAT) activity was only increased in the group exposed to 14.50 mg L⁻¹ Cd on day 5 and decreased with increasing Cd concentration and exposure time. Glutathione peroxidase (GPx) activity was increased in groups treated with 29.00, 58.00 and 116.00 mg L⁻¹ on days 1 and 3, and decreased thereafter. Besides, malondialdehyde (MDA) levels were significantly increased after 3 d of Cd exposure at all the indicated concentrations. These results showed that acute Cd exposure led to harmful effects on the histology of crab heart, which are most likely linked to Cd-induced oxidative stress.     

Impact of dissolved organic matter on bioavailability of chlorotoluron to wheat

Chlorotoluron (Chl) is a phenylurea herbicide and is widely used for controlling weeds. While it has brought great benefits to crop production, it has also resulted in contamination to ecosystem. In this study, we investigated accumulation of chlorotoluron (Chl) and biological responses of wheat plants as affected by dissolved organic matter (DOM). Wheat seedlings grown under 10 mg kg⁻¹ Chl for 4 d showed a low level of chlorophyll accumulation and damage to plasma membrane. The growth was inhibited by exposure of chlorotoluron. Treatment with 50 mg DOC kg⁻¹ DOM derived either from sludge (DOM-SL) or straw (DOM-ST) attenuated the chlorotoluron toxicity to plants. Both DOMs decreased activities of catalase, peroxidase and superoxide dismutase in Chl-treated seedlings. However, an increased glutathione S-transferases activity was observed under the same condition. Wheat plants treated with Chl in the presence of DOM accumulated less Chl than those treated with Chl alone. Moreover, in the presence of DOM, bioconcentration factor (BCF) decreased whereas translocation factors increased. Analyses with FT-IR spectra confirmed the regulatory role of DOMs in reducing Chl accumulation in wheat.     

Oxidative stress in duckweed (Lemna minor L.) caused by short-term cadmium exposure

The mechanisms of plant defence against cadmium toxicity have been studied by short-term exposure of Lemna minor L. (common duckweed) to concentrations of CdCl2 ranging from 0 to 500microM. High accumulation of cadmium was observed (12,320+/-2155microgg(-1) at 500microM CdCl2), which caused a gradual decrease of plant growth, increased lipid peroxidation, and weakened the entire antioxidative defence. Total glutathione concentration decreased significantly; however, the concentration of oxidized glutathione remained stable. The responses of four antioxidant enzymes showed that catalase was the most inhibited after CdCl2 exposure, ascorbate peroxidase and guaiacol peroxidase moderately, and glutathione reductase least. The total antioxidative potential revealed an induced antioxidative network at 0.1microM CdCl2 (137+/-13.2% of the control) and its reduction to only 47.4+/-4.0% of the control at higher cadmium concentrations. The possible application of the examined biomarkers in ecotoxicological research is discussed.     

渤海油田中深层低含H2S气井油套管选材研究

目的 解决渤海油田中深层低含H2S井下环境的油套管安全选材问题.方法 利用高压釜模拟气井的腐蚀环境,对不同材质的备选油套管钢在不同井深模拟工况条件下的腐蚀速率和应力腐蚀开裂行为进行研究,采用电子扫描显微镜(SEM)及其能谱仪(EDS)和X射线衍射分析仪(XRD),分析或检测腐蚀形貌、腐蚀产物的组成,综合考虑均匀腐蚀和开...     

复杂应力条件下地基岩石细观破坏研究

为了研究复杂应力条件下的深基坑岩石细观破坏问题,本构方程采用由应变空间导出的弹塑性损伤细观力学模型,借助有限元计算方法,实现了岩石三维破裂过程的数值模拟。采用细观破坏单元网格消去法,实现了有限元模拟裂纹扩展过程;利用位移加载来实现岩石逐渐破裂过程;数值模拟灰岩单轴拉伸及压缩破坏试验、双轴拉伸破坏试验和三轴受压破坏试验,得到其非线性应力—应变曲线和不同载荷阶段弹塑性损伤破裂演化系列图像;分析细观非均匀性对岩石宏观破裂力学行为的影响。研究表明,在复杂应力条件下,随着围压增大,峰值抗压强度明显提高,塑性变形明显增大。本文研究对深基坑施工过程中预防工程事故的发生具有借鉴意义。     

专业认证背景下的安全工程专业特色教育研究与实践

回顾专业认证在国内外的发展情况,简单介绍《安全工程专业本科教育认证标准》的内容和安全工程专业认证近年来在国内的发展情况。根据南华大学的实际情况,提出在专业认证的背景下,针对行业特点,实行分方向设置课程和从事教学活动。南华大学安全工程专业主要依托"核"行业背景,下设核安全、建筑安全和化工安全3个方向;3个专业方向分别设置了不同的基础课、专业基础课、专业课和选修课;课程设置符合《安全工程专业本科教育认证标准》的要求,并严格按照教学计划开展教学。实践证明,该项教学改革取得明显成效,该校安全工程专业近两年的就业率和考研率都居于全校前列,并且就业单位具有明显的行业特点。     

西南生态脆弱区类型及其特征分析

对生态脆弱区涵义加以界定,并全面阐述和分析了生态脆弱区具有特征的和性质。根据西南地区各区域生态与环境脆弱特征，结合自然环境特征和人类活动特点，以胁迫源、胁迫因子的交互作用和胁迫过程为主导。确定西南生态脆弱区的类型有5种，即：中度胁迫中度生态脆弱区、重度胁迫高度生态脆弱区、低度胁迫高度生态脆弱区、重度胁迫中度生态脆弱区、中度胁迫高度生态脆弱区。     

油气输送焊管残余应力测试与安全评价方法研究

针对油气输送焊管残余应力测试与评估技术难题,采用机械切割应力释放法对不同成型工艺和状态焊管的残余应力进行了测试分析对比,结果表明:UOE焊管的残余应力分布水平较低;ERW焊管次之;SSAW焊管的残余应力分布范围较广,有高有低。不同管型和成型工艺及水压试验状态具有明显不同的残余应力分布特征,在进行焊管安全评价时必须区别对待。根据残余应力实测结果提出了焊管安全评价时残余应力的取值建议。研究结果对管道设计、安全分析与评价、焊管残余应力的改善、控制及焊管合理选用具有重要工程意义。