Quantification of metallothionein in whole body gammarus locusta (crustacea: amphipoda) using differential pulse polarography

A polarographic method was optimised for the quantitative determination of metallothionein (MT) in whole body Gammarus locusta (Crustacea: Amphipoda). The identity of the MT-like proteins was examined using electrophoretic analysis (SDS-PAGE) and induction studies (water-borne exposures to copper). The MT-method was then used to examine changes in MT levels over the lifetime (juveniles, sub-adults and adults) of a laboratory population of G. locusta; copper (Cu) and zinc (Zn) body burden were also measured by flame atomic absorption spectrometry. MT was quantified by heat-denaturation of 30?000?g supernatants (to remove the bulk of non-MT proteins) followed by differential pulse polarography (DPP) to measure sulphydryl-group containing proteins. DPP proved to be a sensitive and reliable method to quantify heat-stable total sulphydryl-proteins. The majority of the latter was indicated to be MT from SDS-PAGE and induction studies. SDS-PAGE revealed a putative MT of 23?±?0.6?kDa app. mol. wt., consistent with the average size of dimeric MTs (10–20?kDa) found in several aquatic invertebrate species. Copper-exposure revealed parallel increases in the amounts of 23?kDa protein and total sulphydryl-proteins. Levels of whole body MT (rabbit pure MT as standard) range between 1.3 and 2.3?mg?g^?1 dry wt. over the lifetime of G. locusta. Sex-related changes in MT status were observed, the levels in females being 1.6 times higher than in males (p<0.05). Copper and Zn body content changed over the lifetime of G. locusta, the levels in juveniles (p>0.05) and sub-adults (p<0.05) being higher than in adults. It is concluded that the inclusion of MT in ecotoxicological studies with amphipod crustaceans require some caution with respect to the stages of moulting and/or sexual maturation of organisms, but this should not provide any major impediment in its use in metal pollution monitoring.     

不同类型除尘器改造为脉冲除尘器技术

介绍了除尘器改造的原则、技术和方法,并结合多个除尘器改造的工程实例,分享了不同类型除尘器改造为脉冲除尘器成功经验。经过改造的除尘器能够满足集气吸尘罩处风量的要求,减少了除尘器出口排放浓度,降低了除尘器的运行阻力。     

新型复合式除尘器

针对电、布除尘器的特点,提出了一种新型静电布袋混合式除尘器,并已应用于工业中。在没有使用任意阀门的前提下,该产品将电除尘器和布袋除尘器结合在一起,取长补短,充分发挥了电除尘器能大量收尘、节能、维护检修工作量小,还可捕集高比电阻和低比电阻等特殊干性粉尘,解决了电除尘难以净化的含微米或亚微米烟尘的难题。兼具布袋除尘器对粉尘粒度和比电阻不敏感、排放浓度低的优点。一级静电除尘器效率可达90%,对于粉尘浓度为30 g/m3左右的燃煤锅炉,经过静电除尘后进入滤袋的粉尘浓度只有3 g/m3。对于这种粉尘浓度,滤袋的清灰周期可达3 h以上。由于清灰周期的大大增长,可大大提高滤袋的寿命。该产品性价比优良、占地面积少、结构简单。尤其在净化效率、气体阻力等主要技术指标方面达到了环保要求。     

中铝洛铜熔铸厂16吨黄铜炉组除尘系统改造

通过对中铝洛铜熔铸厂16t黄铜炉组除尘系统出现的问题采取相应对策进行改造,探讨了通过屋顶罩排烟系统来解决黄铜炉组炉盖开启操作时产生大量烟尘的方法,具有实用性,同时针对黄铜炉熔炼产生烟尘的特点,提出了适合氧化锌回收的除尘改造方案.     

FND- CASS工艺处理城市污水的试验研究

为进一步提高循环式活性污泥工艺(CASS)的脱氮除磷效果,改变其运行方式,采用好氧脉冲曝气,提出频繁硝化-反硝化循环式活性污泥工艺(FND-CASS).结果表明,FND-CASS工艺与CASS工艺相比,在去除污水中有机物方面能力略有下降,但在脱氮除磷方面具有明显的优势.在同样的进水流量且DO浓度控制较低的运行条件下,F...     

高速铁路声屏障

提出我国高速铁路(铁路客运专线)声屏障主要结构形式和应有的性能。重点分析高速列车气动力的形成、影响因素、计算方法及其对声屏障的作用。提出了高速铁路声屏障的设计要点及其对声屏障的设计建造的建议。     

低压脉冲袋式收尘技术在银转炉有价烟尘回收系统中的应用

通过对精心设计后的某有色行业金银溶炼收尘系统的介绍,对比分析了新老两代脉冲袋式收尘技术的性能参数。阐明了在回收该系统有价金属粉尘中,以低压快速脉冲袋式收尘器替代传统高压脉冲袋式收尘器后的成功应用,以及取得的较好社会效益、经济效益和环境效益。     

长袋低压脉冲袋式除尘器在水泥立窑烟气除尘上的应用

比较了几种用于水泥立窑烟气除尘装置后 ,长袋低压脉冲袋式除尘器的优势凸现 ,对其在广州新华水泥厂实际使用出现的问题作了详细分析 ,并提出了解决办法     

浅谈河道与水库清淤的新工艺

分析比较了传统河道水库淤积的清除工艺 ,从而提出一种新型的清淤技术 ,即气举与自激振荡脉冲水射流联合作用下的清淤工艺。分析了这种新工艺的清淤原理 ,论证了其对水下坚硬沉积物的破碎与抽取的连续作用机理 ;研究了空压机的工作压力与清淤量的关系。     

高压脉冲等离子体催化还原NO的研究

用离子交换法制备Co-ZSM-5催化剂,考察了有氧条件下,在甲烷选择还原NO的反应中,高压脉冲等离子体的加入对Co-ZSM-5活性的影响。发现在一定温度范围内,高压脉冲等离子体与Co-ZSM-5之间存在协同效应。在此基础上,初步探讨了高压脉冲等离子体活化CH4催化还原净化NO的反应机理。