Exploration of the key functional proteins from an efficient cellulolytic microbial consortium using dilution-to-extinction approach

In the present study, the cellulose binding proteins(CBPs) secreted by a putative cellulolytic microbial consortium were isolated and purified by affinity digestion. The purified CBPs were subsequently separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis(SDS-PAGE). Using mass spectrometric analyses, eight CBPs were identified and annotated to be similar to known proteins secreted by Clostridium clariflavum DSM 19732 and Paenibacillus sp. W-61. In addition, in combination with dilution-to-extinction approach and zymogram analysis technique, CBPs 6(97 k Da) and 12(52 k Da) were confirmed to be the key functional proteins that influence cellulolytic activities. Moreover, structural domain analyses and enzymatic activity detection indicated that CBPs 6 and 12 contained glycoside hydrolase families(GH) 9 and 48 catalytic modules, which both revealed endoglucandase and xylanase activities. It was suggested that the coexistence of GH9 and GH48 catalytic domains present in these two proteins could synergistically promote the efficient degradation of cellulose.     

平朔露天煤矿排土场植被恢复树木短期死亡动态研究

以平朔露天煤矿排土场0.8 hm2刺槐+油松混交林复垦模式下固定监测样地为研究平台,以2010年和2015年两次调查数据为基础,分析了五年间样地内主要树种的短期死亡动态.结果表明:样地内所有树木总个体数由1026株减少到947株,死亡个体79株,占2010年总个体数的7.7%,刺槐和油松的平均胸径增量分别为1.44 cm和1.92 cm,样地内所有树木的胸高断面积增加2.3 m2·hm-2,中径级个体(7 cm相似文献   

如何完善沥青路面结构设计保证车辆行驶安全的探讨

沥青路面的病害直接威胁着公路通行车辆行驶安全,沥青路面的结构设计不合理是导致路面发生病害的重要原因之一。通过对高速公路沥青路面的主要病害成因从设计角度进行分析,提出应从完善路面设计基础数据的测试与确定方法、合理选择原材料、采用合适的矿料级配、完善配合比设计方法、合理选择沥青面层厚度、加强沥青面层层间及与基层的粘结等途径完善沥青路面结构设计,以进一步提高沥青路面的使用功能,减少道路交通安全事故的发生。     

价格对中国煤矿事故的影响分析

针对我国目前煤炭产业严峻的安全形势，本文采用、统计的方法，分析了煤炭价格和产量与煤矿安全生产事故之间的相关关系，验证煤炭价格与煤矿安全生产事故的发生存在协整关系，而且煤价是煤矿伤亡事故的致因，并进一步做回归分析，并尝试从价格的角度揭示煤矿安全生产事故发生的规律。     

森林旅游资源开发潜力定量评价研究--以武汉市为例

森林旅游资源定量评价是为发展森林旅游提供更为可靠的依据。研究了森林旅游资源开发潜力的定量方法，对武汉市森林旅游资源开发潜力进行了定量评价，得出了武汉市森林旅游资源的质量等级，为森林旅游的开发提供了可靠的依据。     

中国实行洪水保险的可行性研究

在回顾洪水保险实施现状的基础上，指出了我国洪水保险与世界洪水保险的差距，根据我国洪水灾害的特点和我国经济体制的特点，提出了符合我国国情的洪水保险措施：建立洪水保险准备金是进行洪水保险的前提，洪水保险的特殊性决定了洪水保险不可能是个营利性的保险，因此要保证洪水保险的正常运行必须有社会和国家的扶持，洪水保险机构应按流域设立，中国洪水灾害发生的空间特点决定了洪水保险机构只能按流域设置，洪水灾害发生的时间特点决定了洪水保险的对象必须是固定资产，而不应该是流动资产，遭受洪水灾害影响最大的是农田，因此农田应该是洪水保险的对象，不应该是种植在农田里的农作物，另外可以通过农业保险对不同农作物进行保险，洪水保险需要洪水风险的分析和研究作技术支撑，没有科学的洪水风险分析，洪水保险很难顺利进行，加强与洪水保险有关的各方面法律法规的建立，在洪水保险法中明确界定洪水保险的保险对象，明确保险双方的义务和权利。     

DISCUSSION ON REGION RESOURCE ADVANTAGE OF THE WEST CHINA AND APPROACHES OF ITS INDUSTRIALIZATION DEVELOPMENT

With 70 .82 %ofthetotallandarea ,2 7.6 7%ofthetotalpopulationandrelativelyabundantnaturalresources,thevastwestChinaisextremelyimportantinstrategies.HowtomanagetheeconomicdevelopmentinthewestChinarapidlyhasarousedgreatattentionsinallwalksoflife .Theimportantpoliciesandmethodsaremainlydependingonthelarge scaleex plorationofnaturalresourcesanddevelopmentofrelevantindustries.However ,themisunder standingoflarge scalemineralexplorationsinthewestwillleadtoanoldroadofviciouscircula tion .Becauseofth…     

含氮杂环化合物环境参数及生物活性的QSAR研究

定义了原子的价点价值δi，由δ1^Y构建了新的分子结构信息价连接性拓扑指数mX^Y(m=0,1,2,…，n)，并计算了13种含氮杂环化合物的mX^Y(m=0，1，2，3)值，发现mX…Y与含氮杂环化合物的水溶解度、正辛醇／水分配系数和对发光菌、大型蚤的毒性有良好的相关性。与其他的指数相比较，新指数结构选择性和相关性好，且新方法还具有计算值简单，物理意义明确等优点。并对-lgEC50=α2 b2^0X^Y b3^1X^Y模型采用Jackknife法予以检验，具有总体稳健性。     

Realising local food policies: a comparison between Toronto and the Brussels-Capital Region’s stories through the lenses of reflexivity and co-learning

ABSTRACT

This paper revisits the development of Toronto and Brussels’ local food policies by analysing reflexivity and co-learning as important dimensions within a Hybrid Governance Approach (HGA); it approaches the interaction between four forms of governance (bottom-up, networked, state, market-led) and the tensions between them as hybrid dynamics. Within this approach, reflexivity refers to the positionality of agents, i.e. to the ways local food actors embody as well as reflect on and reconsider their principles and practices through time. Closely related to reflexivity, co-learning involves agential interactions to co-construct enabling food policy delivery systems. The HGA is mobilised to understand the ways in which reflexive capacities, as well as co-learning, take place in the two cases and how they lay the basis of particular modes of (de)institutionalisation. Learning from the two cases’ trajectories, this paper highlights: (a) the role of key governance tensions as triggers as well as breeding grounds for reflexivity and co-learning outcomes; (b) the challenges of food movement actors to surmount or valorise key tensions in order to build accountable modes of food policy delivery through time; (c) the struggles to build legitimacy and accountability in local food movements through the development of bottom-linked organisations and governance modes.     

产毒微囊藻mcyA基因荧光定量PCR方法的建立

针对微囊藻毒素合成酶基因mcyA探索一种适用于自然水样中微囊藻毒素产毒潜能检测的TaqMan实时荧光定量PCR方法.该方法从铜绿微囊藻FACHB-1279中的mcyA基因中扩增出目的基因片段,用TA克隆方法将基因片段插入Allel TA vector (ABP-CETAVEC020)中来制备质粒标准晶.通过不同连续稀释...