枯草芽孢杆菌对Cu2+的吸附及菌体表面基团分析

以枯草芽孢杆菌(Bacillus subtilis)为生物吸附剂,讨论了其对Cu2+的生物吸附规律,并通过酸碱滴定这一表面分析手段,结合相关软件及傅里叶变换红外光谱(FTIR)分析了菌体表面主要的基团种类及数量.结果表明,枯草芽孢杆菌对Cu2+吸附的最佳条件是:pH为6、吸附时间为24 h,菌悬液用量和Cu2+初始浓度...     

The policy consequences of defining rewilding

More than 30 years after it was first proposed as a biodiversity conservation strategy, rewilding remains a controversial concept. There is currently little agreement about what the goals of rewilding are, and how these are best achieved, limiting the utility of rewilding in mainstream conservation. Achieving consensus about rewilding requires agreeing about what “wild” means, but many different definitions exist, reflecting the diversity of values in conservation. There are three key debates that must be addressed to find a consensual definition of “wild”: (1) to which extent can people and “wild” nature co-exist?; (2) how much space does “wild” nature need? and (3) what kinds of “wild” nature do we value? Depending on the kinds of “wild” nature rewilding aims to create, rewilding policy will be faced with managing different opportunities and risks for biodiversity and people.Supplementary InformationThe online version contains supplementary material available at 10.1007/s13280-021-01560-8.     

采油厂联合站生产作业条件危险性评价

生产作业条件危险性评价法是对生产作业单元进行的危险性评价,其评价结果可以比较直观地反映出生产作业各岗位的危险程度,为企业安全管理提供指导.在对采油厂联合站各岗位深入调查的基础上,分析和评价了该站11个岗位的作业条件危险性,进行了危险度分级,确定了重大危险岗位.     

皖江城市带温泉旅游地定量评价及开发策略

运用专家咨询法,从温泉资源质量、市场区位条件、区域支持条件3个方面构建了涵盖13个评价因子的温泉型旅游地开发评价指标体系。为最大限度降低评价的主观性,将评价因子分为数值型和模糊型两种,综合运用菲什拜因 罗森伯格模型和模糊数学模型,在深入调研皖江城市带各温泉旅游地并掌握相关数据的基础上,从市域层面对皖江城市带6个地区的温泉旅游开发条件进行了量化评价。结果显示: 合肥、马鞍山开发条件最优,其温泉旅游资源与市场区位及区域支持条件匹配良好;安庆、六安次之,宣城、池州较差。最后结合评价结论,先从文化和竞合2个方面提出皖江城市带温泉旅游地整体开发和提升的策略,再针对得分不同地区的开发现状和所处阶段,分别提出“共生模式”和“提升模式”两大对策     

大连市旅游业发展与经济增长关系研究

采用Johansen协整检验、误差修正机制、Granger因果检验、脉冲响应分析和方差分解等计量方法,对大连市旅游业发展和经济增长之间的关系进行了研究。结果表明,大连市旅游业发展和经济增长存在长期均衡关系,存在从旅游业到经济增长的单向因果关系。在此基础上,提出进一步发展大连市旅游业的对策建议。     

Surface-enhanced Raman spectroscopic analysis of N6-benzylaminopurine residue quantity in sprouts with gold nanoparticles

ABSTRACT

A rapid and quantitative method for the determination of N⁶-Benzylademine (N⁶-BA) was established through the application of surface-enhanced Raman spectroscopy (SERS). The Raman peak intensities of N⁶-BA at 1002 cm^?1 positively correlated to N⁶-BA concentrations in sprout extracts. The R² reached 0.99, and RSDs calculated below 10% at the concentration range of 0.1 ～5μg mL^?1. The average recoveries were 80.0% ～ 98.2% for blank samples intentionally contaminated at differing levels of 0.04, 0.4, and 1 μg g^?1. The whole procedure, including sample preparation and SERS detection, did not exceed 30 min for a set of 6 samples. This study indicates that SERS is a promising technique for rapid tracing analysis and on-site testing of N⁶-BA.     

人类特异性粪厌氧菌基因标记物实时荧光定量PCR检测方法的建立和应用

针对人类粪便污染,选择来源于拟杆菌和双歧杆菌16S rRNA的基因片段作为标记物,分别建立了相应的实时荧光定量PCR检测方法.选取不同来源的粪便样品进行检测,证实了引物的特异性.回收纯化从污水中扩增所得的目标PCR片段,经过连接转化,筛选阳性克隆提取其重组质粒作为实时荧光定量PCR的标准品.通过检测一系列梯度稀释的标准品,确定了拟杆菌基因标记物和双歧杆菌基因标记物定量PCR检测方法分别在1.98×102～1.98×107 copy/μL和2.12×101～2.12×107 copy/μL范围内具有良好的线性关系.     

Long-range transport of acidifying substances in East Asia—Part II: Source–receptor relationships

Region-to-grid source–receptor (S/R) relationships are established for sulfur and reactive nitrogen deposition in East Asia, using the Eulerian-type Community Multiscale Air Quality (CMAQ) model with emission and meteorology data for 2001. We proposed a source region attribution methodology by analyzing the non-linear responses of the CMAQ model to emission changes. Sensitivity simulations were conducted where emissions of SO₂, NO_x, and primary particles from a source region were reduced by 25%. The difference between the base and sensitivity simulations was multiplied by a factor of four, and then defined as the contribution from that source region. The transboundary influence exhibits strong seasonal variation and generally peaks during the dry seasons. Long-range transport from eastern China contributes a significant percentage (>20%) of anthropogenic reactive nitrogen as well as sulfur deposition in East Asia. At the same time, northwestern China receives approximately 35% of its sulfur load and 45% of its nitrogen load from foreign emissions. Sulfur emissions from Miyakejima and other volcanoes contribute approximately 50% of the sulfur load in Japan in 2001. Sulfur inflows from regions outside the study domain, which is attributed by using boundary conditions derived from the MOZART global atmospheric chemistry model, are pronounced (10–40%) over most parts of Asia. Compared with previous studies using simple Lagrangian models, our results indicate higher influence from long-range transport. The estimated S/R relationships are believed to be more realistic since they include global influence as well as internal interactions among different parts of China.     

区域社会经济发展与生态环境耦合关系研究模型的比较分析

社会经济发展与环境资源相协调是可持续发展的重要途径,判断当前区域社会经济发展与生态环境间的耦合关系是建立有效协调机制的关键。本文从发展、机理方法和应用实例等方面系统介绍了目前在社会经济发展与生态环境之间耦合关系研究中主要的3类模型:计量经济学模型(环境库兹涅茨曲线)、耦合度定量判断模型(包括灰色关联度分析和数理模型)和系统动力学模型。通过3类模型机理和应用情况的对照分析,总结各自优劣势,并认为具有多情景分析和多方案执行评估能力的神经网络模型将成为未来发展趋势。