采用尼罗红荧光探针对微藻中油脂的定量测定

以尼罗红为荧光探针,对小球藻油脂含量的定量分析条件进行了研究.测定中,使用48孔酶标仪作为荧光检测器.研究显示,当荧光激发光波长为485 nm,发射光波长为580 nm时,适用于测定微藻中的中性油脂含量.当二甲基亚砜终浓度为20%,尼罗红终浓度为0.5μg.mL-1,藻液与尼罗红置于40℃暗处反应5 min后,能够获得最大的相对荧光测定值.当藻液光密度OD680在0.61至1.73时,藻液油脂含量与尼罗红荧光测定值呈现良好的线性关系,相关系数R2为0.9836,可以通过尼罗红荧光值的测定推算油脂的含量.     

Genotoxic and cytotoxic in vitro evaluation of ivermectin and its formulation ivomec® on Aedes albopictus larvae (CCL-126™) cells

Genotoxic effects of ivermectin (IVM) and its commercial formulation ivomec® (IVM 1.0%) were studied on Aedes albopictus larvae (CCL-126?) cells by sister chromatid exchange (SCE) and single cell gel electrophoresis (SCGE) while cytotoxicity was determined by cell-cycle progression (CCP), proliferative rate index (PRI), mitotic index (MI), 3(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and neutral red (NR) endpoints within a 1–250 µg mL^?1 concentration range. While IVM and ivomec® did not markedly affect SCE frequencies, these agents induced DNA-strand breaks enhancing both slightly damaged and damaged cells at 25–50 and 5–50 µg mL^?1 IVM and ivomec®, respectively. Both compounds exerted a delay in CCP and reduction of PRI at 10 µg mL^?1. Cytotoxicity was observed at concentrations higher than 25 µg mL^?1. A marked reduction of about 98% and 94% of MI compared to controls was noted with 25 µg mL^?1 of IVM and ivomec®, respectively. NR and MTT assays revealed that both compounds induced a cell growth inhibition within the 1–250 µg mL^?1 concentration range. Data indicated that IVM and ivomec® exert both genotoxicity and cytotoxicity in insect cells in vitro, at least in A. albopictus larvae CCL-126? cells.     

一株花生根际促生菌的筛选鉴定及其特性研究

从种植于红壤的健康花生根际,筛选出7株产吲哚乙酸(IAA)菌株,以菌株L4合成IAA的能力最强,培养24 h时IAA产生量达135.67μg.mL-1,且菌株L4具有解磷能力。通过菌株形态、生理生化特征测定及16SrRNA的保守序列鉴定,初步确定菌株L4为氯酚节杆菌(Arthrobacter chlorophenolicus),其GenBank登录号为JQ277449。菌株生长和发酵条件试验结果表明,菌株L4生长和分泌IAA的最佳培养条件并不完全一致,既能促进菌株生长又能合成较多IAA的最佳培养条件是初始pH值为5～6,装液量为50 mL.(250 mL)-1,30℃摇床培养24 h;促进菌株生长的最佳碳、氮源分别是麦芽糖和酵母粉,而提高IAA产生量的最佳碳源是木糖,最佳氮源是KNO3。     

铁路车务系统安全保障体系研究

以分析铁路车务系统安全管理现状为出发点,提出了"红线"管理理论,运用系统安全理论,构建了铁路车务系统安全保障体系框架,具体包括安全预防体系和应急响应体系两部分,并且分别从理论、技术和"红线"管理理论三方面进行了分析。     

苍头赤泥灰渣堆场安全性分析

介绍了苍头赤泥灰渣堆场情况,从坝体位移观测、环境安全、防洪方面对该堆场进行了分析。认为苍头赤泥灰渣堆场处于安全状态,并对坝体管理提出建议。该分析方法对同类尾矿库的安全性评价具有指导意义。     

MgO改性赤泥复合材料对废水中氮磷的同步回收

为处理高浓度氮磷废水同时实现赤泥资源化利用,通过赤泥负载氧化镁制备高效氮磷回收材料(MgO-RM),用以对废水进行氮磷同步回收.考察了废水初始pH值、废水氮磷比和MgO-RM投加量对氮磷同步回收效果的影响.采用动力学模型和等温吸附模型对回收特性进行了描述,在此基础上利用FTIR、XRD、SEM、BET测试手段对MgO-...     

生态保护红线分区建设模式研究——以广东大鹏半岛为例

生态保护红线是中国一项创新性的环境保护制度,但目前其划分和实施管理机制仍处于不断探索阶段。以大鹏半岛为研究区域开展生态保护红线分区模式研究。通过生态调查和GIS空间分析相结合,综合分析大鹏半岛的功能定位、地形地貌特征、典型生态系统类型、珍稀濒危动植物资源和自然遗迹的分布状况、生态系统完整性和连续性特征,构建了全面反映生态特征的生态保护红线分区建设模式,提出基本生态控制线内差别化管理的思路,探讨了分区、分类、分级的生态保护管理体系,创新了流域生态管理模式。以基本生态控制线为基础划分7个重点区域进行分类分级保护模式,重点保护面积为171.59 km2,占基本生态控制线面积的77.3%。重点保护区域包括大鹏半岛国家地质公园、大鹏半岛自然保护区、红花岭海岸森林公园、大鹏半岛中部生态廊道、坝光古银叶树Heritiera littoralis国家湿地公园、东涌红树林湿地公园、西涌香蒲桃Syzygium odoratum林自然保护小区。该模式的实施既能较好地保护大鹏半岛具有重要生态价值的资源,也能够协调经济社会发展和生态保护的关系,是深圳市基本生态控制线内分区管理制度的有益尝试,为生态保护红线的可持续性管理提供技术支撑,也为中国城市生态保护红线的划分体系和精细化管理提供参考。     

纳米TiO2催化声化学降解酸性红B的研究

以经过高温活化处理的锐钛型纳米TiO2为催化剂,考察了各种因素对酸性红B溶液超声降解反应的影响。结果表明:锐钛型纳米TiO2对酸性红B溶液超声降解反应具有明显的催化作用,其降解效果明显好于单纯使用超声波降解。超声频率40 kHz,输出功率50 W,催化剂用量0.5 g/L,pH=3.0,酸性红B溶液的初始浓度20 mg/L的条件下,60 min降解率即可达80%左右,120 min基本降解完全。因此,纳米锐钛型TiO2催化超声降解偶氮染料的方法切实可行,而且具有良好的应用前景。     

Conducting conservation social science surveys online

The coronavirus (COVID-19) pandemic is affecting the environment and conservation research in fundamental ways. Many conservation social scientists are now administering survey questionnaires online, but they must do so while ensuring rigor in data collection. Further, they must address a suite of unique challenges, such as the increasing use of mobile devices by participants and avoiding bots or other survey fraud. We reviewed recent literature on online survey methods to examine the state of the field related to online data collection and dissemination. We illustrate the review with examples of key methodological decisions made during a recent national study of people who feed wild birds, in which survey respondents were recruited through an online panel and a sample generated via a project participant list. Conducting surveys online affords new opportunities for participant recruitment, design, and pilot testing. For instance, online survey panels can provide quick access to large and diverse samples of people. Based on the literature review and our own experiences, we suggest that to ensure high-quality online surveys one should account for potential sampling and nonresponse error, design survey instruments for use on multiple devices, test the instrument, and use multiple protocols to identify data quality problems. We also suggest that research funders, journal editors, and policy makers can all play a role in ensuring high-quality survey data are used to inform effective conservation programs and policies.