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111.
Cielo Emar M. Paraoan Windell L. Rivera 《Journal of environmental science and health. Part. B》2017,52(5):306-313
Contaminated irrigation water may greatly affect not only the quality of produce but also the people exposed to it. In this study, agricultural irrigation waters in Bulacan, Philippines were assessed and found to be contaminated with Escherichia coli (E. coli) ranging from 0.58 to 4.51 log10 CFU/mL. A total of 79 isolates of E. coli were confirmed through polymerase chain reaction (PCR) amplifying the uidA gene and were tested for phenotypic resistance using 10 antimicrobials through the Kirby–Bauer disc diffusion method. Forty-six isolates (58.22%) were noted to be multidrug resistant (MDR) with high resistance rate to cephalothin, tetracycline, streptomycin, ampicillin, trimethoprim, nalidixic acid, and chloramphenicol. Moreover, this study also examined the prevalence of Class I and II integrons accounting to 67.39% and 17.39%, respectively, of the MDR E. coli strains using multiplex PCR. The results imply that the agricultural water used in Bulacan is contaminated with the fecal material of man or other animals present in the area, and the presence of MDR bacteria, which pose a potential threat to individuals in these areas, is alarming. In addition, detection of integrons could be a good marker for the identification of MDR isolates. Lastly, this study could develop strategies for the proper management of farming sites leading to the detection of food-borne pathogens and prevention of infectious diseases. 相似文献
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This study investigates faecal indicator bacteria (FIB), multiple antibiotic resistant (MAR), and antibiotic resistance genes (ARGs), of sediment profiles from different parts of Lake Geneva (Switzerland) over the last decades. MARs consist to expose culturable Escherichia coli (EC) and Enterococcus (ENT) to mixed five antibiotics including Ampicillin, Tetracycline, Amoxicillin, Chloramphenicol and Erythromycin. Culture-independent is performed to assess the distribution of ARGs responsible for, β-lactams (blaTEM; Amoxicillin/Ampicillin), Streptomycin/Spectinomycin (aadA), Tetracycline (tet) Chloramphenicol (cmlA) and Vancomycin (van). Bacterial cultures reveal that in the sediments deposited following eutrophication of Lake Geneva in the 1970s, the percentage of MARs to five antibiotics varied from 0.12% to 4.6% and 0.016% to 11.6% of total culturable EC and ENT, respectively. In these organic-rich bacteria-contaminated sediments, the blaTEM resistant of FIB varied from 22% to 48% and 16% to 37% for EC and ENT respectively, whereas the positive PCR assays responsible for tested ARGs were observed for EC, ENT, and total DNA from all samples. The aadA resistance gene was amplified for all the sediment samples, including those not influenced by WWTP effluent water. Our results demonstrate that bacteria MARs and ARGs highly increased in the sediments contaminated with WWTP effluent following the cultural eutrophication of Lake Geneva. Hence, the human-induced changing limnological conditions highly enhanced the sediment microbial activity, and therein the spreading of antibiotic resistant bacteria and genes in this aquatic environment used to supply drinking water in a highly populated area. Furthermore, the presence of the antibiotic resistance gene aadA in all the studied samples points out a regional dissemination of this emerging contaminant in freshwater sediments since at least the late nineteenth century. 相似文献
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如何合理确定村镇供水管网敷设方案,关系到整个供水系统的安全性和可靠性。通过生成树变化法,将环状管网转化成树状管网,确定供水管网中最短供水路线,再计算最短供水路线下的最低综合费用、供水系统的经济管径,以确定最优管道敷设方案。根据该方法对四川省某镇供水系统进行了设计。 相似文献
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将产业结构演变的城市化响应系数作为衡量区域产业结构演变城市化响应强度的变量指标,运用变异系数和离差分析方法对陕西省产业结构演变的城市化响应进行类型划分,可划分为强响应区、中等响应区、弱响应区三种类型区;运用广义脉冲响应法对三种类型区分别进行产业结构演变城市化响应系数与各影响因素之间相关性的动态研究,探讨陕西省产业结构演变的城市化响应时至差异性。 相似文献
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以糖、淀粉、蛋白质、纤维素培养基及菌株增殖倍数、菌株性能等为筛选方法,从牛粪低温堆肥中筛选不同原料重要功能菌并组成功能菌剂;以不同类别原料菌及单菌株发酵实验对升温机制进行研究。结果表明,芽孢杆菌、假单胞菌、纤维单胞菌及小孢霉菌等为重要功能菌;菌剂起温快,升温效果好,48 h堆体即由低温进入中温,5 d达到高温,腐熟时间可缩短至约17~18 d;糖功能菌升温效果最为显著,淀粉及蛋白质功能菌次之,纤维素功能菌升温能力较弱;菌株利用各种原料能力越强,增殖速度越快,对升温作用越大。各菌株充分利用基质各成分,紧密协同和促进,是低温堆体发酵升温的主要作用机制。 相似文献
119.
把山地环境划分为4种类型,并对各自的特点进行了分析.从导电机理和降阻机理两个方面分析了化学降阻剂的降阻功能,根据不同地质条件讨论了化学降阻剂的合理选择,并对化学降阻剂的应用方法和技术进行了研究,为山区防雷提供一定的参考价值. 相似文献
120.
介绍了接地的目的和种类,针对大型测控设备对接地电阻值的要求,提出了区域面积受限的接地系统设计方案,并对接地电阻进行了详细分析、计算和比较,选出了合理的接地方案进行施工,竣工后对接地系统的接地电阻值进行检测,检测结果满足测控设备接地电阻的要求. 相似文献