ClO2消毒工艺对污水处理厂出水超级耐药基因的影响

为了研究二氧化氯（chlorine dioxide,ClO₂）消毒工艺对污水处理厂出水中超级耐药基因（super antibiotic resistance genes,SARGs）的去除效果,对污水处理厂出水消毒前后的水样进行了全年采集,并采用微孔滤膜正压过滤法及核酸吸附柱-洗脱法分别富集水中细菌和胞外核酸后,利用荧光定量PCR技术对其中的9种SARGs进行定量检测.结果表明,无论是胞内还是胞外核酸,均有NDM-1、MCR-1 和MEC-A被检测出;同时,ClO₂消毒后上述3种SARGs的胞内相对总浓度明显上升（P<0.05）,且ClO₂消毒对胞内SARGs相对浓度的影响与季节有关,其中春季、夏季和秋季均发生上升,且春季升高最为明显,达2倍,而冬季出水在消毒前后均未测出胞内SARGs;胞外SARGs则在ClO₂消毒前后没有明显浓度变化.因此,ClO₂消毒不能有效去除污水处理厂出水中胞内和胞外SARGs污染.     

上海沙田湖养殖区及周边水体中氟喹诺酮类抗性基因的分布特征及其与环境因子关系

集约化水产养殖区常被认为是水体抗生素和抗性基因的主要潜在来源,然而对其该类水体中抗生素和抗性基因污染情况的研究仍相对匮乏.本文以上海市沙田湖养殖区及周边水体为调查对象,利用宏基因组学高通量测序技术,于2020年9月对水体中的氟喹诺酮类抗生素(fluoroquinolones,FQs)和氟喹诺酮类抗性基因(fluoroquinolones antibiotic resistance genes,FQs-ARGs)的种类和丰度进行了调查,并利用多元统计分析方法研究了水体理化因子和FQs对FQs-ARGs的影响.结果表明,沙田湖养殖区及周边水体共检测出5类耐药机制、共46种FQs-ARGs,其中各水体共有基因为44种.抗性基因mfd和patA在养殖水体和周边水体中均为优势抗性基因,抗生素抗性基因变异或突变为优势耐药机制.养殖水体和周边河湖水体、不同养殖品种水体之间FQs-ARGs的平均丰度无显著差异(P＞0.05).偏冗余分析(pRDA)表明,除了诺氟沙星和环丙沙星2种抗生素外,水体电导率(Spc)、总磷(TP)、总氮(TN)、氨氮(NH4+-N)、溶解氧(DO)、pH和叶绿素a(Chl-a)等环境因子是影响水体中FQs-ARGs组成与分布的关键性因子,且非抗生素因子的总贡献率远高于抗生素.     

石家庄市土壤中喹诺酮类抗生素时空分布及其风险评估

由于喹诺酮类(QNs)药物在人类医学中的重要性,世界卫生组织将其列为“最重要的抗菌药物”.鉴于此,为阐明土壤中喹诺酮类抗生素时空分布特征及其风险,分别于2020年9月(秋季)和2021年6月(夏季)采集了18份表层土壤样品,并采用高效液相色谱串联质谱法(HPLC-MS/MS)分析测定了土壤样品中的QNs抗生素含量,明晰了QNs时空分布特征及其环境影响因子;并采用风险商值法(RQ)进行了QNs生态风险和抗性风险评估.结果表明：(1)由2020年9月(秋季)至2021年6月(夏季), QNs含量平均值呈现下降趋势[秋季和夏季ω(QNs)平均值分别为94.88μg·kg^-1和44.46μg·kg^-1];中部(S9～S15)土壤中QNs含量最高而其他区域较低;(2)土壤中粉粒平均占比并无显著变化,而黏粒和砂粒平均占比分别呈升高和下降趋势;总磷(TP)、氨氮(NH⁺₄-N)和硝氮(NO^-₃-N)含量平均值呈下降趋势;(3)相关分析结果表明,QNs含量与土壤粒径、亚硝...     

Denaturing high-performance liquid chromatography (DHPLC)-based prenatal diagnosis for tuberous sclerosis

Tuberous sclerosis (TSC) is a frequent autosomal-dominant condition (affecting 1 in 6000 individuals) caused by various mutations in either the hamartin (TSC1) or the tuberin gene (TSC2). This allelic and non-allelic heterogeneity makes genetic counseling and prenatal diagnosis difficult, especially as a significant proportion of TSC cases are due to de novo mutations. For this reason the identification of the disease causing mutation is mandatory for accurate counseling, yet current mutation detection methods such as single-strand conformation polymorphism (SSCP) or denaturing gradient gel electrophoresis (DGGE) are labor intensive with limited detection efficiency. Denaturing high-performance liquid chromatography (DHPLC) is a high-throughput, semi-automated mutation detection system with a reported mutation detection rate close to 100% for PCR fragments of up to 800 bp. We used a recently described DHPLC assay allowing the efficient detection of mutations in TSC1 to analyze the DNA extracted from a chorion villus sample in order to perform a prenatal diagnosis for TSC. The fetus was found not to have inherited the deleterious mutation and the DHPLC diagnosis was confirmed by haplotype analysis. This represents the first DHPLC-based prenatal diagnosis of a genetic disease. Copyright © 2001 John Wiley & Sons, Ltd.     

The spatiotemporal structure of population in the Muskrat (Ondatra zibethicus)

The spatiotemporal structure of the muskrat population has been studied by means of the prenatal radioactive marking of juveniles with 45 Ca and their subsequent recapture in the test area. The muskrat population is characterized by a cyclic type of spatial structure, with seasonal transition from a mosaic type of settling to a diffuse type and, after the breeding season, vice versa.     

广东土地抗蚀潜势初步研究

利用计算机和地理信息系统软件．研究了广东省的土壤质地、土壤有机质、地势三个侵蚀因子对土壤侵蚀方面的影响，指出了广东土地抗蚀性极弱地区主要是湛江市、新丰县的小部分地区，而抗蚀性弱的区域则很大，约占全省土地面积的49．8％，遍布全省中部和南部．针对这样的现状，提出要减少抗蚀性弱和极弱地区的土壤侵蚀，必须做到：对土地的利用开垦进行统一规划；严禁乱伐森林；采取生物措施和工程措施相结合的方式防治土壤侵蚀的进一步变化．     

振动加载下转换型密封电磁继电器的性能退化行为研究

目的研究航空用继电器的失效机理。方法通过对电磁继电器进行振动与工作载荷单独施加以及二者综合加载情况下的试验方法及性能退化行为研究,实时监测继电器内部触点间接触电阻等性能参数的变化趋势,并对不同条件下触点表面的微观形貌进行对比分析。结果发现相比较工作载荷单独施加而言,振动应力的加入使得继电器的寿命大幅度降低,并使得触点表面呈现"雪花"一般的形貌。结论振动环境导致了触点之间电弧的变化,以及触点表面接触压力、接触面积和接触超程等,并通过燃弧时间和超程时间的变化趋势对其进行了验证。     

The characteristic of in situ stress in outburst area of China

In order to obtain the characteristics of in situ stress field in outburst mining area, using the hollow inclusion (HI) technique to measure the in situ stress of coal and gas outburst mining area. The measurement sites located in northeast, middle and east of china, which include eight mining areas such as Fuxin, Pingdingshan, Hebi and Huainan. Base on the analysis of measurement data from outburst mining area, conclusions could be obtained as follow: (a) major principal stress and minor principal stress is horizontal stress and interim principal stress is vertical stress, and the type of outburst area’s stress field is dynamic stress field; (b) the major principal stress and minor principal stress are higher than other regions and the tectonic stress is outstanding; and (c) the ratio of major principal and vertical stress is decrease with the increase of depth and the type of stress field is likely transfer dynamic to static. Thus, in situ stress plays a key role during the occurring and development of coal and gas outburst, which is an important reason of severe outburst in China.     

液压支架薄煤层采煤工作面风流流动规律研究

分别对北宿煤矿以装备液压支架和单体支柱为主的14710和1667薄煤层采煤工作面风流场进行了现场测定研究,结果显示,装备液压支架后,工作面的风流流动规律发生了较大变化,百米风阻值及向采空区的漏风率均大幅度提高。     

基于时变失效的桥式起重机结构可靠性分析

为提高我国起重机产品的安全可靠性水平,用时变可靠性理论分析桥式起重机金属结构。以桥式起重机金属结构为研究对象,时变可靠性理论为基础,研究桥式起重机金属结构的载荷时变可靠性模型和抗力时变可靠性模型,建立桥式起重机金属结构失效状态的时变可靠性数学模型。通过工程算例分析,揭示金属结构抗力随时间衰减与否对可靠度与失效概率的影响,得到不同金属结构抗力衰减系数下桥式起重机主梁金属结构可靠度和失效概率。探索桥式起重机金属结构时变可靠性安全评估方法。对比金属结构抗力衰减系数分别为0.000 2和0.000 3时的分析结果,发现金属结构系统的可靠性随金属结构抗力衰减系数的增加而降低。