Sensitive voltammetric and amperometric responses of respiratory toxins at hemin-adsorbed carbon-felt

A hemin [iron-Fe(III) protoporphyrin IX chloride] was adsorbed onto a carbon-felt (CF), which is a microelectrode ensemble of micro carbon fiber (ca. 7 μm diameter). The resulting hemin-adsorbed-CF (hemin-CF) showed a well-defined redox wave based on the hemin-Fe(III)/Fe(II) redox process with the formal potential of -0.225 V vs. Ag/AgCl in deoxygenated phosphate/citrate buffer solution (0.1 mol/L, pH 5.0). The apparent heterogenous electron transfer rate constant was estimated to be 8.6 sec^-1. In air-saturated electrolyte solution, the hemin-CF exhibited an excellent electrocatalytic activity for the reduction of dioxygen (O₂). This activity was reversibly inhibited by respiratory toxins such as cyanide and azide, which bind sixth coordination position of iron active center of hemin. The electrocatalytic O₂ reduction current at the hemin-CF was modulated by the toxins in a concentration-depending manner. Based on the relationship between the inhibition and the toxin concentration, apparent inhibition constants of cyanide and azide were evaluated to be 4.52 and 1.98 μmol/L, respectively. When the hemin-CF was used as a working electrode unit of the CF-based electrochemical flow-through detector with air-saturated carrier, the injection of the azide induced peak-shape current responses, which allowed rapid and continuous flow-amperometric determination of azide with high sensitivity.     

防冲吸能构件应变特性及巷道支架应用研究*

为明确防冲吸能支护的力学特性及其应用效果,基于竖向准静态压缩试验研究防冲吸能构件的荷载位移曲线,采用ABAQUS模拟防冲吸能构件的变形压溃过程,并与试验结果进行对比,对变形过程中构件管壁的应变演化进行分析,进而研究吸能支架在冲击作用下的应变特性。研究结果表明:数值计算可以较好地对防冲吸能构件的变形吸能过程和荷载位移曲线进行模拟;采用该构件的液压支架能够满足防冲吸能支护的设计理念和原则;对吸能支架吸能特性研究,防冲吸能构件能够在冲击来临时更好地保护支架,研究成果可为冲击地压巷道支护体系提供设计参考。     

Prenatal diagnosis of respiratory chain deficiency by direct mutation screening

Respiratory chain deficiency (RCD) is responsible for a clinically heterogeneous group of early-onset untreatable disorders. Enzymological prenatal diagnosis (PD) can only be offered to a fraction of families. Moreover, due to the two-fold genetic origin of the respiratory chain (nuclear and mitochondrial DNA) and owing to the large number of nuclear genes involved in the respiratory chain assembly, maintenance and functioning, the identification of the disease causing gene in a given family remains challenging. Here, we report on PD of RCD by direct screening of NDUFV1, SDH-Fp, SCO1 and SURF1 mutations in five unrelated families with complex I, II and IV deficiency, respectively. The identification of the disease-causing gene in a given family with RCD is a major issue to provide both adequate genetic counselling and early, reliable PD. Copyright © 2001 John Wiley & Sons, Ltd.     

某铅锌矿采空区处理与卸压开采方案研究

900m水平似上采空区回收矿柱及900 m水平以下的巷道掘进或矿房开采时经常发生岩爆.为了安全、经济地回收矿柱并实施深部开采,在数值分析的基础上,提出了一种急倾斜矿体的采空区处理与卸压开采方法,并用ANSYS数值模拟确定了采空区处理及卸压开采的施工参数.结果表明,在下盘脉外巷道离采空区边缘的水平距离为10 m时,上盘脉外巷道离采空区边缘的水平距离取20m;在巷道底板隔断开采的深度不超过20m;必须从上、下盘脉外巷道同时向采空区围岩实施V型松动爆破;可只在上盘巷道底板进行隔断开采.该采空区处理与卸压开采方法技术可行、经济合理、简便适用.当地表允许岩体移动时这种新方法适合急倾斜、厚度大矿体的采空区处理与卸压开采;如果地表不许可岩体移动,使用这种新方法是不合适的.该采空区处理与卸压开采方法是一种采空区处理的联合法.它能够用工程爆破这一种手段实现采空区处理、应力向有利于安全生产的方向转移等多种目的.     

7种酚类化学物质对活性污泥的呼吸抑制作用

苯酚类化学物质是废水中常见的有机污染物,其对活性污泥的毒性数据对于污水处理厂稳定运行和化学品危害性评估具有重要意义。本研究采用活性污泥呼吸抑制试验(209)测定7种酚类化学物质对活性污泥的呼吸抑制作用。结果显示,2,6-二叔丁基苯酚和对特辛基苯酚对活性污泥未产生明显的毒性效应;2,4-二氯酚、2-苯基苯酚、4-硝基酚、4-氯酚和对甲酚等5种化学物质都对活性污泥呼吸有不同程度的抑制效应,3 h-EC50值分别为49.7、77.6、102、150.1和462 mg·L-1,构效关系分析结果表明-Cl、-NO2等官能团是导致活性污泥呼吸抑制效应增强的关键因素。在化学品生物降解性测试研究中,要确保有毒化学物质的测试浓度低于EC50值的1/10。     

沉积物中重金属Pb和Cd对河蚬的毒性效应研究

以河蚬为受试生物,以存活率、呼吸抑制率为测试终点,研究了沉积物和上覆水中重金属Pb、Cd对底栖生物的毒性效应,并探讨了沉积物中酸挥发性硫化物(acid volatile sulfide,AVS)和同时提取金属(simultaneously extracted metals,SEM)对重金属生物有效性的影响。结果表明,沉积物中Pb、Cd对河蚬致死效应较低,当Pb、Cd浓度分别为400、100 mg獉kg-1,连续暴露21 d时,致死率低于20%;沉积物中Pb、Cd对河蚬14 d的呼吸抑制率EC50分别为519和151 mg獉kg-1;上覆水中Pb和Cd离子浓度较低,对生物毒性效应贡献可以忽略;SEM/AVS值和生物呼吸抑制率有着明显的线性相关性,当SEMPb/AVS1,SEMCd/AVS0.6时,重金属对河蚬有明显的毒性效应。     

液化石油气钢瓶异常破口原因分析

YSP23．5液化石油气钢瓶爆破失效结果不符合要求这一实例入手，针对钢瓶材质、自身结构设计等主要因素，对钢瓶热处理工艺和机加工工艺中等几个值得注意的环节及异常破口的原因进行了综合解析，为立式单环焊缝液化石油气钢瓶设计思路及制造工艺提出改进建议。     

不同卸荷速率条件下岩爆碎屑破坏特征分析

为研究卸荷速率对岩爆的影响,采用岩石真三轴伺服诱变试验系统对大理岩进行不同卸荷速率下的岩爆试验,收集试验后得到的碎屑。通过对碎屑质量、粒径以及基本尺度的测量,得到碎屑的尺度特征以及不同粒径质量的分布特征;同时对所得到的碎屑进行分形特征研究。结果表明:在岩爆过程中,存在大量破碎的块状、板状、片状以及薄片状碎屑携带大量动能向临空面崩落,其携带的动能是由岩体自身储存的能量转化而来;卸载速率对岩爆的剧烈程度有着直接的影响,卸荷速率越快,发生岩爆时其程度越剧烈。     

呼吸防护用品的人机工效学问题分析

呼吸防护用品符合人的形态与生理特征,才能保证其防护效果和舒适性。笔者从人体的面部形态与生理机能角度,分析了呼吸防护用品与人体的适配性,系统提出密合性、呼吸阻力、死腔、视野等呼吸防护用品的人机工效学问题。该论文取得的成果,对进一步丰富呼吸防护用品人机工效学研究内容,指导呼吸防护用品的设计与生产,提升我国呼吸防护用品的防护效果与核心竞争力,以期发挥积极的推动作用。