QuEChERS-超高效液相色谱-串联质谱法测定鱼血清中的精神活性物质

本文建立了一种同时测定鱼血清中19种精神活性物质的分析方法.该方法基于QuEChERS方法对样品进行提取和净化,待测物采用超高效液相色谱-串联质谱法(UPLC-MS/MS)进行分析,检测的19种精神活性物质均采用同位素内标法定量.该方法的方法检测限(MDL)范围为0.02-0.06 ng.mE-1,基质效应处于76.7...     

机载电子元器件环境应力筛选初探

讨论了某直升机机载电子元器件在环境应力筛选过程中存在的主要问题及其解决方法。包括环境应力筛选的实施原则,筛选项目的确定,失效元器件分析情况及失效元器件的处理等问题。     

镉污染对莫桑比克罗非鱼血清蛋白的影响

通过分析莫桑比克罗非鱼血清蛋白含量的变化,研究镉对鱼类的致毒效应,试验结果表明,镉能升高血清总蛋白,球蛋白含量、降低白蛋白／球蛋白比值,但对血清白蛋白含量没有影响,镉对血清球蛋白各组分的影响有其特异性,对莫桑比克罗非鱼血清球蛋白成分产生作用的镉浓度阈值为０．００５ｍｇ／Ｌ,分析血清蛋白含量的变化,可以为诊断镉污染提供理论依据。     

Improving Efficiency Of Uncertainty Analysis In Complex Integrated Assessment Models: The Case Of The Rains Emission Module

Abstact Ever since the Regional Acidification Information and Simulation Model (RAINS) has been constructed, the treatment of uncertainty has remained an issue of major interest. In a recent review of the model performed for the Clean Air for Europe (CAFE) programme of the European Commission, a more systematic and structured uncertainty analysis has been recommended. This paper aims at contributing to the scientific debate how this can be achieved. Because of its complex structure on the one hand and limited research resources (time, computational capacities) on the other hand a full-blown uncertainty analysis in RAINS is hardly feasible. Therefore, all types of uncertainty require more efficient ways for uncertainty analysis. With respect to parameter uncertainty, we propose to focus research efforts for uncertainty analysis on key parameters. Among different approaches to select key parameters that have been discussed in the literature screening methods seem to be particularly appropriate for complex, deterministic Integrated Assessment models such as RAINS. Surprisingly, in Integrated Assessment modelling for air pollution problems of screening design have not been taken up so far. As a case study we consider the emission module of RAINS. We show that its structure allows for a straightforward and effective screening procedure     

Evaluation of fungal potentiality for bioconversion of domestic wastewater sludge

This study was undertaken to screen the filamentous fungi isolated from its relevant habitats (wastewater, sewage sludge and sludge cake) for the bioconversion of domestic wastewater sludge. A total of 35 fungal strains were tested against wastewater sludge (total suspended solids, TSS 1%-5% w/w) to evaluate its potentiality for enhancing the biodegradability and dewaterability using liquid state bioconversion(LSB) process. The strains were divided into five groups i. e. Penicillium, Aspergillus, Trichoderma, Basidiomycete and Miscellaneous, respectively. The strains WWZP1003, SCahmA103, SCahmT105 and PC-9 among their respective groups of Penicillium,Aspergillus, Trichoderma and Basidiomycete played potential roles in terms of separation (formation of pellets/flocs/filaments),biodegradation(removal of COD) and filtration(filterability) of treated domestic wastewater sludge. The Miscellaneous group was not considered due to its unsatisfactory results as compared to the other groups. The pH value was also influenced by the microbial treatment during fermentation process. The filterability of treated sludge was improved by fungal treatment, and lowest filtration time was recorded for the strain WWZP1003 and SCahmA103 of Penicillium and Aspergillus groups respectively compared with other strains.     

基于风险分析的流域优先有机污染物筛查:研究与应用

应用高通量分析方法对大清河流域47个沉积物中的潜在风险有机污染物进行了分析,共筛查到104种有毒有机污染物。应用基于风险分析的筛查体系对检出率高于10%的54种污染物进行了概率风险评估,筛查出11种具有潜在风险的流域优先有机污染物,包括农药(特草克、氨磺磷、枯莠隆)、工业用品及副产物(二苯胺、9,10-蒽醌、萘、2-甲基萘)及家庭及个人护理品(咖啡因、苯乙酮、苯甲酮、联苯)等。结果表明,基于风险分析的流域污染物筛查方法体系可成功地对流域内优先污染物进行识别与筛查,为流域管理提供科学依据。     

呼吸道感染患儿血清微量元素的测定及其与抗体水平的关系

应用流动注射氢化物发生原子吸收光谱法（HGAAS）测定了呼吸道感染（RTI）患儿血清中Se和Pb,火焰原子吸收光谱法（AAS）测定Fe、Cu、Zn、Ca、Mg及Mn,光度法测定了P,以标准牛血清作质控,在最佳实验条件下,9种元素的精密度,检出限以及回收率均令人满意,根据患儿IgG,IgA,IgM补体C3及C4的测定结果,对测定方法及呼吸道感染与血清微量元素和抗体水平的关系进行了探讨,发现RTI患儿血清Se、Fe、Zn、Mn、Ca明显低于同龄正常儿童,且Ig、C3均与CK存在显著性差异。认为对RIT患儿有必要监测其血清微量元素水平。     

Bacteria Associated with Degradation of Wastes from Rubber Processing Industry

An investigation of bacteria found in rubber processing wastes was carried out. Rubber wastes which include effluents from washing tanks and natural rubber waste serum (NRWS) were obtained from Greenpark Rubber Industries Limited, Umutu, Delta State, Nigeria. Five bacterial species were isolated from the wastes. These include Arthrobacter sp., Bacillus sp., Lactobacillus sp., Psuedomonas sp. and Streptococcus sp. Apart from these a number of coliforms were also encountered. Arthrobacter sp. was found to be the dominant species and its potential to utilize rubber hydrocarbon was determined. It was found that the growth of Arthrobacter in both effluent and NRWS was related to pH with the highest growths recorded at pH of 8.5 and 7.5 for effluent and NRWS respectively. It was also found that at controlled pH of 7.5 in NRWS, the growth of Arthrobacter was consistent and was accompanied by a reduction in biological oxygen demand (BOD) which was the the main index for measuring pollution strength of the wastes. It is therefore being recommended that rubber wastes be treated with Arthrobacter under controlled pH to reduce their pollution potentials before disposal. It is however suggested that a combined biological treatment using both Arthrobacter and Mucor as was earlier suggested be used. It is also recommended that chemical flocculants should be used to remove suspended solids in the effluent. A combination of these two cheap methods will go a long way in alleviating the problems of pollution caused by rubber effluents from some tropical rubber processing factories.     

Interaction of tebuconazole with bovine serum albumin: determination of the binding mechanism and binding site by spectroscopic methods

Abstract

This study investigates the interaction between tebuconazole and bovine serum albumin (BSA) in a physiological buffer (pH = 7.4) using the fluorescence quenching method to obtain the apparent binding constants (K) and number of binding sites (n) in the interaction between tebuconazole and BSA. The results revealed that tebuconazole can quench the intrinsic fluorescence of BSA through a static quenching procedure. It also shows that the thermodynamic parameters of enthalpy change (ΔH) and entropy change (ΔS) are negative, indicating that the interaction of tebuconazole with BSA is mainly driven by van der Waals forces and hydrogen bonds. The process of binding was a spontaneous process in which Gibbs free energy change was negative. The distance of r between the donor (BSA) and acceptor (tebuconazole) was calculated to be 0.68?nm based on Forster’s non-radiative energy transfer theory. Analysis of synchronous fluorescence, three-dimensional fluorescence and circular dichroism (CD) spectra demonstrates that tebuconazole can induce conformational changes of BSA.     

一株高浸磷嗜酸氧化硫硫杆菌的分离与鉴定

从矿山土样中分离到一株嗜酸的浸磷矿细菌HY-01,观察其形态并研究其培养特征.结果显示,该菌株为革兰氏阴性、短杆状运动细菌,菌体长1～2μm,宽0.5～0.7 μm,能在pH 1.5～8.0的范围内生长,最适生长温度为37℃,最适pH为1.5～3.0.该菌株可以KNO3、NH4NO3、(NH4)2CO3、NH4H2PO...