Reproductive plasticity in bumblebee workers (<Emphasis Type="Italic">Bombus terrestris</Emphasis>)—reversion from fertility to sterility under queen influence

Worker sterility in the bumblebee Bombus terrestris is conditional and is linked to the social development of the colony. Workers refrain from reproducing or overtly challenging the queen until gyne production has initiated, at the so-called competition point (CP). It is not known whether this behavior is hard-wired or workers show reproductive plasticity. It also remains unclear whether worker reproductive decision is under queen and/or worker control. In this study, we tested worker reproductive plasticity in an attempt to assess whether and under which conditions worker sterility/fertility are reversible. We introduced egg-laying workers into colonies with different social structures for 1 week then monitored their reproductive status. We revealed a remarkable reproductive plasticity in the introduced workers that was social-condition-dependent. In the presence of a pre-CP queen, the introduced workers reverted to sterility, whereas in the presence of a post-CP queen, such workers remained egg-layer. Reversion to sterility does not occur when direct contact with the queen is prevented, as the introduced workers remained egg-layer in the queenright colonies with a confined queen. Egg-laying workers that were introduced into queenless colonies mostly maintained their fertility regardless of colony social phase. This shows that worker transition from cooperative to selfish behavior is reversible depending on the social context.     

木聚糖酶基因xynB在不同大肠杆菌表达系统中的表达比较

从黑曲霉(Aspergillus niger)nl-1中克隆获得木聚糖酶基因xynB.该基因全长745 bp,含有67 bp内含子,与公布的黑曲霉xynB基因有较高的同源性.将PCR扩增的木聚糖酶成熟肽基因和含有信号肽的基因分别连接到表达载体肠杆菌Top10 F’、DH10B和两种BL21宿主中获得重组菌株.通过IPTG诱导,xynB基因在重组菌株中获得特异性表达.表达产物以胞内可溶性蛋白和不溶性包涵体形式存在.诱导4 h,重组菌株pTrc-99a-xynB[BL21 condon plus(DE3)]表达量最高,胞内酶活达到299 IU/L.重组质粒pTrc-99a-xyn B(S)在不同宿主胞内和胞外均能分泌目的蛋白,诱导10 h,胞外酶活pTrc-99a-xynB(S)[BL21 condon plus(DE3)]达到347 IU/L.而pET-20b-xynB(S)在两种BL21宿主中均不表达.经SDS-PAGE分析,以pTrc-99a和pET-20b作为表达载体时,重组蛋白相对分子质量分别约为45×103和30×103.与pET-20b相比,pTrc-99a以及自身信号肽的引导更有利于重组木聚糖酶的表达.     

基于条件变分自编码器的齿轮箱故障诊断

目的实现齿轮箱故障类型的智能识别诊断。方法针对传统故障诊断方法通用性不广、数据依赖强、泛化能力弱并需人工提取特征问题,提出一种基于条件变分自编码器的故障诊断方法。以故障类别概率分布为目标并将振动信号频谱作为条件,通过条件变分自编码器,建立齿轮箱振动信号频谱到对应各故障下的条件概率模型,并通过多层神经网络结合变分推断方法进行训练优化,实现对齿轮箱各类型故障的高精度分类诊断。结果在仅有少量训练数据条件下,实现了准确的故障识别。结论条件变分自编码器在齿轮箱振动信号频谱概率分布建模上具有优异性能,对故障信号数据量的依赖低、泛化能力强,无需人工提取特征。能有效实现齿轮箱故障的智能分类诊断。     

爆炸分离冲击数据的零漂校正

目的为消除爆炸分离冲击信号中的零漂趋势项,提出一种爆炸分离冲击数据处理方法。方法基于分段和线性拟合思想,获取测试数据的零漂趋势项。通过分析原始信号和校正信号的频谱相关性,定量讨论分段窗口数对试验数据有效性的影响规律,进而给出爆炸分离冲击加速度信号的校正原则。结果通过对试验数据进行有效性分析与校正,能够较好地抑制积分零漂,消除基线零漂趋势项对冲击谱的影响。结论该方法可提高试验数据的准确性和有效性。     

两栖装备入舰引导系统方案研究

目的针对两栖装备上下登陆舰时自动化程度低、无法全天候实施的问题,提高恶劣海况和黑夜条件下的登舰作业能力,提出一种两栖装备入舰引导系统设计方案。方法依托装备再制造升级技术手段,通过水下探测器接收登陆舰发射的超声波定位脉冲信号,获得装备相对位置和路线规划信息,有效规避航线水下障碍物,提高装备登舰的准确度和效率。结果针对系统发出的超声波信号部分被水下超声接收器接收的情况,对海水、海洋环境噪声和海洋混响等因素对声波信号传输特性的影响展开研究,重点分析了浅海海域的季节性声速剖面、传播过程中的声强损失,对海洋环境噪声、海底和海面的等效平面波混响级进行数学建模,通过静态和动态试验,验证了模型推导的准确性和数据的可靠性。结论通过浅海多径传播信号合成模型和双路径传播的信号衰减模式的推导,为求解声波传播模型的波动方程奠定了基础。     

Empirical Research on Factors Influencing Level of Environmental Protection Information Disclosure in Annual Reports by Listed Companies

Taking 248 A-share manufacturing listed companies in 2006 Shanghai Stock Exchange as example, using ＂index method＂ to measure level of environmental protection information disclosure, this paper examines the factors influencing level of environmental protection information disclosure. The findings show that the disclosure level has improved, but the overall level is still low and unable to satisfy information users; there is positive correlation between corporate size, industry type, accounting firm＇s type and corporate environmental disclosure index. The companies of large scale and heavy pollution and those audited by international ＂Big 4＂ and domestic ＂Big 10＂ accounting firms have a high level of environmental disclosure, while the profitability and financial leverage has no impact on the disclosure level. Finally some suggestions are put forward to establish environmental information disclosure system in China.     

基于短时傅里叶变换的飞行器故障振动信号分析

目的 对某飞行器出现故障时的振动信号进行分析,为故障定位提供依据.方法 将短时傅里叶变换方法 应用于飞行振动信号分析,通过计算故障振动信号和正常振动信号的短时傅立叶变换谱,获取两者之间的不同时频特征.通过分析包括发动机工作、外部气动激励、设备工作、电气干扰在内的各种影响因素的时频变化特征,与故障振动信号的时频特征对比,...     

基于S变换的微地震监测中多源信号特征分析

为了提高微地震信号分析的精度,对模拟振动信号进行了试验,得到了时窗因子对ST(S-Transform)时频分辨率的影响,基于ST的良好时窗自适应性和高时频分辨率,进一步将其应用于泸沽铁矿巷道微地震监测数据的处理。结果表明:ST能准确提取信号属性用以构造特征向量,实现了矿山微地震监测中多源信号的量化分类,并为矿山微地震信号的自动识别与分析提出了新思路。     

我国道路交通信号控制机认证框架体系探讨

针对目前我国道路交通信号控制机产品存在的质量问题,对信号机生产企业进行深入调研,详细分析信号机发展现状、国内外产品认证制度及信号机强制检测制度等,并结合我国交通信号机产品自身的特点和实际,参考国外产品认证模式、认证流程,提出我国信号机产品以型式试验、初始工厂检查和获证后监督为模式的认证框架体系。信号机产品质量认证框架体系的建立,对提高产品质量,规范行业生产,将发挥积极的促进作用,同时也为我国交通安全产品的质量控制提供了新思路。     

Fish forewarning of comprehensive toxicity in water environment based on Bayesian sequential method

Environmental impact of pollutants can be analyzed effectively by acquiring fish behavioral signals in water with biological behavior sensors. However, a variety of factors, such as the complexity of biological organisms themselves, the device error and the environmental noise, may compromise the accuracy and timeliness of model predictions. The current methods lack prior knowledge about the fish behavioral signals corresponding to characteristic pollutants, and in the event of a pollutant invasion, the fish behavioral signals are poorly discriminated. Therefore, we propose a novel method based on Bayesian sequential, which utilizes multi-channel prior knowledge to calculate the outlier sequence based on wavelet feature followed by calculating the anomaly probability of observed values. Furthermore, the relationship between the anomaly probability and toxicity is analyzed in order to achieve forewarning effectively. At last, our algorithm for fish toxicity detection is verified by integrating the data on laboratory acceptance of characteristic pollutants. The results show that only one false positive occurred in the six experiments, the present algorithm is effective in suppressing false positives and negatives, which increases the reliability of toxicity detections, and thereby has certain applicability and universality in engineering applications.