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Stin OC Carnahan A Singh R Powell J Furuno JP Dorsey A Silbergeld E Williams HN Morris JG 《Environmental monitoring and assessment》2003,81(1-3):327-336
Molecular methods, including DNA probes, were used to identify and enumerate pathogenic Vibrio species in the Chesapeake Bay; our data indicated that Vibrio vulnificus exhibits seasonal fluctuations in number. Our work included a characterization of total microbial communities from the Bay; development of microarrays that identify and quantify the diversity of those communities; and observation of temporal changes in those communities. To identify members of the microbial community, we amplified the 16S rDNA gene from community DNA isolated from a biofilm sample collected from the Chesapeake Bay in February, 2000. The resultant 75 sequences were 95% or more similar to 7 species including two recently described Shewanella species, baltica and frigidimarina, that have not been previously isolated from the Chesapeake. When the genera of bacteria from biofilm after culturing are compared to those detected by subcloning amplified 16S fragments from community DNA, the cultured sample exhibited a strong bias. In oysters collected in February, the most common bacteria were previously unknown. Based on our 16S findings, we are developing microarrays to detect these and other microbial species in these estuarine communities. The microarrays will detect each species using four distinct loci, with the multiple loci serving as an internal control. The accuracy of the microarray will be measured using sentinel species such as Aeromonas species, Escherichia coli, and Vibrio vulnificus. Using microarrays, it should be possible to determine the annual fluctuations of bacterial species (culturable and non-culturable, pathogenic and non-pathogenic). The data may be applied to understanding patterns of environmental change; assessing the health of the Bay; and evaluating the risk of human illness associated with exposure to and ingestion of water and shellfish. 相似文献
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生态系统服务的核算方法 总被引:3,自引:0,他引:3
生态系统服务是指对人类生存及生活质量有贡献的生态系统产品和生态系统功能 ,包括由自然生态过程产生并维持的环境和资源条件。目前已有的生态系统服务核算方法可以分为市场价值法、替代市场法和假想市场法。 相似文献
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IntroductionTheuseofchemicals,includingpesticides,hasbecomeanintegralandeconomicallyessentialpartofmodernagriculture.Pesticide?.. 相似文献
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Effects of atmospheric mercury pollution on terrestrial ecosystem in Chongqing, China 总被引:1,自引:0,他引:1
1 IntroductionMercury(Hg)asaglobalpollutanthascausedconcernworldwidesincetheendof 1980s.Itisemittedtotheatmospherefrombothanthropogenicandnaturalsources.Importantsourceoftheformeristhedischargefrom productionanduseofHgaswellasthecombustionoffossilfuels(Nri… 相似文献
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《环境科学学报(英文版)》2023,35(4):174-183
It is the key to control bio-derived dissolved organic matters (DOM) in order to reduce the effluent concentration of wastewater treatment, especially for waste leachate with high organic contaminants. In the present study, the anaerobic degradation of aerobically stabilized DOM was investigated with DOM substrate isolated through electrodialysis. The degradation of bio-derived DOM was confirmed by reduction of 15% of total organic carbon in 100 days. We characterized the molecular behavior of bio-derived DOM by coupling molecular and biological information analysis. Venn based Sankey diagram of mass features showed the transformation of bio-derived DOM mass features. Occurrence frequency analysis divided mass features into six categories so as to distinguish the fates of intermediate metabolites and persistent compounds. Reactivity continuum model and machine learning technologies realized the semi-quantitative determination on the kinetics of DOM mass features in the form of pseudo-first order, and confirmed the reduction of inert mass features. Furthermore, network analysis statistically establish relationship between DOM mass features and microbes to identify the active microbes that are able to utilize bio-derived DOM. This work confirmed the biological technology is still effective in controlling recalcitrant bio-derived DOM during wastewater treatment. 相似文献
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