Direct spectral analysis and determination of high content of carcinogenic bromine in bread using UV pulsed laser induced breakdown spectroscopy

Laser induced breakdown spectroscopy (LIBS) was applied for the detection of carcinogenic elements like bromine in four representative brands of loaf bread samples and the measured bromine concentrations were 352, 157, 451, and 311 ppm, using Br I (827.2 nm) atomic transition line as the finger print atomic transition. Our LIBS system is equipped with a pulsed laser of wavelength 266 nm with energy 25 mJ pulse^?1, 8 ns pulse duration, 20 Hz repetition rate, and a gated ICCD camera. The LIBS system was calibrated with the standards of known concentrations in the sample (bread) matrix and such plot is linear in 20–500 ppm range. The capability of our system in terms of limit of detection and relative accuracy with respect to the standard inductively coupled plasma mass spectrometry (ICPMS) technique was evaluated and these values were 5.09 ppm and 0.01–0.05, respectively, which ensures the applicability of our system for Br trace level detection, and LIBS results are in excellent agreement with that of ICPMS results.     

Kinetic studies of endosulfan photochemical degradations by ultraviolet light irradiation in aqueous medium

Kinetic studies of endosulfan photochemical degradation in controlled aqueous systems were carried out by ultraviolet light irradiation at λ = 254 nm. The photolysis of (α + β: 2 + 1) endosulfan, α-endosulfan and β-endosulfan were first-order kinetics. The observed rate constants obtained from linear least-squares analysis of the data were 1 × 10^?4 s^?1; 1 × 10^?4 s^?1; and 2 × 10^?5 s^?1, respectively, and the calculated quantum yields (φ) were 1, 1 and 1.6, respectively. Preliminary differential pulse polarographic (DPP) analysis allowed to observe the possible endosulfan photochemical degradation pathway. This degradation route involves the formation of the endosulfan diol, its transformation to endosulfan ether and finally the ether's complete degradation by observing the potential shifts.     

Effect of Fe (III) on acid degradation of methylparathion

A study was undertaken to determine the transformation kinetic of methylparathion (O, O, -dimethyl O-4 nitrophenylphosphorotioate) in the presence of Fe(III) between pH 2 and 7. The Fe(III) was not electroactive under the conditions used in this study, and polarographic signals were exhibited by methylparathion and main degradation product only. Data suggest that hydrolysis of methylparathion in an acid medium is catalyzed by Fe(III) and the pesticide did not degrade in this medium without this cation. Methylparathion degradation was observed at all the pHs studied and was independent of the predominant chemical form of Fe(III) in the aqueous medium. The reaction was first-order with pH-dependent rate constant (k) values ranging from 3.3 × 10^{? 3} h^{? 1} to 7.0 × 10^{? 3} h^{? 1}. The k values increased as pH decreased, suggesting that Fe(III) acted as an electrophile in the reaction mechanism.     

内陆水体有色溶解有机物的变化特征

有色溶解有机物(CDOM)是水体中的光学活性物质,其光化学变化对水体中碳循环过程具有重要作用。评价分析不同纬度、不同营养状况下内陆水体的CDOM吸收特性,对建立不同水体的固有光学特性数据库具有重要意义。基于太湖、石头口门水库、巢湖和鄱阳湖的现场CDOM吸收系数,分析aCDOM(412)时空变化特征,并基于不同波段拟合光谱斜率S,利用SA/aCDOM(412)判断了研究区CDOM的不同来源。结果表明,不同纬度、不同营养状况的CDOM吸光特性差异显著,高纬度水体的CDOM含量高于低纬度水体,而浮游植物大量生长的富营养化水体的CDOM吸收大于贫瘠营养水平的水体。受藻类的影响,巢湖部分样点的CDOM吸收光谱特征发生改变,在320nm和600nm处有明显的吸收峰;aCDOM(412)与SA呈较好的负相关关系;并且SA/aCDOM(412)可有效判断研究区CDOM的不同来源。     

三峡水库上游流域大洪峰暴雨天气系统分析

选择三峡水库入库流量代表洪水强度,以长江上游组成宜昌洪水来源的岷沱江、嘉陵江、乌江、宜宾-重庆、重庆-万州、万州-宜昌为主要流域;把出现历史前3名大洪峰的1981年、1998年和2010年汛期作为切入点,针对直接诱发3次大洪峰的暴雨过程,利用长江上游六大流域实况面雨量、宜昌流量和水位,以及NCEP数值产品的客观分析等资料,采用天气分析、波谱分析方法,分析了对流层中低层主要天气系统,以及200 hPa南亚高压和地面冷空气的情况。指出了3次大洪峰暴雨的环流特征、风场特征、超长波和长波特征等,为三峡水库气象保障服务提供了洪峰暴雨的天气背景。     

基于谱修正方法的非高斯风场模拟

为克服基于H erm ite谱修正方法的缺点,减少该方法中计算H erm ite多项式系数所需耗费的大量机时,提出了一种模拟非高斯风压场的新方法,采用非高斯累积分布函数(CDF)映射技术来代替基于H erm ite的概率密度函数(PDF)修正。选择任意边缘PDF模型作为概率目标模型,采用目标功率谱密度(PSD)作为样本函数,通过迭代修正该样本函数,使其收敛于目标概率密度函数和目标功率谱密度。将该方法应用于实际结构的非高斯风场模拟,模拟结果与目标谱符合良好,表明本文方法模拟非高斯风场具有较高的精确度和计算效率。     

预应力钢筋混凝土梁破坏过程的声发射特性实验研究

利用全波形声发射技术记录了预应力钢筋混凝土梁在3点弯曲荷载下整个破坏过程的声发射信号,并在研究了声发射累计能量随时间变化关系曲线的基础上,采用谱分析对声发射全波形数据进行了分析和处理。以全波形声发射信号的能量分布特性为依据,通过对时域和频域内信号的振幅、频率特征和构件破坏过程的比较,初步得出了预应力钢筋混凝土梁破坏过程的声发射信号的参数特性,发现全波形声发射信号能够实时反映预应力混凝土梁破坏过程中的特征信息。     

应用全差示光度法测定水和废水中的微量氰化物

在ｐＨ８０时,ＣＮ－与３甲基１苯基５吡唑啉酮、吡啶及氯胺Ｔ反应形成稳定的蓝色产物,最大吸收波长为６１７ｎｍ,表观摩尔吸收系数为１２×１０５Ｌ／ｍｏｌ·ｃｍ。实验了采用全差示光度法测定微量氰化物的条件。通过蒸馏富集,建立了改进的吡唑啉酮法测定氰化物的分析方法,并对实际样品进行了测定。本方法的检出限为０００１ｍｇＣＮ－／Ｌ,检测范围为０００１～０２５ｍｇＣＮ－／Ｌ,可应用于水和废水中微量氰化物的测定。     

聚类GO术语在基因表达差异研究中的应用

针对基因功能分类体系基因本体(Gene Ontology,GO)特殊的有向无环图特点,改进传统的用单个GO术语检测基因差异表达信号的缺陷,设计出"聚类GO术语提升差异表达检测(ScaGO)"算法.通过简单的输入对照和实验组表达谱上的全部基因表达信号,来研究一些比较新的差异表达功能组,有助于进一步解释基因差异表达的生物学意义,如疾病发病机制、药物作用机理等.将ScaGO和基于单GO术语差异分析法应用到急性淋巴细胞性白血病数据集和酵母Rap1 DNA绑定突变体差异表达数据集上,结果显示,ScaGO能比基于单GO术语差异分析法发现一些新的与差异表达相关联的功能类基因,对于指导实验具有积极意义.图1表3参21