好氧-厌氧两相堆肥过程中抗生素耐药基因的变化特征及影响因素研究

针对堆肥处理过程中抗生素耐药基因（antibiotic resistance genes,ARGs）去除效率不高、诱导潜在致病菌传播扩散等环境安全问题,采用好氧-厌氧两相堆肥处理猪粪、秸秆,探讨ARGs丰度及微生物群落的变化特征,并明确影响ARGs相对丰度变化的关键因子.结果表明:与单一好氧相比,好氧-厌氧两相堆肥能更有效地降低部分ARGs及潜在致病菌的相对丰度,其中ermB、tetK的相对丰度分别降低了39.68%、72.19%,假单胞菌属（Pseudomonas）、梭菌属（Clostridium_sensu_stricto_1）的相对丰度分别降低了96.09%、90.07%.主成分分析（Principal Component Analysis,PCA）及冗余分析（Redundancy analysis,RDA）得出,微生物群落和环境因子均显著影响ARGs相对丰度的变化（P < 0.05）,贡献率分别为82.0%、71.2%.其中,梭菌属（Clostridium_sense_stricto_1）、黄杆菌属（Flavobacterium）、尿素芽孢杆菌属（Ureibacillus）和海洋杆菌属（Oceanobacillus）是影响ARGs相对丰度变化的主要微生物菌属;温度、电导率、含水率和pH是影响ARGs相对丰度变化的关键环境因子,贡献率分别为22.0%、16.7%、16.2%和15.0%.研究显示:与好氧堆肥相比,好氧-厌氧两相堆肥能更有效地去除ARGs,并抑制潜在致病菌的繁殖扩散,从而减少环境风险;然而部分ARGs和潜在致病菌仍存在增殖现象,因此亟需进一步优化好氧-厌氧两相堆肥工艺.      

HYDROLOGIC DESIGN CONSIDERATIONS OF CONSTRUCTED WETLANDS FOR URBAN STORMWATER RUNOFF1

ABSTRACT: The successful design of constructed wetlands requires a continuous supply of water or vegetation that can withstand drought conditions. Having a constant water source is the best alternative to insure species diversity throughout the season. Consequently, detention structure designs should be based on times between events as well as on hydrologic return periods, since between events is when most evaporation and infiltration losses are likely to occur. In arid or semi-arid environments, this is a difficult process because of long interevent times and seasonal changes in precipitation patterns. This discussion is predicated on the assumption that phytoplankton, epiphytic algae, and emergent vegetation require moist conditions to be effective at removing nutrients, metals and other pollutants. There are drought tolerant species of vegetation that can be used in constructed wetlands but it may take several days to re-establish the attached bacteria communities necessary for optimum pollutant removal. This paper examines a stochastic framework to examine the probability of extended dry periods based on historic rainfall data. The number of consecutive dry days is selected for a specified level of assurance. By multiplying this value by the sum of daily system losses, an overall pond volume can be determined that ensures a minimum depth of water. To illustrate the utility of the approach, the method is applied to a site in Spokane, Washington.     

Temporal variations of CO2 concentration near land surface and its response to meteorological variables in Heihe River Basin, northwest China

Atmospheric CO2 concentration (CC) near land surface and meteorological variables have been measured at four sites,named Yeniugou (alpine meadow and permafrost), Xishui (mountainous forest), Linze (oasis edge) and Ejina (lower desert),respectively, in Heihe River Basin, northwest China. The results showed that, the half hourly CC at night was larger than in daytime,and the daily averaged CC was the largest in winter. The averaged CC of 932 d at the Linze was about 418 ppm, was about 366 ppm in the 762 d at the Ejina. In the same period from September 23 to November 9, 2004, the averaged CC was about 625,334, 436 and 353 ppm, at Yeniugou, Xishui, Linze and Ejina, respectively. The linear relationship between daily averaged CC and air temperature T was negative, between CC and relative humidity (RH) was positive. The linear CC-atmospheric pressure (A P) relationship was negative at the Linze and Yeniugou, was positive at the Ejina. The relationship between CC and global radiation R was exponent, and soil temperature Ts was negative linear, and soil water content was complex. The correlation between CC and wind speed was not existent.Using meteorological variables together to simulate CC, could give good results.     

单纤维过滤介质表面尘粒捕集的随机模拟

基于控制面概念和粒子运动轨迹计算,建立单纤维过滤介质表面尘粒捕集的三维随机计算模型,采用Ku-wabara流场表征单纤维表面的气流绕流特征。计算分析了St数、无量纲粒径及单纤维过滤模型填充密度对沉积物形态结构和积尘状态下单纤维捕集效率的影响。结果指出,St数对沉积物形态结构具有显著作用,随St数减小,沉积物由紧密的堆积结构演变为分叉显著的树枝状结构。模拟结果还发现,无论过滤条件如何变化,无量纲单纤维捕集效率随捕集粒子数呈近似线性增加关系。     

云南省耕地生产效率的时空差异及影响因素

以第二次全国土地调查完成以来的2009~2013年为研究时段,在测算云南省16个州(市)的耕地生产效率基础上,综合运用ESDA技术、基尼系数等方法系统分析云南省耕地生产效率的时空差异,并构建计量经济模型研究影响不同区域耕地生产效率变化的主要因素。结果表明:(1)2009~2013年,云南省耕地生产效率呈总体上升趋势,但增幅逐年降低;效率低值地区增长明显,高值地区则并不显著;(2)云南省耕地生产效率区域差异呈缩小态势,并表现出由西南向东北递减的空间分布特征;(3)滇中、滇西南等粮食主产区受≤6°耕地面积比重、旱涝保收面积的影响较大,而滇东地区则更易受到6°坡耕地面积和人均GDP的影响,滇西北地区受≤6°耕地面积比重和人均GDP的影响显著。     

How the redox state of tobacco 'Bel-W3' is modified in response to ozone and other environmental factors in a sub-tropical area?

This study intended to determine whether the redox state in plants of Nicotiana tabacum ‘Bel-W3’ fluctuates in response to the environmental factors in a sub-tropical area contaminated by ozone (São Paulo, SE - Brazil) and which environmental factors are related to this fluctuation, discussing their biomonitoring efficiency. We comparatively evaluated the indicators of redox state (ascorbic acid, glutathione, superoxide dismutase, ascorbate peroxidase, and glutathione reductase) and leaf injury in 17 field experiments performed in 2008. The redox state was explained by the combined effects of chronic levels of O₃ and meteorological variables 4-6 days prior to the plant sampling. Moderate leaf injury was observed in most cases. The redox state of tobacco decreases few days after their placement in the sub-tropical environment, causing them to become susceptible to oxidative stress imposed by chronic doses of O₃. Its bioindicator efficiency would not be diminished in such levels of atmospheric contamination.     

A stochastic approach for the operation of a wind and pumped storage plant under a deregulated environment

To improve the competiveness in the energy market, it is necessary that the wind power plants provide guaranteed power generation, although, it is not possible to forecast power availability from wind power plant accurately. This paper presents a stochastic model and solution technique for the combined operation of wind and pumped storage power plants to improve the power availability and increasing the profit considering uncertainties of wind power generation. In this model, uncertainties in wind data have been forecasted for grid connected day-ahead market using Weibull distribution model. The imbalances in the forecasted wind data and the market demand have been reduced by operating the pumped storage power plant. In this stochastic mixed integer problem, pumped storage plant can take the supply either from the grid or from the wind power plant for the pumping operation to store the energy in order to utilize this energy during peak hours for increasing the overall revenue. The reliability of the pumped storage is improved by replacing the conventional unit with the adjustable speed type pumped storage unit. In order to prove the optimality of the solution, two case studies were considered. In case study–I, scheduling is provided by operating the conventional pumped storage unit, whereas in case study–II, adjustable speed pumped storage unit has been used. It has been found that the adjustable speed pumped storage unit has further reduced the imbalance between generated power and demand. The complete approach has been formulated and implemented using AMPL software.     

Cyclo-stationary maximum cross-covariance analysis for exogenously forced environmental systems

The response of an ecological system to external forcings shows marked seasonality and complex time delays. In order to take seasonality into account, the assumption of stationarity inherent in most statistical models for time series is replaced here by more flexible cyclo-stationarity. To describe the dominant modes of the cross-covariance structure of the system and the forcing, temporal (orthonormal) patterns are derived. They are estimated by a singular value decomposition of the cyclo-stationary sample cross-covariance matrix. These patterns allow to formulate a prediction scheme for the system once the forcing is known. The method has comparatively little tendency for multi-collinearity, so that it is well applicable also for multivariate settings and shorter data records. The method is illustrated for an aquatic ecosystem with distinct phases of a biological succession.     

平稳地震地面运动改进金井清谱的相关函数模型

胡聿贤平稳地震地面运动谱模型是对Kanai-Tajimi模型的一种改进。通过数学推导证明胡聿贤谱是将基岩地震动模拟为过滤白谱过程,经具有某种特性的线性单自由度土层滤波后而形成的,从而在物理上解释了胡聿贤模型的合理性;研究了低频截止频率的作用,推导出胡聿贤模型的相关函数的表达式。本文所得结果可为结构随机地震反应时域分析提供基础。