Development and Evaluation of a Multiplexed Real-Time TaqMan RT-PCR Assay with a Sample Process Control for Detection of F-specific RNA Coliphage Genogroups I and IV

There are increasing concerns of zoonotic transmission of some animal enteric viruses, such as calicivirus, hepatitis E virus, and rotavirus, which are closely related to human pathogenic strains. Most enteric viruses are detected by molecular techniques because they cannot be cultured. Surrogates such as F-RNA coliphages are cultivable but few molecular methods exist. Individual real-time TaqMan RT-PCR assays for the replicase gene of F-RNA coliphage genogroups I and IV were developed and multiplexed with a real-time TaqMan RT-PCR assay for feline calicivirus as a sample process control for the simultaneous detection and enumeration of genogroup I and IV F-RNA coliphages. Genogroup IV were successfully detected with the multiplexed assay in 80% of fecal samples that contained F-RNA coliphage levels ≥3.2 log plaque forming units (pfu). F-RNA coliphage were at or below the limit of detection in most fecal samples when levels were ≤4 log pfu/g.     

辅助燃料对垃圾熔融炉炉温影响的分析研究

文章计算了在不同焦炭加入量及不同焦炭和煤的配比条件下处理城市生活垃圾时气化熔融炉的炉温。计算结果表明,用焚烧法处理重庆市的生活垃圾时,在常温鼓风条件下,要使垃圾灰渣完全熔融,焦炭配比至少应在42％以上;在一定的条件下,可以用煤部分或全部代替焦炭,同时配以高温鼓风,可使炉温达到并超过垃圾灰渣的熔融温度,从而使得垃圾灰渣熔融。     

从废水中回收沼气能及氢能与电能

厌氧发酵制沼气、厌氧发酵制氢和微生物燃料电池技术是利用微生物在废水处理的同时回收能源的三种主要方式。文章结合相关研究现状,介绍了这三种技术的基本原理及其在废水处理领域的发展状况和展望,并对三种生物能源进行了比较。     

生物阴极型微生物燃料电池同步降解偶氮染料与产电性能研究

构建了生物阴极型微生物燃料电池(BCMFC),研究了以葡萄糖-偶氮染料(活性艳红X-3B)为共基质条件下,BCMFC产电性能及偶氮染料的降解特性.结果表明,电能的产生源于BCMFC对葡萄糖的降解,共代谢下活性艳红X-3B的(ABRX3B)的生物降解是主要的脱色机理.当葡萄糖初始浓度为500mg·L-1(以COD计),ABRX3B浓度低于300 mg·L-1时,功率密度维持50.7 mW·m-2,最终脱色率在94.4%以上,而ABRX3B浓度的进一步提高对BCMFC产电会产生抑制作用.阳极液的COD去除率和UV-Vis光谱表明,ABRX3B的降解过程中,有中间产物的积累.共基质条件下,BCMFC可成功实现同步电能输出和高效脱色.     

Prospects for carbon-neutral housing: the influence of greater wood use on the carbon footprint of a single-family residence

This paper examines the energy and carbon balance of two residential house alternatives; a typical wood frame home using more conventional materials (brick cladding, vinyl windows, asphalt shingles, and fibreglass insulation) and a similar wood frame house that also maximizes wood use throughout (cedar shingles and siding, wood windows, and cellulose insulation) in place of the more typical materials used – a wood-intensive house. Carbon emission and fossil fuel consumption balances were established for the two homes based on the cumulative total of three subsystems: (1) forest harvesting and regeneration; (2) cradle-to-gate product manufacturing, construction, and replacement effects over a 100-year service life; and (3) end-of-life effects – landfilling with methane capture and combustion or recovery of biomass for energy production.The net carbon balance of the wood-intensive house showed a complete offset of the manufacturing emissions by the credit given to the system for forest re-growth. Including landfill methane emissions, the wood-intensive life cycle yielded 20 tons of CO₂e emissions compared to 72 tons for the typical house. The wood-intensive home's life cycle also consumed only 45% of the fossil fuels used in the typical house.Diverting wood materials from the landfill at the end of life improved the life cycle balances of both the typical and wood-intensive houses. The carbon balance of the wood-intensive house was 5.2 tons of CO₂e permanently removed from the atmosphere (a net carbon sink) as compared to 63.4 of total CO₂e emissions for the typical house. Substitution of wood fuel for natural gas and coal in electricity production led to a net energy balance of the wood-intensive house that was nearly neutral, 87.1 GJ energy use, 88% lower than the scenario in which the materials were landfilled.Allocating biomass generation and carbon sequestration in the forest on an economic basis as opposed to a mass basis significantly improves the life cycle balances of both houses. Employing an economic allocation method to the forest leads to 3–5 times greater carbon sequestration and fossil fuel substitution attributable to the house, which is doubled in forestry regimes that remove stumps and slash as fuel. Thus, wood use has the potential to create a significantly negative carbon footprint for a house up to the point of occupancy and even offset a portion of heating and cooling energy use and carbon emissions; the wood-intensive house is energy and carbon neutral for 34–68 years in Ottawa and has the potential to be a net carbon sink and energy producer in a more temperate climate like San Francisco.     

Application of response surface methodology for the optimization of biodiesel production from yellow mustard (Sinapis alba L.) seed oil

In the present study, response surface methodology (RSM) involving central composite design (CCD) was applied to optimize the reaction parameters of biodiesel production from yellow mustard (Sinapis alba L.) seed oil during the single-step transesterification process. A total of 30 experiments were designed and performed to determine under the effects of variables on the biodiesel yield such as methanol to oil molar ratio (2:1–10:1), catalyst concentration (0.2–1.0 wt.% NaOH), reaction temperature (50–70°C), and reaction time (30–90 min). The second order polynomial model was used to predict the biodiesel yield and coefficient of determination (R²) was found to be at 0.9818. The optimum biodiesel yield was calculated as 96.695% from the model with the following reaction conditions: 7.41:1 of methanol to oil molar ratio, 0.63 wt. % NaOH of catalyst concentration, 61.84°C of reaction temperature, and 62.12 min of reaction time. It is seen that the regression model results were in agreement with the experimental data. The results showed that RSM is a suitable statistical technique for optimizing the reaction parameters in the transesterification process in order to maximize the biodiesel yield.     

Dynamic contact angle effects on gas-liquid behaviors in the cathode of proton exchange membrane fuel cell with stirred tank reactor design

Liquid water management is still a critical issue in the improvement of proton exchange membrane fuel cell (PEMFC) performance. In this work, for the first time, the liquid water behavior and transport inside the cathode of a PEMFC with a stirred tank reactor (STR) design, rather than the conventional PEMFC flow channel design, are numerically studied. The dynamic contact angle (DCA) is applied to multiple wall boundaries in the numerical model through a user-defined-function (UDF) code, i.e., STR-DCA model. Another numerical model with the static contact angle (SCA) and same operating conditions, i.e., STR-SCA model, is also developed for comparison. The volume of fluid (VOF) method is employed in the simulation to track the gas-liquid interface. The results show that the liquid water distribution and transport are significantly different between these two models, indicating the remarkable effects of DCA on the simulation results. It is also verified the capability of STR-PEMFC to reduce the liquid water flooding, showing the potential of this channel-less type fuel cell in the further development.     

火电厂燃煤锅炉系统湿量平衡分析与计算

针对煤的有效热能降低、烟气管道和烟囱腐蚀严重,以及向环境中排放湿量过多等问题,对燃煤锅炉系统湿量来源进行分析,建立了锅炉燃烧系统湿量计算的基本模型。根据热力学基本原理和物料守恒原理,给出了燃煤锅炉系统湿量的计算方法。采用该方法对燃烧不同煤种锅炉的实际运行数据进行湿量计算分析比较。结果表明:通过理论分析计算得到的燃煤锅炉系统湿量与锅炉的实际运行时烟气湿量较吻合,所给出的燃煤锅炉系统湿量的计算方法可以用于锅炉燃烧系统的运行优化。     

有风条件下航空煤油池火燃烧特性的实验研究

在全尺寸热释放速率实验台的基础上,搭建有风条件池火实验平台,开展了不同风速条件下的航空煤油池火燃烧实验,实验所用正方形油盘的边长分别为0.2m、0.3m和0.4m,风速范围为0 m.s-1至4.99 m.s-1。实验结果表明,风速为0 m.s-1时航空煤油池火的燃烧速率随油盘尺寸增大而单调递增,实验值与理论值的差距随风速增大而减小。实验所得.m″windy/.ms″till与v/D呈线性关系,与前人结论一致,但实验所得参数与前人值不同。同尺寸油盘池火的热释放速率峰值来临时间随风速增大有减小的趋势;不同尺寸油池火的燃烧速率随风速增加而单调递增。对不同尺寸油池火的热释放速率峰值随风速的变化规律作了讨论。     

基于替代模型的地下水DNAPLs污染源反演识别

应用基于核极限学习机替代模型的模拟-优化理论和方法研究解决了地下水DNAPLs污染源及含水层参数的同步反演识别问题.结果表明：1）核极限学习机替代模型对模拟模型有较高的逼近精度,能够识别并模仿模拟模型的输入-输出关系,绝大部分相对误差小于5%,平均相对误差仅有2.98%;2）以替代模型代替模拟模型,大幅度地减小了模拟-优化过程的计算负荷,将反演识别时间由传统方法的83天减少到3小时,并能够保持较高的计算精度;3）应用基于模拟退火的粒子群优化算法求解优化模型,能够以较快的速度搜寻到全局最优,同时避免搜索过程陷于局部极小解.