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51.
CdSe/ZnS量子点对稀有鮈鲫胚胎发育的影响及其氧化应激作用 总被引:1,自引:0,他引:1
本研究以稀有鮈鲫(Gobiocypris rarus)为对象,研究了不同浓度CdSe/ZnS量子点(QDs)暴露下,稀有鮈鲫胚胎发育过程中自主运动频率、内心率和体长的变化,以及利用体内超氧化物歧化酶(SOD)和丙二醛(MDA)作为毒性指标,反映CdSe/ZnS QDs暴露对稀有鮈鲫胚胎发育的氧化应激作用.结果显示:CdSe/ZnS QDs对稀有鮈鲫胚胎72 hpf(hours post fertilization)的半致死浓度(LC50)为319.629 nmol·L-1,96 hpf的半致畸浓度(EC50)为203.312 nmol·L-1.CdSe/ZnS QDs暴露不仅影响稀有鮈鲫胚胎死亡率、畸形率、自主运动频率、孵化时间和孵化率,而且使其内心率减缓、体长缩短,导致胚胎卵凝结,心包囊肿,出现脊椎弯曲等多种毒性现象.同时发现,CdSe/ZnS QDs暴露导致稀有鮈鲫体内MDA含量增加以及SOD活力的降低.这表明CdSe/ZnS QDs对稀有鮈鲫胚胎发育具有致畸、致死作用,而氧化应激可能是引起其胚胎致畸、致死的重要机制之一. 相似文献
52.
2,2',4,4'-四溴联苯醚对赤子爱胜蚓的抗氧化酶、代谢酶及其基因表达的影响 总被引:6,自引:5,他引:1
多溴联苯醚(PBDEs)是广泛存在于环境中的一种新型持久性有机污染物.四溴联苯醚同分异构体中的BDE-47是多溴联苯醚中最重要的单体之一.试验采用人工土壤培养法,通过亚急性试验,研究了在不同暴露时间阶段下,不同BDE-47剂量对赤子爱胜蚓(Eisenia fetida)抗氧化酶(过氧化氢酶CAT)、代谢酶(谷胱甘肽转移酶GST)以及二者基因表达的影响.结果表明,在暴露14 d和28 d时,CAT活性被诱导上升并且差异显著;GST活性变化差异不显著;CAT基因表达水平在第14 d时呈现抑制效应,在后续的第28 d和第42 d基因表达水平上调;GST基因表达水平整体呈现诱导效应,并且差异显著,随着暴露时间的增加,低毒处理组(10、50 mg·kg-1)的基因表达水平逐渐下调至低于对照组的水平,高毒处理组(100、200 mg·kg-1)的基因表达量仍高于对照组水平;在BDE-47的暴露试验中,CAT与GST活性及其基因表达水平两项指标对低毒处理组较高毒处理组更为敏感. 相似文献
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黄连根茎和制剂具有抗菌等作用,广泛用于水产养殖,所造成水生态风险需要评估.试验设置总生物碱为0(CK),0.088(T1)、0.44(T2)和1.76 mg·L-1(T3)的黄连根茎浸提液(CRE)4种处理,研究了对斜生栅藻和蛋白核小球藻的毒理作用.结果表明,T1抑制绿藻生长,T2和T3使绿藻生长和繁殖停止;它们均显著降低绿藻叶绿素和蛋白质含量,说明CRE抑制光合作用和蛋白质合成是绿藻生长繁殖速率降低和死亡的直接原因.CRE使氢离子和胞内物质外流,导致藻液p H值显著降低和电导率提高.在T1和T2处理中,绿藻细胞SOD活性先升后降;在T3处理中,SOD活性显著降低.说明在CRE暴露初期,低中浓度的CRE诱导绿藻细胞产生抗性,随暴露时间增长或直接暴露在高浓度的CRE下,抗氧化酶系统被破坏.同样,随着CRE浓度增大,丙二醛含量增加,意味着绿藻细胞膜结构破坏,透性增加.CRE总体上对蛋白核小球藻的危害作用大于斜生栅藻.在水产养殖中,滥用黄连根茎或制剂,以及大规模集约化种植黄连对水体初级生产力具有潜在的生态风险. 相似文献
55.
在静态暴露的条件下,利用流式细胞术等检测技术,考察了不同浓度的溴酸盐对普通小球藻的生长以及生理特性的影响,并对溴酸盐的毒性作用机制进行了初步的探索.结果表明,当溴酸盐浓度达到8 mmol·L~(-1),持续暴露96 h时,能够引起普通小球藻比生长率显著降低、细胞膜完整性显著降低、酯酶活性显著升高、线粒体膜电位显著升高、活性氧水平显著增加.扫描电镜可直观表明细胞膜受到损伤.由此说明,由于溴酸盐毒性的持续作用,使得活性氧在藻细胞内积累,普通小球藻自身的调节功能不能及时消除过多的活性氧,从而引起细胞膜完整性、酯酶活性和线粒体膜电位出现异常情况,藻细胞生理功能出现紊乱,藻细胞结构遭到破坏,最终导致普通小球藻生长受到抑制或死亡. 相似文献
56.
电化学还原-氧化工艺降解4-氯酚的毒性研究 总被引:1,自引:1,他引:0
采用紫外光还原法制备了Pd-Fe/石墨烯催化阴极,并以Ti/IrO_2/RuO_2为阳极,构成三电极体系(双阴极)和两电极体系(单阴极)的电化学还原-氧化降解工艺,分别对4-氯酚进行降解.采用离子色谱、高效液相色谱、TOC仪对4-氯酚降解过程中中间产物及其浓度进行测定.根据公式计算降解过程中理论计算毒性值,应用发光细菌法测定降解过程中的实际毒性值,对理论计算毒性值与实际毒性值进行比较,分析不同体系下降解过程中毒性的变化规律.结果表明,两种工艺体系在最佳降解条件下,阴极室毒性均呈下降的趋势,由于降解过程中在阳极室生成高毒性的苯醌,阳极室毒性均先升高后降低.通过相关性分析得到,两种体系理论计算毒性与实际毒性在P=0.01水平下,相关性系数均为1,显著相关,表明降解过程中实际毒性的测定结果真实可靠.降解至120 min时,三电极体系毒性小于两电极体系,表明三电极体系优于两电极体系.据此提出实际毒性测定方法在电化学还原-氧化工艺降解氯酚类有机废水毒性测试的工业应用中有着广泛的前景. 相似文献
57.
锑的表生地球化学行为与环境危害效应 总被引:12,自引:0,他引:12
锑(Sb)是一种典型的有毒有害重金属元素。由于自然过程和人为活动的影响,锑及其化合物广泛分布于大气、土壤、水体等表生环境中,锑的环境污染日益严重。Sb不是植物必需元素,但能够被植物体及农作物吸收。Sb对人体和动物体产生慢性毒性及潜在致癌性。本文主要通过分析Sb的主要矿物、锑及其化合物在表生环境中的分布与迁移特性,来阐述人体可能的锑暴露途径及由此产生的环境危害效应。 相似文献
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59.
60.
Küster A Pohl K Altenburger R 《Environmental science and pollution research international》2007,14(6):377-383
Background, Goals and Scope During the last years the miniaturization of toxicity test systems for rapid and parallel measurements of large quantities
of samples has often been discussed. For unicellular algae as well as for aquatic macrophytes, fluorescence-based miniaturized
test systems have been introduced to analyze photosystem II (PSII) inhibitors. Nevertheless, high-throughput screening should
also guarantee the effect detection of a broad range of toxicants in order to ensure routinely applicable, high-throughput
measuring device experiments which can cover a broad range of toxicants and modes of action others than PSII inhibition. Thus,
the aim of this study was to establish a fast and reproducible measuring system for non-PSII inhibitors for aquatic macrophyte
species to overcome major limitations for use.
Methods A newly developed imaging pulse-amplitude-modulated chlorophyll fluorometer (I-PAM) was applied as an effect detector in short-term
bioassays with the aquatic macrophyte species Lemna minor. This multiwell-plate based measuring device enabled the incubation and measurement of up to 24 samples in parallel. The
chemicals paraquat-dichloride, alizarine and triclosan were chosen as representatives for the toxicant groups of non-PSII
herbicides, polycyclic aromatic hydrocarbons (PAHs) and pharmaceuticals and personal care products (PPCPs), which are often
detected in the aquatic environment. The I-PAM was used (i) to establish and validate the sensitivity of the test system to
the three non-PSII inhibitors, (ii) to compare the test systems with standardized and established biotests for aquatic macrophytes,
and (iii) to define necessary time scales in aquatic macrophyte testing. For validation of the fluorescence-based assay, the
standard growth test with L. minor (ISO/DIS 20079) was performed in parallel for each chemical.
Results The results revealed that fluorescence-based measurements with the I-PAM allow rapid and parallel analysis of large amounts
of aquatic macrophyte samples. The I-PAM enabled the recording of concentration-effect-curves with L. minor samples on a 24-well plate with single measurements. Fluorescence-based concentration-effect-curves could be detected for
all three chemicals after only 1 h of incubation. After 4–5 h incubation time, the maximum inhibition of fluorescence showed
an 80–100% effect for the chemicals tested. The EC50 after 24 h incubation were estimated to be 0.06 mg/L, 0.84 mg/L and 1.69
mg/L for paraquatdichloride, alizarine and triclosan, respectively.
Discussion The results obtained with the I-PAM after 24 h for the herbicide paraquat-dichloride and the polycyclic aromatic hydrocarbon
alizarine were in good accordance with median effective concentrations (EC50s) obtained by the standardized growth test for
L. minor after 7 d incubation (0.09 mg/L and 0.79 mg/L for paraquat-dichloride and alizarine, respectively). Those results were in
accordance with literature findings for the two chemicals. In contrast, fluorescence-based EC50 of the antimicrobial agent
triclosan proved to be two orders of magnitude greater when compared to the standard growth test with 7 d incubation time
(0.026 mg/L) as well as with literature findings.
Conclusion Typically, aquatic macrophyte testing is very time consuming and relies on laborious experimental set-ups. The I-PAM measuring
device enabled fast effect screening for the three chemicals tested. While established test systems for aquatic macrophytes
need incubation times of ≥ 7 d, the I-PAM can detect inhibitory effects much earlier (24 h), even if inhibition of chemicals
is not specifically associated with PSII. Thus, the fluorescence-based bioassay with the I-PAM offers a promising approach
for the miniaturization and high-throughput testing of chemicals with aquatic macrophytes. For the chemical triclosan, however,
the short-term effect prediction with the I-PAM has been shown to be less sensitive than with long-term bioassays, which might
be due to physicochemical substance properties such as lipophilicity.
Recommendations and Perspectives The results of this study show that the I-PAM represents a promising tool for decreasing the incubation times of aquatic macrophyte
toxicity testing to about 24 h as a supplement to existing test batteries. The applicability of this I-PAM bioassay on emergent
and submerged aquatic macrophyte species should be investigated in further studies. Regarding considerations that physicochemical
properties of the tested substances might play an important role in microplate bioassays, the I-PAM bioassay should either
be accompanied by evaluating physicochemical properties modeled from structural information prior to an experimental investigation,
or by intensified chemical analyses to identify and determine nominal concentrations of the toxicants tested. The chemicals
paraquat-dichloride, alizarine and triclosan were chosen as representatives for the toxicant groups of non-PSII herbicides,
PAHs and PPCPs which are often detected in the aquatic environment. Nevertheless, in order to ensure a routinely applicable
measuring device, experiments with a broader range of toxicants and samples of surface and/or waste waters are necessary.
ESS-Submission Editor: Dr. Markus Hecker (MHecker@Entrix.com) 相似文献