基于生态系统对宝鸡传统乡村旅游新模式探究

基于生态系统对宝鸡传统乡村旅游新模式进行了探究。首先通过分析宝鸡部分传统乡村旅游的现状,对宝鸡传统乡村旅游现状进行了介绍。提出宝鸡开展民俗旅游的一些不足之处;其次说明基于生态系统理念,对宝鸡传统乡村旅游和生态环境的促进作用;然后提出观光主题公园与乡村民俗旅游的结合,可以更好地促进乡村生态环境和乡村旅游的可持续性发展,更能充分开发旅游资源,丰富旅游项目,促进乡村环境和乡村经济的双重发展,使宝鸡传统乡村旅游新模式得到发展。     

Repeated batch and continuous degradation of chlorpyrifos by Pseudomonas putida

The present study was undertaken with the objective of studying repeated batch and continuous degradation of chlorpyrifos (O,O-diethyl O-3,5,6-trichloropyridin-2-yl phosphorothioate) using Ca-alginate immobilized cells of Pseudomonas putida isolated from an agricultural soil, and to study the genes and enzymes involved in degradation. The study was carried out to reduce the toxicity of chlorpyrifos by degrading it to less toxic metabolites. Long-term stability of pesticide degradation was studied during repeated batch degradation of chlorpyrifos, which was carried out over a period of 50 days. Immobilized cells were able to show 65% degradation of chlorpyrifos at the end of the 50th cycle with a cell leakage of 112 × 10³ cfu mL^?1. During continuous treatment, 100% degradation was observed at 100 mL h^?1 flow rate with 2% chlorpyrifos, and with 10% concentration of chlorpyrifos 98% and 80% degradation was recorded at 20 mL h^?1 and 100 mL h^?1 flow rate respectively. The products of degradation detected by liquid chromatography–mass spectrometry analysis were 3,5,6-trichloro-2-pyridinol and chlorpyrifos oxon. Plasmid curing experiments with ethidium bromide indicated that genes responsible for the degradation of chlorpyrifos are present on the chromosome and not on the plasmid. The results of Polymerase chain reaction indicate that a ~890-bp product expected for mpd gene was present in Ps. putida. Enzymatic degradation studies indicated that the enzymes involved in the degradation of chlorpyrifos are membrane-bound. The study indicates that immobilized cells of Ps. putida have the potential to be used in bioremediation of water contaminated with chlorpyrifos.     

Sustainable Development and Transition of Traditional Retailing Centers in Beijing from Consumers' Perspective

Based on the consumer questionnaires of the three traditional retailing centers,Qianmen,Wangfujing and Xidan in Beijing,this article analyzes the present features of consumer composition in the three retailing centers.What is more,the evolution course and function transition of traditional retailing centers is revealed based on the changing of consumer composition in different times.Lastly,the renewal method and development trends of traditional retailing centers are discussed based on the evaluation results in consumers' perspective.     

Rubber cultivation and sustainable development in Xishuangbanna,China

SUMMARY

Xishuangbanna in southwest China is a tropical and subtropical region with extraordinary traditional cultures and attractive landscapes. Rubber cultivation is a key production source undergoing rapid growth. It contributes substantially to improvement of the local economic situation and partially replaces traditional slash-and-burn agriculture in the uplands, but it also results in biodiversity loss and environmental degradation and disturbs the peaceful life of the indigenous people. We discuss the traditional ecological knowledge (TEK) preserved in daily practices and the present types of rubber plantations and suggest the application of TEK, in particular, the indigenous land-use experience to rubber cultivation, in order to promote sustainability in local economic development and ecological well-being.     

Traditional Plant Harvesting in Contemporary Fragmented and Urban Landscapes

Abstract: Ecosystem fragmentation and destruction can lead to restrictive administration policies on traditional harvesting by indigenous peoples from remaining ecosystem tracts. In New Zealand, concerns about endangered species and governmental policies that focus on species and ecosystem preservation have resulted in severely curtailed traditional harvesting rights. Although provision has been made for limited gathering of traditional plants from government‐administered conservation lands, it is unclear how much harvesting is undertaken on these lands and elsewhere and what this harvest might consist of. We interviewed seven expert Maori elders from the Waikato, New Zealand, to identify plant species they currently harvested and from where. We compared these data with the data we collected on permits issued for plant collecting on conservation lands in the same region. We sought to gain information on indigenous plant harvesting to determine the extent of permitted harvesting from conservation lands in the Waikato and to identify issues that might affect plant harvesting and management. Elders identified 58 species they harvest regularly or consider culturally important; over 50% of these species are harvested for medicinal use. Permit data from 1996 to 2006 indicated no apparent relationship between species of reported cultural significance and the number of permits issued for each of these species. Currently, few plant species are harvested from conservation lands, although some unofficial harvesting occurs. Elders instead reported that medicinal plants are frequently collected from urban and other public areas. They reported that plant species used for dyeing, carving, and weaving are difficult to access. Elders also discussed concerns such as spraying of roadsides, which resulted in the death of medicinal species, and use of commercial hybrids in urban planning. Local government may have an increasingly important role in supporting native traditions through urban planning, which takes account of cultural harvesting needs while potentially reducing future harvesting pressure on conservation lands. We suggest that active participation by the Māori community in the development and management of urban harvesting resources will result in positive outcomes.     

Indigenous Knowledge of Natural Resource Management and Population Control for Dong Ethnic Group in Guizhou Province

Dong ethnic people have rich indigenous knowledge in terms of their daily life and production, which plays an important role in the sustainable development of their village. This paper aims to understand traditional knowledge of Dong ethnic people in resource management and population control, including traditional resource management, traditional medicinal knowledge and village regulations in Zhanli Village in Southeast Guizhou Province. The research methods include key informant interview, group discussio...     

Effect of ultra-micro powder Dendrobium officinale on the molecular diversity of intestinal bacteria in mice with spleen-deficiency constipation北大核心CSCD

This research aimed to study the effect of ultra-micro powder Dendrobium officinale on the molecular diversity of intestinal bacteria in mice with spleen-deficiency constipation, in order to provide scientific basis for ultra-micro D. officinale therapy. The mice with spleen-deficiency constipation in treatment groups were respectively given the traditional decoction of D. officinale and 50% dose ultra-micro powder D. officinale. The metagenome DNA of intestinal flora was extracted from intestinal contents. Then ARDRA analysis was made after PCR by universal primer of bacteria. The results showed that OTUs, Shannon index and Brillouin index were the same between the normal group and the 50% dose ultra-micro powder group, and between the model group and the traditional decoction group, but greater in the former two groups than the latter ones. The similarity coefficient (0.500 0) was bigger between the 50% dose ultra-micro powder group and the normal group than between the model group and the traditional decoction group, which was 0.181 8. Both cluster analysis and principal components analysis showed that the molecular diversity of intestinal microbiotia in mice of 50% dose ultra-micro powder group was the closest to that in the normal group. The results showed that the weakened molecular diversity of intestinal bacteria in mice with spleen-deficiency constipation can be regulated by the two kinds of D. officinale treatments to different extent. The efficacy of 50% dose ultra-micro powder D. officinale is better than the traditional decoction.     

Digital PCR: a powerful new tool for noninvasive prenatal diagnosis?

Recent reports have indicated that digital PCR may be useful for the noninvasive detection of fetal aneuploidies by the analysis of cell-free DNA and RNA in maternal plasma or serum. In this review we provide an insight into the underlying technology and its previous application in the determination of the allelic frequencies of oncogenic alterations in cancer specimens. We also provide an indication of how this new technology may prove useful for the detection of fetal aneuploidies and single gene Mendelian disorders. Copyright © 2008 John Wiley & Sons, Ltd.