Analysis of bacterial community structures in two sewage treatment plants with different sludge properties and treatment performance by nested PCR-DGGE method

The bacterial community structures in two sewage treatment plants with different processes and performance were investigated by denaturing gradient gel electrophoresis （DGGE） of nested polymerase chain reaction （nested PCR） amplified 16S rRNA gene fragments with group-specific primers. Samples of raw sewage and treated effluents were amplified using the whole-cell PCR method, and the activated sludge samples were amplified using the extracted genomic DNA before the PCR products were loaded on the same DGGE gel for bacterial community analysis. Ammonia-oxidizing bacterial and actinomycetic community analysis were also carried out to investigate the relationship between specific population structures and system or sludge performance. The two plants demonstrated a similarity in bacterial community structures of raw sewage and activated sludge, but they had different effluent populations. Many dominant bacterial populations of raw sewage did not appear in the activated sludge samples, suggesting that the dominant bacterial populations in raw sewage might not play an important role during wastewater treatment. Although the two plants had different sludge properties in terms of settleability and foam forming ability, they demonstrated similar actinomycetic community structures. For activated sludge with bad settling performance, the treated water presented a similar DGGE pattern with that of activated sludge, indicating the nonselective washout of bacteria from the system. The plant with better ammonium removal efficiency showed higher ammonia-oxidizing bacteria species richness. Analysis of sequencing results showed that the major populations in raw sewage were uncultured bacterium, while in activated sludge the predominant populations were beta proteobacteria.     

Anaerobic biodegradation of benzene series compounds by mixed cultures based on optional electronic acceptors

A series of batch experiments were performed using mixed bacterial consortia to investigate biodegradation performance of benzene, toluene, ethylbenzene and three xylene isomers （BTEX） under nitrate, sulfate and ferric iron reducing conditions. The results showed that toluene, ethylbenzene, m-xylene and o-xylene could be degraded independently by the mixed cultures coupled to nitrate, sulfate and ferric iron reduction. Under ferric iron reducing conditions the biodegradation of benzene and p-xylene could be occurred only in the presence of other alkylbenzenes. Alkylbenzenes can serve as the primary subs＇rates to stimulate the transformation of benzene and p-xylene under anaerobic conditions. Benzene and p-xylene are more toxic than toluene and ethylbenzene, under the three terminal electron acceptors conditions, the degradation rates decreased with toluene 〉 ethylbenzene 〉 m-xylene 〉 o-xylene〉 benzene 〉 p- xylene. Nitrate was a more favorable electron acceptor compared to sulfate and ferric iron. The ratio between sulfate consumed and the loss of benzene, toluene, ethylbenzene, o-xylene, m-xylene, p-xylene was 4.44, 4.51, 4.42, 4.32, 4.37 and 4.23, respectively; the ratio between nitrate consumed and the loss of these substrates was 7.53, 6.24, 6.49, 7.28, 7.81, 7.61, respectively; the ratio between the consumption of ferric iron and the loss of toluene, ethylbenzene, o-xylene, m-xylene was 17.99, 18.04, 18.07, 17.97, respectively.     

Effect of copper on the degradation of pesticides cypermethrin and cyhalothrin

The influence of coexisting copper (Cu) ion on the degradation of pesticides pyrethroid cypermethrin and cyhalothrin in soil and photodegradation in water system were studied.Serial concentrations of the pesticides with the addition of copper ion were spiked in the soil and incubated for a regular period of time,the analysis of the extracts from the soil was carried out using gas chromatography (GC).The photodegradation of pyrethroids in water system was conducted under UV irradiation.The effect of Cu~(2 ) on the pesticides degradation was measured with half life (t_(0.5)) of degradation.It was found that a negative correlation between the degradation of the pyrethroid pesticides in soil and Cu addition was observed.But Cu~(2 ) could accelerate photodegradation of the pyrethroids in water.The t_(0.5) for cyhalothrin extended from 6.7 to 6.8 d while for cypermethrin extended from 8.1 to 10.9 d with the presence of copper ion in soil.As for photodegradation,t_(0.5) for cyhalothrin reduced from 173.3 to 115.5 rain and for cypermethrin from 115.5 to 99.0 min.The results suggested that copper influenced the degradation of the pesticides in soil by affecting the activity of microorganisms.However, it had catalyst tendency for photodegradation in water system.The difference for the degradation efficiency of pyrethroid isomers in soil was also observed.Copper could obviously accelerate the degradation of some special isomers.     

Effect of Sb dopant amount on the structure and electrocatalytic capability of Ti/Sb-SnO2 electrodes in the oxidation of 4-chlorophenol

Ti/Sb-SnO_2 anodes were prepared by thermal decomposition to examine the influence',of the amount of Sb dopant on the structure and electrocatalytic capability of the electrodes in the oxidation of 4-chlorophenol.The physicochemical properties of the Sb-SnO_2 coating were markedly influenced by different amounts of Sb dopant.The electrodes,which contained 5% Sb dopant in the coating, presented a much more homogenous surface and much smaller mud-cracks,compared with Ti/Sb-SnO_2 electrodes containing 10% or 15% Sb dopant,which exibited larger mud cracks and pores on the surface.However,the main microstructure remained unchanged with the addition of the Sb dopant.No new crystal phase was observed by X-ray diffraction(XRD).The electrochemical oxidation of 4-chlorophenol on the Ti/SnO_2 electrode with 5% Sb dopant was inclined to electrochemical combustion;while for those containing more Sb dopant,intermediate species were accumulated.The electrodes with 5% Sb dopant showed the highest efficiency in the bulk electrolysis of 4-chlorophenol at a current density of 20 mA/cm~2 for 180 min;and the removal rates of 4-chlorophenol and COD were 51.0% and 48.9%,respectively.     

具有超压/负压防护功能的急救车防生物污染的安全性试验研究

采用粘质沙雷菌气溶胶,对具有超压/负压防护功能的急救车防生物污染的安全性进行试验考核。分别测试气溶胶发生后30s,1min,5min,10min时,车厢在超压防护和负压防护下的过滤效率,其结果表明:急救车在超压防护状态下开启空调时的平均过滤效率能达到99.99%,不开空调时的平均过滤效率能达到99.95%;在负压防护状态下开启空调时的平均过滤效率能达到99.93%,不开空调时的平均过滤效率能达到99.95%。证明急救车能够通过生物污染区域安全运送、急救伤病员,也能够运送、急救生物污染伤病员或烈性传染病员确保沿途环境不受污染,该研究成果为应对生物恐怖袭击和突发公共卫生事件(烈性传染病)提供了一种安全可靠的机动医疗救治平台。     

火电厂对生态环境影响的总体评价

抛弃了以往在分析污染排放物对环境影响时只对不同污染物进行单独分析的做法,从单位污染物对大气的影响和生产单位有用能的污染物排放量两方面考虑,建立了一个通用的、能综合评价各种污染物对环境总体影响的模型--能源生态效率模型,并对燃烧不同燃料火电厂的能源生态效率作了计算,对能源生态效率的影响因素进行了分析.     

Anthropogenic metal cycles in China

The flows and stocks of seven important industrial metals were characterized for mainland China for several years in the dynamically changing decade of 1994–2004. One-year snapshot cycles are provided for chromium, nickel, and silver. For copper, zinc, lead, and iron, multiple-year cycles have been completed; they demonstrate that the flows of these metals into use in China doubled between 2000 and 2004. Although the Chinese per capita flows from production to disposal are mostly shown to be below the global average rate, they are increasing or are expected to increase dramatically. The metal resource efficiency is evaluated for several indicators of material flow analysis; these metrics for China are also below the global average values. The research quantitatively illustrates that China’s metal cycles may pose significant resource and environmental challenges in terms of their magnitudes and potential for growth.     

输变电工程建设的环保要求及其变化趋势分析

从环境敏感区的保护、公众参与、土地占用、施工期环境影响、景观协调性等方面对输变电工程规划、设计和建设过程中的环保要求以及变化趋势进行了较为全面的分析,对输变电工程规划、选址、选线、施工阶段等环节提出环境保护方面的意见和建议.     

山东半岛城市群城市就业结构变动特征分析

通过对山东半岛城市群就业结构的分析,揭示出山东省城市就业结构的一般规律。从对城市群各城市的分析来看,第一产业的就业人员占据较大比重,城市化水平较低。第二产业的从业人员比重较稳定,第三产业的就业比重总体上升,但其中的传统性行业的就业比重趋于下降而新兴行业比重提高。     

剩余污泥好氧堆肥生产有机复混肥的肥分及效益分析

城市污水处理厂剩余污泥含有大量有机质和氮、磷等营养元素,是农业生产宝贵的肥源。将污泥制成有机复混肥可以使作物增产,并改善土壤结构、提高土壤肥力。