Enhancement of UV and chromate mutagenesis by nickel ions in the Chinese hamster HGPRT Assay †

1. The HGPRT (Hypoxanthine‐Guanine‐Phospho‐Ribosyl‐Transferase) assay with Chinese Hamster V79 cells was used to measure the mutagenic effects of UV irradiation, potassium dichromate and nickel chloride. The agents were tested separately and in the combinations of UV plus nickel and dichromate plus nickel.

2. UV, Cr(VI) and Ni(II) were confirmed to be mutagenic in the V79 cell assay. The combination of UV(5J/m²) and Ni(II) (0.5 mM) caused a mutation rate 11.2 times above that corresponding to the sum of the individual mutation rates of these agents. The combined action of Cr(VI) (0.1 mM) and Ni(II) (0.5 mM) produced a mutation rate 2.8 fold above that corresponding to the sum of the individual rates of the separate agents.

3. The enhancing effect of nickel chloride on the mutagenicity of UV or Cr(VI) is interpreted by an interference of Ni(II) with the repair of DNA lesions.     

Disturbance regimes,resilience, and recovery of animal communities and habitats in lotic ecosystems

Disturbance regime is a critical organizing feature of stream communities and ecosystems. The position of a given reach in the river basin and the sediment type within that reach are two key determinants of the frequency and intensity of flow-induced disturbances. We distinguish between predictable and unpredictable events and suggest that predictable discharge events are not disturbances. We relate the dynamics of recovery from disturbance (i.e., resilience) to disturbance regime (i.e., the disturbance history of the site). The most frequently and predictably disturbed sites can be expected to demonstrate the highest resilience. Spatial scale is an important dimension of community structure, dynamics, and recovery from disturbance. We compare the effects on small patches (⩽1 m²) to the effects of large reaches at the river basin level. At small scales, sediment movements and scour are major factors affecting the distribution of populations of aquatic insects or algae. At larger scales, we must deal with channel formation, bank erosion, and interactions with the riparian zone that will affect all taxa and processes. Our understanding of stream ecosystem recovery rests on our grasp of the historical, spatial, and temporal background of contemporary disturbance events.     

Ecotoxicological effects assessment in the Netherlands: Recent developments

In a recently published annex to the National Environmental Policy Plan of the Netherlands (1989), attention was paid to ecotoxicological effects assessment. The proposed procedure was based on the advice of the Health Council of the Netherlands (1989) on risk assessment of toxic chemicals for ecosystems. The various extrapolation methods described by the Health Council are critically discussed in this paper. The extrapolation method of Van Straalen and Denneman (1989) is evaluated for eight chemicals and 11 aquatic species. Conclusions are drawn about the quality and quantity of the ecotoxicological data needed for aquatic effects assessment. For the soil—a compartment that is often at risk—ecotoxicological effects assessment is not possible because suitable ecotoxicological test methods still have to be developed.     

ECONOMIC EFFECTS OF RIVER RECREATION ON LOCAL ECONOMIES1

ABSTRACT: Outdoor recreation is a major, growing use of water resources in the United States. The economic effects of expenditures by visitors to three recreational river sites on local economies surrounding the sites were estimated using an input-output model (IMPL.AN). Expenditure data were from the Public Area Recreation Visitors Study (PARVS). Results indicate that visitor spending stimulates a considerable amount of economic activity and growth in local economies. Economic effects include increases in total gross output ranging from $2.6 million to $13.4 million, increases in total income ranging from $1.2 million to $5.6 million, and increases in employment ranging from 60 to 292 jobs.     

LONG-TERM PATTERNS OF WATER QUALITY IN A MANAGED WATERSHED IN OREGON: 1. SUSPENDED SEDIMENT1

The cumulative effects of forest management activities on water quality at a downstream point were monitored from 1972-1980 during development of a watershed for timber resources. Suspended sediment concentration and turbidity were measured at two hydrologic stations which bracketed a 10-km reach of the Middle Santiam River in the Western Cascades of Oregon as it flowed through an 8000-ha block of intensively managed forest land. Slope failures often accompany road building and harvesting in steep forested watersheds and pose the most serious threat to water quality. Although 180 km of road were constructed and 3400 ha of old-growth forests were harvested from slopes averaging over 60 percent, long-term changes in sediment yields remained undetectable during the period of measurement. The geologic characteristics of the basin and the road construction and maintenance techniques as prescribed by Oregon's forest practice regulations helped to minimize the occurrence of slope failures so that long-term changes in suspended sediment export rates did not occur. Throughout the nine-year measurement period, seven slope failures which added sediment directly to streams produced measurable short-term responses at the downstream sampling location, but these erosion events were too small and too infrequent to produce long-term changes in sediment yield from the watershed.     

FACTORS INFLUENCING ACUTE TOXICfrY OF COAL ASH TO RAINBOW TROUT (SALMO GAIRDNERI) AND BLUEGILL SUNFISH (LEPOMIS MACROCHIRUS)1

ABSTRACT: The potentially toxic components in coal ash (ash particles, heavy metals) were evaluated in laboratory static, acute (96 hr) bioassays, both separately and in various combinations with extreme pH (5.0 and 8.5), using rainbow trout (Salmo gairdneri) and bluegifi sunfish (Lepomis macrochirus). Ash particle morphology and metal distribution anlaysis, using electron microscopy and surface-subsurface analysis by ion microscopy, showed that metals could be either clumped or evenly distributed on the surface of fly ash. Surface enrichment on fly ash particles from electrostatic precipitators, as measured by ion microscopy, was found for cadmium, copper, chromium, nickel, lead, mercury, titanium, arsenic, and selenium. Bottom (heavy) ash was not acutely toxic to either fish species at concentrations of up to 1500 mg/l total suspended solids (TSS) at pH 5.0, 7.5, or 8.5. Fly ash particles were not acutely toxic to blue-gill at levels up to 1360 mg/l TSS. Rainbow trout were highly sensitive to fly ash (25 to 60 percent mortality) at concentrations of 4.3 to 20.5 mg/I TSS when dissolved metal availability was high but were not sensitive at higher particulate concentrations (58 to 638 mg/I TSS) when dissolved metals were low. When metals were acid-leached from fly ash prior to testing, no rainbow trout mortality occurred at TSS concentrations of up to 2,350 mg/l TSS. When the percent of dissolved metal was high (e.g., 50–90 percent of the total), fish mortality was increased. Rainbow trout were nearly two orders of magnitude more sensitive than bluegill when subjected to a blend of cadmium, chromium, copper, nickel, lead, and zinc. The two species were similar in their acute sensitivity to acidic pH at levels at or below 4.0 and alkaline pH of 9.1. If the pH of coal ash effluent is contained within the range 6.0 to 9.0, acute toxicity to fish can be attributed to trace element availability from fly ash but not heavy ash. Control of holding pond and effluent pH and maximizing pond residence time are important strategies for minimizing effects of ash pond discharges on fish.     

EFFECTS OF RATE STRUCTURE KNOWLEDGE ON HOUSEHOLD WATER USE1

ABSTRACT: Socioeconomic determinants of individual household water use were estimated using regressions of these characteristics with actual household water use for winter and summer. Results were disaggregated between those consumers who were aware of an increasing block rate price structure and those who were not. Most of the informed group members believed that this price structure did result in significant reductions in water use. Nevertheless, overall water use was greater for the informed group. The determinants of water use were found to differ between informed and uninformed users as well as between winter and summer. The uninformed users were influenced by a larger set of variables in each season than the informed group. In winter, the informed group members with swimming pools and/or arid landscaping used less water than their uninformed counterparts. Summer water use increased with length of tenancy in home for the uninformed group but not for informed, while increasing with ownership for informed consumers.     

The case for free market environmentalism

Environmental Ethics is the ethics of how we humans are to relate to each other about the environment we live in. The best way to adjust inevitable differences among us in this respect is by private property. Each person takes the best care of what he owns, and ownership entails the free market, which enables people to make mutually advantageous trades with those who might use it even better. Public regulation, by contrast, becomes management in the interests of the regulators, or of special interests, such as lovers of rare species-not the people they're supposed to be serving.     

Community opinion concerning airport noise-abatement alternatives

In the present study we examined (1) community opinion of airport noise-abating alternatives and (2) the influence of perceived control on attitudes concerning noise-abatement policies and annoyance toward aircraft noise. We proposed that residents who perceive themselves as having little control over aircraft noise exposure would express higher levels of annoyance than residents who believe they have some degree of control. Furthermore, residents with low levels of perceived control would be less favorable toward proposed noise-abatement alternatives. Survey results collected from 239 households within the 65 CNEL contour of a community airport in Southern California support these hypotheses. Findings indicate strong public support for noise abatement and strong opposition to land-use alternatives. In addition, residents' perceptions of control in the airport planning and management process are significantly related to their annoyance with noise and their opinions about noise management policies.