Acoustic orientation in the palmate newt, Lissotriton helveticus

Experiments reported here were carried out to investigate the use of acoustic cues by palmate newts (Lissotriton helveticus) for orientation and to study whether this behavior is learned, or whether two populations of palmate newts that cohabit with different frog species (Iberian green frog, Rana perezi, and European common brown frog, Rana temporaria) show different phonotactic preferences. The orientation tests consisted of presenting a control stimulus (white noise), a sympatric acoustic stimulus (calls of R. perezi or R. temporaria, depending on the origin of newts), or an allopatric stimulus (calls of natterjack toads, Bufo calamita, or R. perezi). Newts were released in a circular arena, while the acoustic stimuli were presented outside of the circular arena in four different compass orientation directions (0, 90, 180 and 270°). In this study, we show that L. helveticus performed positive phonotaxis toward the calls of R. perezi only when both species shared habitat, orienting randomly when R. perezi was absent from the newt’s natal population. Newts from both populations oriented randomly when exposed to the allopatric and control acoustic stimuli. These results suggest, for the first time, that recognition of the sympatric heterospecific calls could be learned. However, newts sharing the breeding pond with a population of R. temporaria oriented randomly when exposed to the calls of this species. The fact that the breeding seasons of R. temporaria and L. helveticus do not overlap in time does not allow the use of R. temporaria calls as a guidance mechanism for migrating individuals of L. helveticus.     

Magnetic compass mediates nocturnal homing by the alpine newt, Triturus alpestris

Experiments were carried out to investigate the use of magnetic compass cues in the nocturnal homing orientation of the alpine newt Triturus alpestris. Tests were carried out at a site 9 km to the east–northeast of the breeding pond. Newts were tested at night in an outdoor circular arena that provided an unimpeded view of celestial cues, in one of four symmetrical alignments of an earth-strength magnetic field. In tests carried out under partly cloudy skies newts exhibited homeward magnetic compass orientation. Because the moon was visible in some trials, but obscured by clouds in others, we investigated whether the presence of the moon contributed to the scatter in the distribution of magnetic bearings. When the moon was visible, the distribution of magnetic bearings was more scattered than when the moon was obscured by clouds, although in neither case was the distribution significant due, in part, to the small sample sizes. Moreover, when the moon was visible, newts oriented along a bimodal axis perpendicular to the moon azimuth, suggesting that the presence of the moon may have affected the newts behavior. To provide a more rigorous test of the role of magnetic compass cues when celestial cues were unavailable, nocturnal tests were carried out during the following migratory season under total overcast. In the absence of celestial compass cues, the distribution of magnetic bearings exhibited highly significant orientation in the homeward direction. These findings indicate that newts are able to orient in the homeward direction at night using the magnetic compass as the sole source of directional information. Moon light altered the newts behavior. However, this apparently resulted from the asymmetrical distribution of moon light in the testing arena, rather than the use of an alternative compass.     

Effect of water temperature on the courtship behavior of the Alpine newt Triturus alpestris

Temperature is expected to have an effect on the behavioral patterns of all organisms, especially ectotherms. However, although several studies focused on the effect of temperature on acoustic displays in both insects and anurans, almost nothing is known about how environmental temperature may affect ectotherm visual courtship displays and sexual performance. The purpose of this study was to determine the effect of environmental temperature on the sexual behavior of Alpine newts (Triturus alpestris). We subjected T. alpestris to two different temperatures in controlled laboratory conditions. Temperature had a major effect on both male and female behaviors: at low temperature, the frequencies of several displays, including tail-raising during sperm deposition, are lowered. This variation is caused indirectly by temperature because it is due to female responsiveness, which is temperature-dependent. However, the fanning movement of the males tail during its main courtship display is independent of female behavior: at lower temperatures, the tail beats at a lower rate, but for a longer time. The similar reproductive success (i.e. sperm transfer) at the two temperature ranges indicates that breeding in cold water is not costly but instead allows males and females to mate early in the season. This is particularly adaptive because, in many habitats, the reproductive period is shortened by drying or freezing conditions, which may impair survival of branchiate offspring. This study also demonstrates the necessity of considering environmental parameters when modeling optimality and characteristics of ectotherm behaviors.Communicated by W. Cooper