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Sung-Geun Woo Holly L. Sewell Craig S. Criddle 《Frontiers of Environmental Science & Engineering》2022,16(10):127
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斑马鱼基因组随机扩增DNA多态性研究 总被引:2,自引:0,他引:2
采用随机扩增DNA多态性(RAPD)技术,对毒性试验的模式生物斑马鱼(Danio rerio)基因组进行了分析。结果显示,在Amersham Pharmacia公司随机扩增引物系列中,分别在01号引物的829bp、534bp,02号引物的572bp,及06号引物的495pb,各出现1条稳定条带,而环磷酰胺作用后的斑马鱼基因组DAN随机扩增产物中,缺少了部分稳定条带。这可以明确判定受试斑马鱼的基因发生了改变,为水环境遗传毒性效尖的研究提供了初步的数据基础。 相似文献
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Ayşe N. Kiraççakali 《Chemistry and Ecology》2020,36(7):651-662
ABSTRACT Deltamethrin is a widely used pyrethroits worldwide. Although the chemical is used to combat insects, it has effects on other non-target organisms. Deltamethrin is extensively used in agriculture, animal husbandry, and domestic areas in the Lake Van basin. The aim of this study was to determine the cytotoxic, genotoxic, and oxidative damage from deltamethrin on the primary hepatocyte culture of Lake Van fish (Alburnus tarichi). In this study, the toxic effects of different concentrations (0.01, 0.1, 1, and 10?µM) of deltamethrin in the primary hepatocyte culture of Lake Van fish were investigated via liver enzymes aspartase aminotransferase (AST) and alanine aminotransferase (ALT), and the total antioxidant status (TAS), total oxidant status (TOS), lipid peroxidation (MDA), and DNA damage (8-OHdG). Deltamethrin caused an increase in the AST and ALT levels dependent on the dosage and time. The TAS and TOS levels increased at the end of 24?h and there was no difference at the end of 48?h. Deltamethrin did not affect the MDA level, but increased the 8-OHdG (P?<?0.05). In conclusion, it can be said that high doses of deltamethrin (1 and 10?µM) have a toxic effect on the primary hepatocyte culture of Lake Van fish. 相似文献
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Pheromones produce dramatic behavioral and physiological responses in a wide variety of species. Releaser pheromones elicit
rapid responses within seconds or minutes, while primer pheromones produce long-term changes which may take days to manifest.
Honeybee queen mandibular pheromone (QMP) elicits multiple distinct behavioral and physiological responses in worker bees,
as both a releaser and primer, and thus produces responses on vastly different time scales. In this study, we demonstrate
that releaser and primer responses to QMP can be uncoupled. First, treatment with the juvenile hormone analog methoprene leaves
a releaser response (attraction to QMP) intact, but modulates QMP’s primer effects on sucrose responsiveness. Secondly, two
components of QMP (9-ODA and 9-HDA) do not elicit a releaser response (attraction) but are as effective as QMP at modulating
a primer response, downregulation of foraging-related brain gene expression. These results suggest that different responses
to a single pheromone may be produced via distinct pathways. 相似文献
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