Genotoxicity of chromium in vitro on yeast: Interaction with DNA†

The genotoxicity of chromium chloride was investigated in cells of D₇ strain of Saccharomyces cerevisiae harvested either from logarithmic or stationary growth phase.

A weak induction of mifotic gene conversion and point reverse mutation was obtained when the incubations were performed using phosphate buffer. No genetic effect was observed when the incubations were performed using Tris‐HCl buffer.

The experiments with ⁵¹Cr radiotracer demonstrated that Cr³⁺ ion enters the yeast cells and binds to DNA even if the incubation mixture was performed with Tris HCl buffer. This behaviour could be due to the highest concentration of CrCl₃ that cause some damages to cytoplasmatic membrane.     

Molecular composition of recycled organic wastes,as determined by solid-state 13C NMR and elemental analyses

Using solid state ¹³C NMR data and elemental composition in a molecular mixing model, we estimated the molecular components of the organic matter in 16 recycled organic (RO) wastes representative of the major materials generated in the Sydney basin area. Close correspondence was found between the measured NMR signal intensities and those predicted by the model for all RO wastes except for poultry manure char. Molecular nature of the organic matter differed widely between the RO wastes. As a proportion of organic C, carbohydrate C ranged from 0.07 to 0.63, protein C from <0.01 to 0.66, lignin C from <0.01 to 0.31, aliphatic C from 0.09 to 0.73, carbonyl C from 0.02 to 0.23, and char C from 0 to 0.45. This method is considered preferable to techniques involving imprecise extraction methods for RO wastes. Molecular composition data has great potential as a predictor of RO waste soil carbon and nutrient outcomes.     

Interannual Changes in the C:N:P Ratios of Seston and Zooplankton of a High Mountain Lake in Sierra Nevada,Spain

We investigated the carbon, nitrogen and phosphorus content inthe seston and the zooplankton of a high-mountain lake duringthree years of contrasting physical and chemical conditions.Carbon in seston was ten times higher in 1995 than in 1996 and1997. Phosphorus content in seston was variable for the studyperiod, increasing gradually towards late summer in 1995, butpeaking abruptly in 1996 and 1997, reflecting atmosphericdepositions. Seston C:P were high and did not fit any definitepattern in 1995, and were particularly low after the thaw, increasing towards mid-summer in 1996 and 1997. As the seasonprogressed, major decreases in these ratios occurred coincidingwith important atmospheric inputs in the lake area. ZooplanktonN:P and C:P were negatively associated to the appearance ofrotifers and copepod nauplii in 1995, but positively correlatedto the ontogenetic development of the most abundant species,Mixodiaptomus laciniatus, in 1996. Seasonal variations inzooplankton N:P and C:P ratios showed limited interannual, butlarge intraannual variability. The comparison between the bulkcarbon in seston and zooplankton demands for this elementindicated that zooplankton were above food-quantity thresholdsfor maximum growth in 1995, 1996 and mid-season of 1997,therefore suffering only from food-quantity constraints afterthe thaw (nauplii dominance) and towards late season (adultdominance) in the latter year. The high C:P ratios in sestonrelative to zooplankton in 1995 imply that the zooplankton mayhave faced severe food-quality constraints (in terms ofphosphorus) during this year. Differences between bulkzooplankton and seston elemental nutrients are also discussed inrelation to the competitive abilities of species, andparticularly of Daphnia.     

洗涤剂中的磷对环境的影响及对策的探讨

本文介绍了关于洗涤剂中磷对环境影响的两种典型观点和解决的办法,并从经济和环境的角度进行全面的分析,得出合理的结论和建议。     

Monitoring of eutrophication and nutrient limitation in the Izmir Bay (Turkey) before and after Wastewater Treatment Plant

The distribution of inorganic nutrients and phytoplankton chlorophyll-a was investigated and N/P ratios were determined in Izmir Bay during 1996-2001. The average concentrations showed ranges of 0.01-0.19 and 0.01-10 microM for phosphate-phosphorus; 0.11-1.8 and 0.13-27 microM for (nitrate+nitrite)-nitrogen, 0.30-4.1 and 0.50-39 microM for silicate and 0.02-4.3 and 0.10-26 microg l(-1) for chlorophyll-a in the outer and middle-inner bays, respectively. The results are compared with the values obtained from the relatively unpolluted waters of the Aegean Sea. The N/P ratio is significantly lower than the assimilatory optimal (N/P=15:1) in conformity with Redfield's ratio N/P=16:1. Nitrogen is the limiting element in the Izmir Bay. Phosphate, which originates from detergents, is an important source for eutrophication in the bay, especially in the inner bay. In early 2000, a Wastewater Treatment Plant (WTP) began to treat domestic and industrial wastes. This plant treats the wastes about 60% capacity between 2000 and 2001. The sampling periods cover before and after treatment plant. Although the capacity of wastewater plant is sufficient for removal of nitrogen from the wastes, it is inadequate for removal of phosphate. This is also in accordance with the decreasing N/P ratios observed during 2000-2001 (after WTP) in the middle-inner bays.     

Effects of Poly(Ethylene Glycol) on the Production of Poly(β-Hydroxybutyrate) by Azotobacter vinelandii UWD

Azotobacter vinelandii UWD, ATCC 53799, an engineered strain derived from Azotobacter vinelandii UW was used in the poly(ethylene glycol) (PEG)-modulated synthesis of poly(-hydroxybutyrate) (PHB). To the best of our knowledge, this is the first report on modulating the production of PHB by amending the fermentation broth with PEG using A. vinelandii UWD. It was determined that A. vinelandii UWD is prone to back-mutation to the parent strain; hence fermentation experiments require the use of the antibiotic rifampicin. Diethylene glycol (DEG) and PEGs with molecular weights of 400, 2000, and 3400 Da and pentaerythritol ethoxylate (PEE) were used in the modulated fermentation experiments in a concentration of 2% (w/v). The molecular weight of the resulting polymers was reduced by up to 78%. No impact on the productivity of the strain was observed. Spectroscopic evidence showed that PEG-modulated synthesis resulted in the covalent attachment of the ethylene glycol moiety only when a small molecule, DEG, was used. PEGs had the same effects on the polymer formation in terms of molecular weight reduction as DEG, but no spectroscopic evidence was found for the formation of a covalent linkage between PHB and higher molecular weight PEGs.     

对铁碳处理硝基酚废水的研究

铁碳降解水中难降解有机污染物的影响因素、最佳工艺参数及处理效果;初步探讨了氧化降解污染物的作用机理;通过分析污染物降解的中间产物,提出了污染物降解的可能途径.探讨了pH、铁碳用量、温度以及锰矿物的粒径等对处理效果的影响,在最佳的工艺条件下,CODCr的去除率达到95%以上,TOC测定表明：大部分硝基酚被氧化降解为H2O和CO2.对硝基酚的降解途径主要是微电解将对硝基酚还原为对氨基酚,对氨基酚在酸性条件下被软锰矿氧化为H2O和CO2做探索性研究.     

设施农业土壤磷素累积迁移转化及影响因素

为了科学合理施用磷肥,减小对设施农业环境带来的污染风险,以北京市大兴区设施农业集中区为研究对象,通过对不同种植年限设施农业剖面土壤(0～100 cm)磷素含量的测定分析,探究磷素累积与迁移转化特征.结果表明,设施农业表层土壤全磷和有效磷含量变化范围大,显著高于周边粮田土壤,主要跟不同种植年限农户的施磷量相关,随着土层深度的增加,全磷和有效磷含量逐渐减小,呈现表聚特征,其中土壤ω(全磷)范围在0.38～2.58 g·kg^-1,ω(有效磷)范围在1.60～256.00 mg·kg^-1.随着种植年限的增加,土壤全磷和有效磷含量呈现先增加后减小的趋势,在15 a左右达到峰值,随后逐渐减少,趋于稳定,总体处于较高水平.无机磷主要集中在设施农业表层土壤,其中Ca-P占无机磷的比例最大,达到了98.38%,Ca₁₀-P是最主要的Ca-P形态,含量占Ca-P的78.70%,Ca₂-P占比最小,仅占9.50%.不同形态无机磷含量呈现表层土壤富集,向下减少的垂直分布特征;不同土层深度,不同形态无机磷占全磷比例变化存在差...     

虹鳟鱼鳃及肝脏多种CYP1基因表达模式作为生物标志物监测海河水污染状况

以虹鳟鱼为受试生物,以鳃与肝脏多种CYP1基因为生物标志物,在天津境内开展了野外监测实验,分析多种CYP1转录物的表达量变化及它们在不同监测位点的表达模式.实验通过鱼类吊网在特定水域进行野外监测.结果表明,葛沽与海河入海口的6种CYP1基因表达模式很相近,但表达量不同,表明两地的污染成分相近似但水中污染物负荷不同.除了在马厂减河外,CYP1C1/C3在葛沽与入海口受到明显诱导作用,表明马厂减河中存在的污染物种类不同于其它监测位点.因此,利用虹鳟鱼鳃与肝脏中多种CYP1基因表达模式作为生物标志物监测污染物有着很好的前景,不仅可以作为研究环境污染物对水环境和鱼类健康影响的重要手段,还可以为进一步解释化学混合物成分与多种生物反应之间关系提供理论基础.