Biofiltration of trimethylamine, dimethylamine, and methylamine by immobilized Paracoccus sp. CP2 and Arthrobacter sp. CP1

A biofilter using granular activated carbon with immobilized Paracoccus sp. CP2 was applied to the elimination of 10–250 ppm of trimethylamine (TMA), dimethylamine (DMA), and methylamine (MA). The results indicated that the system effectively treated MA (>93%), DMA (>90%), and TMA (>85%) under high loading conditions, and the maximum degradation rates were 1.4, 1.2, and 0.9 g-N kg⁻¹ GAC d⁻¹. Among the three different amines treated, TMA was the most difficult to degrade and resulted in ammonia accumulation. Further study on TMA removal showed that the optimal pH was near neutral (6.0–8.0). The supply of high glucose (>0.1%) inhibited TMA removal, maybe due to substrate competition. However, complete TMA degradation was achieved under the co-immobilization of Paracoccus sp. CP2 and Arthrobacter sp. CP1 (96%). Metabolite analysis results demonstrated that the metabolite concentrations decreased by a relatively small 27% while the metabolite apparently increased by heterotrophic nitrification of Arthrobacter sp. CP1 in the co-immobilization biofilter.     

Effects of cultivation conditions on the diversity of microbes involved in the conversion of rice straw to fodder

To confirm the optimum cultivation conditions for analyzing lactic acid bacterial communities and to provide the cultivation foundation for lactic acid bacterial communities that were used to convert straw into fodder, fermented rice straw was inoculated into 13 different broths. After 48 h of cultivation, pH values, volatile products, and microbial diversity were analyzed. Except for LAB broth, the pH values of the other broths could decrease to approximately 4.5. GC/MS analysis showed that lactic acid in Tomato MRS broth, MRS broth, LAB broth, and Tomato juice broth was higher than that in the other broths. DNA concentration analysis showed that the counts of microbes in Tomato MRS broth were 2.5 times of those in other broths and that tomato juice favored the reproduction of the microbes. Denaturing gradient gel electrophoresis （DGGE） analysis showed that the number of lactic acid bacterial species in HYA broth, Tomato juice broth, and Tomato MRS broth were higher than those in the other broths.     

Isolation and preliminary characterization of a 3-chlorobenzoate degrading bacteria

A study was conducted to compare the diversity of 2-, 3-, and 4-chlorobenzoate degraders in two pristine soils and one contaminated sewage sludge. These samples contained strikingly different populations of mono-chlorobenzoate degraders. Although fewer cultures were isolated in the uncontaminated soils than contaminated one, the ability of microbial populations to mineralize chlorobenzoate was widespread. The 3- and 4-chlorobenzoate degraders were more diverse than the 2-chlorobenzoate degraders. One of the strains isolated from the sewage sludge was obtained. Based on its phenotype, chemotaxonomic properties and 16S rRNA gene, the organism S-7 was classified as Rhodococcus erythropolis. The strain can grow at temperature from 4 to 37℃. It can utilize several （halo）aromatic compounds. Moreover, strain S-7 can grow and use 3-chlorobenzoate as sole carbon source in a temperatures range of 10-30℃ with stoichiometric release of chloride ions. The psychrotolerant ability was significant for bioremediation in low temperature regions. Catechol and chlorocatechol 1,2-dioxygenase activities were present in cell free extracts of the strain, but no （chloro）catechol 2,3- dioxygenase activities was detected. Spectral conversion assays with extracts from R. erythropolis S-7 showed accumulation of a compound with a similar UV spectrum as chloro-cis,cis-muconate from 3-chlorobenzoate. On the basis of these results, we proposed that S-7 degraded 3-chlorobenzoate through the modified ortho-cleave pathway.     

基于地块尺度的耕地非农化驱动力空间异质性研究——以武汉市为例

土地用途管制和基本农田保护制度下,耕地非农化概率值和其驱动力的空间作用强度分布在土地资源配置和耕地保护方面有积极的指示性。基于武汉市2000~2011年耕地非农化地块的微观数据,通过比较分析Logistic回归模型和地理加权Logistic回归模型参数估计结果,验证了耕地非农化驱动力的空间异质性,并对异质性的空间分布规律和政策涵义做出总结分析。研究表明:1耕地间的空间依赖效应和建设用地对耕地的空间溢出效应在耕地非农化过程中具有显著影响力,且耕地非农化驱动力存在显著的空间异质性;2地理加权Logistic回归模型由于考虑到数据的空间非平稳状态所以比Logistic回归模型有更好的拟合效果;3耕地非农化驱动力的异质性在空间上呈现出一定规律,这些受到产业布局、经济和城市发展特点等的影响,根据不同驱动力空间异质性产生的原因进行差异化土地管理可以解决耕地保护和建设用地供给间的矛盾。研究结果能从地块尺度上反映出耕地非农化驱动因素的空间异质性,实现了驱动因素作用强度空间分布的可视化,并能为国土资源差异化管理提供理论和实践参考。     

基于外部性内化的农地城市流转调控政策工具研究进展评述

本研究采用文献回顾法、归纳法,从农地城市流转外部性的分析与测度及政策工具在农地城市流转调控中的运用两个方面,对国内外相关研究进行了归纳与总结。结果表明,现有研究从内容和方法上都有待进一步的丰富和深入:在农地城市流转外部性的理论分析和定量测算方面,其外部性的内涵、分类与边界等尚未得到严格的界定与划分,评估技术的选择和应用尚存亟待回答的问题;在基于外部性内化的农地政策流转调控政策工具的运用方面,尚需在分析思路、政策工具的系统化和细节设计方面有所深入;在实证研究方面,亟需结合我国国情结合具体事例探讨政策工具运用的预期效果及不同政策工具可能的交互作用;此外,应考虑不确定与信息不对称对调控政策工具运行效率的影响。因此,我国学者应借鉴国内外已有的研究成果和经验,从外部性内化的角度设计适于我国国情的政策工具,从而为农地城市流转调控提供可靠的理论依据,以达到合理规制农地转用秩序的目的。     

4种实用配方饲料对锦鲤诱食活性和生长的影响

试验用鱼选择锦鲤.根据动物摄食行为学原理,采用迷宫法和摄食率法为试验方法,配制4种添加甘氨酸的实用饵料,研究其对锦鲤诱食活性和生长的影响,实验结果用Excel和SPSS17.0软件进行分析.结果表明,4种实用饵料都对锦鲤具有诱食反应.其中,火棘(35m甘氨酸)P=0.003＜ 0.05,浒苔(35mg甘氨酸)P=0.001＜0.05,诱食效果有极显著意义.与对照组相比,喂食实用饵料的锦鲤增重率都有所提高,其中浒苔+50mg甘氨酸最为明显,达到8.44％,饵料系数降低到4.123.     

农地非农化经济驱动机制的理论分析与实证研究

协调好经济发展和农地保护的矛盾是实现我国社会经济可持续发展战略的重要基础,而农地非农化又是这一矛盾的焦点,合理调控农地非农化需要掌握其驱动因素的作用机理,辨识主导因素。论文从影响土地资源部门配置的社会经济因素出发,构建了一个农地非农化经济驱动机制的理论分析框架,并应用1995～2001年省级数据进行实证分析。初步分析结果表明,人口增长、固定资产投资是农地非农化的主要推动因素;土地利用的比较效益和耕地资源禀赋是农地非农化的基础因素;地方政府的收益以及地方政府的管制应对行为与农地非农化呈正相关关系,而土地的市场化配置程度与农地非农化的面积呈负相关关系。根据上述的研究结果,文章提出了相应的政策建议。     

厌氧氨氧化混培菌的获得及其运行条件

采用了好氧活性污泥和厌氧颗粒污泥混合接种的方法 ,成功地启动了实验室规模的厌氧氨氧化反应器 ,启动后含氨模拟废水运行的进水氨浓度和进水亚硝基氮浓度均为 2 0 mmol/ L ,氨氮、亚硝基氮和总氮的容积负荷率为10 .69mmol/ L·d,12 .2 6mmol/ L· d和 3 94.5 5 mg/ L·d,氨氮、亚硝基氮和总氮的去除率保持在 90 %、99%和 95 %以上 ,对运行条件研究表明 ,厌氧氨氧化反应的最适 p H为 7～ 7.5 ,最适温度约在 3 0± 1℃。厌氧氨氧化随亚硝酸盐浓度的升高而下降 ,氨的厌氧转化随 COD浓度的增加也呈抑制型曲线 ,当 COD浓度为 80 0± 5 0 mg/ L 时 ,厌氧氨氧化速率达到最大     

An inhibitor of urease activity effectively reduces ammonia emissions from soil treated with urea under Mediterranean conditions

Urea is an important source of ammonia (NH₃) emissions to the atmosphere from agricultural soils. Abatement strategies are necessary in order to achieve NH₃ emission targets by reducing those emissions. In this context, a field experiment was carried out on a sunflower crop in spring 2006 with the aim of evaluating the effect of the N-(n-butyl) thiophosphoric triamide (NBPT) in the mitigation of volatilized NH₃ from a urea-fertilised soil. Ammonia emission was quantified, using the integrated horizontal flux (IHF) method, following application of urea with and without the urease inhibitor NBPT. Urea and a mixture of urea and NBPT (0.14%, w/w) were surface-applied at a rate of 170 kg N ha⁻¹ to circular plots (diameter 40 m). The soil was irrigated with 10 mm of water just after the application of urea to dissolve and incorporate it into the upper layer of soil. Over the duration of the measurement period (36 days) three peaks of NH₃ were observed. The first peak was associated with hydrolysis of urea after irrigation and the others with the increase of ammonia in soil solution after changes in atmospheric variables such as wind speed and rainfall. The total NH₃ emission during the whole experiment was 17.3 ± 0.5 kg NH₃–N ha⁻¹ in the case of urea treated soils and 10.0 ± 2.2 kg NH₃–N ha⁻¹ where NBPT was included with the urea (10.1 and 5.9%, respectively, of the applied urea–N). The lower NH₃ emissions from plots fertilised with urea + NBPT, compared with urea alone, were associated with a reduction in urease activity during the first 9 days after inhibitor application. This reduction in enzymatic activity promoted a decrease in the exchangeable NH₄⁺ pool.     

220kV输变电工程的环境影响评价

本文以＂鄂尔多斯东胜北郊变至布日都变第二回220kV输变电工程＂为例,对其环境现状进行了监测,分析研究了220kV输变电工程的环境影响,主要包括电磁环境、声环境和非污染生态三方面的影响。分析结果表明,本工程的各项环境监测指标均在标准限值范围内,对电磁环境监测结果的分析表明,增加导线对地距离或改变导线布置方式,有利于减小电磁场强度值。最后提出了相应的污染防治对策措施。