苯并芘和邻苯二甲酸二(2-乙基己基)酯复合暴露对稀有鮈鲫的内分泌干扰效应研究

为评价典型有机污染物共存情况下对长江上游三峡库区特有珍稀鱼类的环境风险,以稀有鮈鲫(Gobiocypris rarus)为对象,研究了多环芳烃和酞酸酯的代表物苯并芘(BaP)和邻苯二甲酸二(2-乙基己基)酯(DEHP)单独及复合暴露而引起的内分泌干扰效应。将成年稀有鮈鲫暴露于不同浓度的BaP(0.1、1μg·L~(-1))、DEHP(10、100μg·L~(-1))和BaP+DEHP((0.1+10)、(1+100)μg·L~(-1))中28 d后,测定了体质系数、性激素含量、下丘脑-垂体-性腺(HPG)轴和肝脏中与生殖相关基因的表达。结果表明:在雌鱼中,单独BaP或DEHP暴露对鱼体性激素含量、基因表达量无显著影响; BaP和DEHP复合暴露时,高浓度组睾酮(T)含量升高但雌二醇(E2)含量下降,fsh、vtg、 cyp17和17β-hsd基因表达量上调,cyp19a则下调,表现出协同毒性。因而雌鱼中性激素水平的改变可能与类固醇合成相关基因表达量的改变有关。而无论在单独暴露或是复合暴露情况下,对雄鱼体内性激素无显著影响。以上检测到的激素或基因表达量的改变主要发生在高剂量的复合暴露组中,而长江上游三峡库区水体中多环芳烃和酞酸酯含量低于暴露的最低浓度,因此推测,库区存在的多环芳烃和酞酸酯同时作用时不会对三峡库区上游水体中的鱼类产生生殖内分泌干扰效应。     

Evaluation of the use of bioaccumulation and biological effects tools in caged mussels,within the European Water Framework Directive

Bioaccumulation and biological effects of pollution were assessed in mussels (Mytilus galloprovincialis) caged for one month at three sites in the Oiartzun estuary (south-eastern Bay of Biscay, Spain) with the aim of evaluating their usefulness within the investigative monitoring defined in the European Union Water Framework Directive (WFD). The highest concentrations of organic contaminants determined in mussels' tissue were detected towards the inner part of the estuary but no gradient pattern was found for metal bioaccumulation. Population fitness responses measured as condition index, stress on stress and gonad index were similar in all caged mussels and did not follow the organic pollution gradient. However, biomarkers determined at tissue, cell and protein level (histopathology, micronuclei frequency, malondialdehyde levels and vitellogenin-like protein levels) revealed a higher stress syndrome at the inner part of the estuary showing signs of genotoxicity, oxidative stress and endocrine disruption. Overall, the integrated chemical–biological approach in connection with mussel caging technique proved to be a useful tool to assess environmental pollution, allowing a better understanding of the cause–effect relationship within the investigative monitoring defined in the WFD.     

微生物-化学水解联合作用下烟嘧磺隆的降解

从被烟嘧磺隆污染的人工湿地土壤中分离出1株能够在葡萄糖存在下降解烟嘧磺隆的微生物,通过16S rDNA序列同源相似性分析,初步鉴定该微生物为Klebsiella sp..它能够以烟嘧磺隆为唯一氮源生长,其最适生长条件为:温度35℃,初始pH为中性偏酸.水解试验表明,烟嘧磺隆在中性和碱性条件下比较稳定,在酸性条件下水解较快.生物降解试验发现,当培养液中葡萄糖浓度为5 g.L-1时,在温度35℃、初始pH为7的条件下培养10 d后烟嘧磺隆有99.4%得到降解,同时溶液的pH从7.0降低至4.0;而降低葡萄糖浓度分别为500 mg.L-1和100 mg.L-1时,培养10 d后烟嘧磺隆的降解率仅为11.7%和6.6%,溶液的pH始终在7左右.进一步研究表明烟嘧磺隆的降解是由于微生物代谢葡萄糖产生了酸性环境,pH降低引起了烟嘧磺隆水解,菌种对烟嘧磺隆降解的实质是微生物-化学水解联合作用.     

Toxicity assessing for chlorpyrifos-contaminated soil with three different earthworm test methods

Earthworm toxicity tests are useful tools for terrestrial risk assessment but require a hierarchy of test designs that differ in effect levels (behavior, sublethal, lethal). In this study, the toxicity of chlorpyrifos contaminated soil on earthworms was assessed. In addition to the acute and chronic tests, an avoidance response test was applied. Earthworms were exposed to sublethal and lethal concentration of chlorpyrifos, and evaluated for acute toxicity, growth, fecundity and avoidance response after a certain exposure period. The test methods covered all important ecological relevant endpoints (acute, chronic, behavioral). Concentration of 78.91 mg/kg, chlorpyrifos caused significant toxic effects in all test methods, but at lower test concentrations, only significant chronic toxic effects could be observed. In the present study, chlorpyrifos had adverse effect on growth and fecundity in earthworm exposed to 5 mg/kg chlorpyrifos after eight weeks. The avoidance response test, however, showed significant repellent effects concentration of 40 mg/kg chlorpyrifos. For chlorpyrifos, concentration affecting avoidance response was far greater than growth and fecundity, it seemed likely that earthworms were not able to escape from pesticide-contaminated soil into the clean soil in field and hence were exposed continuously to elevated concentrations of pesticides.     

基于生物导向的水生态健康监测及评估概述

环境监测是水生态健康监测与评估的重要环节,基于物理、化学监测的传统水质监测通常仅能提供独立的数据信息,不能全面、直观地反映水环境状况。基于生物等生命体导向的水生态监测通过生物对环境的响应,能够直接反应复杂水体状况,在水环境健康监测与评估中占据重要地位。基于病原微生物、指示生物介绍了生物监测中的常规生物指标,总结了包括藻类、无脊椎动物和鱼类在内的常见指示生物在不同类型污染水体中的环境指示作用。从生物毒性效应出发介绍了常用的毒性效应测试方法、分析了污染物在不同生物学水平的响应,从而指明生物毒性效应在水环境健康评估中的发展优势。再从生态完整性角度阐述了生态完整性评价的一般方法和新兴分子生物学技术在水生态健康评估中的应用。重点指出环境毒理学和分子生物学在水环境监测的优势,以期为更加科学精确地进行水生态健康监测预警提供支撑。     

基坑开挖与邻近高层建筑施工耦合模拟研究

以一邻近在建观光楼的基坑工程为背景,采用有限元软件MIDAS GTS-NX对其进行基坑开挖及邻近观光楼全过程数值模拟分析,结合模拟计算分析了邻近在建高层建筑的基坑开挖的耦合作用.模拟结果表明:在邻近在建建筑物的基坑开挖情况下,两者间的耦合作用明显;在邻近在建高层建筑物影响下,基坑支护结构水平位移差为10.1 mm;土体...     

Ecological principles,biodiversity, and the electric utility industry

The synthetic field of conservation biology uses principles derived from many different disciplines to address biodiversity issues. Many of these principles have come from ecology, and two simple ones that seem to relate to many issues involving the utility industry are: (1) Everything is interconnected (and should usually stay that way), and (2) We can never do merely one thing. The first principle can be applied to both the biotic and physical environments that are impacted by industrial activities. Habitat fragmentation and the loss of physical and biotic connectedness that results are frequently associated with transmission rights-of-way. These problems can be reduced—or even turned into conservation benefits—by careful planning and creative management. The second principle applies to the utility industry's programs to deal with carbon released by burning fossil fuels. Ecological knowledge can allow these programs to contribute to the preservation of biodiversity in addition to addressing a pollution problem. Without careful ecological analyses, industry could easily create new problems while implementing solutions to old ones.     

噪声对不同人格类型被试者视觉功能的不同影响

采用龚耀先修订的“艾克森人格问卷”中的调查方法,将华东师范大学的３６名大学生分为外向稳定、内向稳定、外向不稳定、内向不稳定等４个人格类型组,通过噪声暴露试验,研究其对不同人格类型式者视觉功能的影响。结果表明,噪声对内向不稳定定型被试乾的视觉影响最大,对外向稳定型影响最小;噪声对视深、视长和视野的影响程序也不相同,４种人格类型被试者在视深知觉和视野上均存在显著性差异,在视长智觉上未见任何显著性差异;     

森林与雾露水关系研究进展

论文从雾露水的形成机制、森林对雾露水的截留量、雾露水对森林分布的影响及雾露水的生态效应方面,阐述了国外在该领域的研究动态和取得的成果,以期推动我国在该领域的研究工作。国外的大量研究表明,雾露水对于植物的生长、分布具有重要的生态意义,是森林生态系统水分平衡、养分循环不可忽视的输入项,其生态效应是多方面的。因而,进一步深入研究雾露水与森林的关系是必要的。     

硒化kappa-卡拉胶对烟民生理损伤的修复作用

本实验选择19例年龄在39—59之间,烟史为15—30a,每日吸烟量为6—20支的男性为对象,连续服用硒化kappa-卡拉胶(100ug硒/d)三个月后,对几项指标的测定结果表明:微核、双链DNA及血硒水平均有明显差异,服硒前,19例烟民中微核淋巴细胞出现率为73.7％,淋巴细胞微核出现率为1.26±1.04‰,服硒后,微核淋巴细胞出现率为42.1％,淋巴细胞微核出现率为0.52±0.70‰,P<0.01;服硒前,双链DNA的含量为7.76±2.59％,服硒后增至10.5±2.19％,P<0.01;血硒水平变化更为明显,由服硒前的76.4±15.1ppb增至服硒后的148.3±44.1ppb,P<0.01.全部病例无任何毒、副反应。实验结果初步表明:硒化kappa-卡拉胶对烟民的生理损伤具有一定的修复作用。