依据(代)生物体对化学品的反应预测对生态系统服务功能的影响

保护生态系统服务功能越来越多地被作为风险评估的目标,但是目前生态风险评估的终点和评估生态系统服务功能受到的潜在影响之间有很大的差距。作者提出了一个框架,将常用的生态毒理学终点与对种群和群落的影响以及生态系统的服务功能联系起来。这个框架建立在机制效应模型的长足进步上,这些模型旨在跨越多种生物组织,并解释各种生物相互作用和反馈。为了说明这一点,作者引入了2个研究案例,它们采用了已完善和已验证的机制效应模型：鱼种群的inSTREAM个体模型和AQUATOX生态系统模型。他们还展示了动态能量平衡理论可以为解释组织级毒性提供一种通用货币。他们认为,一个基于机制模型的框架,可以预测化学品暴露对生态系统服务的影响,再结合经济估值,可以为环境管理提供一种有用的方法。作者强调了使用这个框架的潜在好处以及未来工作中需要解决的挑战。
精选自Forbes, V. E., Salice, C. J., Birnir, B., Bruins, R. J.F., Calow, P., Ducrot, V., Galic, N., Garber, K., Harvey, B. C., Jager, H., Kanarek, A., Pastorok, R., Railsback, S. F., Rebarber, R. and Thorbek, P. (2017), A framework for predicting impacts on ecosystem services from (sub)organismal responses to chemicals. Environmental Toxicology and Chemistry, 36: 845–859. doi: 10.1002/etc.3720
详情请见http://onlinelibrary.wiley.com/doi/10.1002/etc.3720/full
     

农药内分泌干扰效应研究进展

农药作为农业生产过程中的一类重要生产资料,主要用作杀虫剂、杀菌剂和除草剂等。由于农药的大量使用,目前在各类农产品及各种环境介质中都能被检测到,对饮食健康和环境安全带来潜在危害。研究发现,许多农药为内分泌干扰物,其能够干扰人类和其他生物体的内分泌系统正常功能。本文在总结国内外相关研究的基础上,对农药内分泌干扰效应的作用机理及筛选模型进行总结,对有机氯、有机磷和拟除虫菊酯等各类农药的内分泌干扰效应研究进展进行综述,并提出深入探究农药致毒作用机制和低剂量下多种农药联合毒性是本领域的重要研究方向。     

氯代阻燃剂得克隆对纤细裸藻的生态毒性效应

得克隆(Dechlorane Plus,DP)作为全球广泛使用的氯代阻燃剂,具有POPs特性和环境毒性,但其生物毒性数据非常有限。本文选择水生初级生产者纤细裸藻(Euglena gracilis)作为研究对象,通过检测藻细胞生长状况、光合色素水平、抗氧化酶活性、谷胱甘肽(GSH)和丙二醛(MDA)含量变化,研究了不同浓度DP对其生态毒性效应的影响。结果显示,低浓度DP对纤细裸藻生长具有一定促进作用,但差异不显著;DP浓度较低时(0.1和0.5 mg·L-1DP)类胡萝卜素含量受到轻微抑制;较高浓度DP暴露导致超氧化物歧化酶(SOD)、过氧化物酶(POD)和谷胱甘肽(GSH)水平显著增加,同时高浓度DP(8 mg·L-1)下MDA含量显著上升,提示膜结构受到损伤;表明较高实验浓度范围内,DP对纤细裸藻的生长及抗氧化系统均产生一定影响,结果将为DP的环境生态风险评价提供科学依据。     

全氟辛酸(PFOA)对菲律宾蛤仔体内酶活性的影响

采用半静态毒性实验方法,将菲律宾蛤仔(Ruditapes philippinarum)分别暴露于0.2、2、20μg·L-1的全氟辛酸(perfluorooctanoic acid,PFOA)中,在处理后第1、3、6、10、15、21天分别取样,测定整体组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、谷胱甘肽-S-转移酶(GST)、7-乙氧基异吩噁唑酮脱乙基酶(EROD)活性和过氧化脂质(LPO)含量。酶活性分析结果显示:PFOA对菲律宾蛤仔组织SOD、CAT和POD活性均呈现先促进后抑制的作用;低浓度组SOD活性在暴露第1天达到最高,显著高于对照组(P<0.01);中高浓度组SOD活性在暴露第6天达到最低;暴露1~15 d,低浓度组CAT活性均显著高于对照组(P<0.05);高浓度组CAT活性在暴露第6天得到显著诱导,其余时间基本处于抑制状态;中浓度组POD活性在暴露第3天即达到最高,高浓度组POD活性基本一直处于抑制状态;随着PFOA暴露时间的延长,菲律宾蛤仔组织LPO含量呈现了先降低后升高的趋势;各浓度组中EROD的活力都显著被诱导(P<0.01),与处理浓度呈正相关;中高浓度组的GST活性在胁迫期间变化比较显著,呈现诱导-抑制的变化规律。研究表明,PFOA暴露能够引起菲律宾蛤仔组织抗氧化酶和生物转化酶的变化,可以与其他敏感性指标一起作为指示早期海洋PFOA污染的生物标志物。     

Sorption and desorption of glyphosate,MCPA and tetracycline and their mixtures in soil as influenced by phosphate

Phosphate fertilizers and herbicides such as glyphosate and MCPA are commonly applied to agricultural land, and antibiotics such as tetracycline have been detected in soils following the application of livestock manures and biosolids to agricultural land. Utilizing a range of batch equilibrium experiments, this research examined the competitive sorption interactions of these chemicals in soil. Soil samples (0-15 cm) collected from long-term experimental plots contained Olsen P concentrations in the typical (13 to 20 mg kg^?1) and elevated (81 to 99 mg kg^?1) range of build-up phosphate in agricultural soils. The elevated Olsen P concentrations in field soils significantly reduced glyphosate sorption up to 50%, but had no significant impact on MCPA and tetracycline sorption. Fresh phosphate additions in the laboratory, introduced to soil prior to, or at the same time with the other chemical applications, had a greater impact on reducing glyphosate sorption (up to 45%) than on reducing tetracycline (up to 13%) and MCPA (up to 8%) sorption. The impact of fresh phosphate additions on the desorption of these three chemicals was also statistically significant, but numerically very small namely < 1% for glyphosate and tetracycline and 3% for MCPA. The presence of MCPA significantly reduced sorption and increased desorption of glyphosate, but only when MCPA was present at concentrations much greater than environmentally relevant and there was no phosphate added to the MCPA solution. Tetracycline addition had no significant effect on glyphosate sorption and desorption in soil. For the four chemicals studied, we conclude that when mixtures of phosphate, herbicides and antibiotics are present in soil, the greatest influence of their competitive interactions is phosphate decreasing glyphosate sorption and the presence of phosphate in solution lessens the potential impact of MCPA on glyphosate sorption. The presence of chemical mixtures in soil solution has an overall greater impact on the sorption than desorption of individual organic chemicals in soil.     

水培实验中不同粒径纳米TiO_2对芦苇种子发芽和植株生长和生理的影响

纳米二氧化钛(TiO_2-NPs)是目前应用最为广泛的纳米材料之一,进入到天然湿地和人工湿地中的TiO_2-NPs会在湿地生物、基质、水体之间进行迁移转化和归趋。目前针对TiO_2-NPs对湿地植物芦苇的毒性研究很少。本文采用水培实验方法,研究了不同粒径TiO_2对芦苇种子发芽和植株生长的影响,解析了TiO_2-NPs对芦苇的生态毒理效应。研究结果表明:几种粒径的TiO_2-NPs在较低浓度(≤200 mg·L~(-1))下对芦苇种子发芽均具有一定的促进作用,高浓度处理会抑制芦苇种子发芽;当TiO_2-NPs浓度为500 mg·L~(-1)时,处理后的芦苇种子的发芽指标较空白对照均出现显著降低,4 nm和20 nm的TiO_2-NPs对芦苇种子发芽的半数有效浓度(EC50)分别为1 075 mg·L~(-1)和1 680 mg·L~(-1)。TiO_2-NPs对芦苇植株的毒害作用表现为植株生长速度减缓甚至停滞、叶片失绿、植株萎焉或枯死、芦苇倒二叶面积增长率降低,并且TiO_2-NPs的粒径越小、浓度越高毒害作用越明显。随TiO_2-NPs浓度升高,处理后芦苇叶片的叶绿素含量和植株体内的Mg含量均降低,根系的超氧化物歧化酶(superoxide dismutase,SOD)活性、丙二醛(malondialdehyde,MDA)均升高,而且越小粒径的TiO_2-NPs对芦苇的处理效果越明显。3种粒径的TiO_2-NPs均能进入芦苇体内,粒径越小、浓度越高进入的量越多,但是TiO_2-NPs比较难以从根迁移转运至茎和叶。     

Back-pedaling or continuing quietly? Assessing the impact of ICLEI membership termination on cities’ sustainability actions

Over the past decade, cities have emerged as leaders in sustainability and climate protection in the United States. ICLEI, a voluntary network of local governments, played an important role driving this trend. After years of steady growth, ICLEI became a target of political opposition and its membership dropped significantly from 2010 to 2012. This begs the question of whether cities’ termination of their ICLEI affiliation diminishes their implementation of sustainability actions. Two surveys administered in 2010 and 2014 provide data on cities’ implementation of an array of sustainability actions. Using a difference-in-differences (DiD) method, the impact of ICLEI termination on local governments’ administrative and policy commitments to sustainability is assessed. The results suggest that ending ICLEI membership does not significantly impact local sustainability actions, and also indicate that the durability of policy actions may be only loosely linked to the policies that justify them.     

Transcriptional responses of heat shock protein 70 (Hsp70) to thermal, bisphenol A, and copper stresses in the dinoflagellate Prorocentrum minimum

The designation of biodiesel as an environmental-friendly alternative to diesel oil has improved its commercialization and use. However, most biodiesel environmental safety studies refer to air pollution and so far there have been very few literature data about its impacts upon other biotic systems, e.g. water, and exposed organisms. Spill simulations in water were carried out with neat diesel and biodiesel and their blends aiming at assessing their genotoxic potentials should there be contaminations of water systems. The water soluble fractions (WSF) from the spill simulations were submitted to solid phase extraction with C-18 cartridge and the extracts obtained were evaluated carrying out genotoxic and mutagenic bioassays [the Salmonella assay and the in vitro MicroFlow® kit (Litron) assay]. Mutagenic and genotoxic effects were observed, respectively, in the Salmonella/microsome preincubation assay and the in vitro MN test carried out with the biodiesel WSF. This interesting result may be related to the presence of pollutants in biodiesel derived from the raw material source used in its production chain. The data showed that care while using biodiesel should be taken to avoid harmful effects on living organisms in cases of water pollution.     

Biphasic effects of lanthanum on Vicia faba L. seedlings under cadmium stress, implicating finite antioxidation and potential ecological risk

In the present study, lanthanum (La) as a representative REE was used to explore the mechanisms for alleviation of Cd-induced oxidative damage by extraneous La at appropriate concentrations, and to assess ecological risk of combination of Cd and La at higher concentrations in roots of Vicia faba L. seedlings. The seedlings were hydroponically cultured for 15 d under nutrient solution, 6 μmol L⁻¹ CdCl₂, and combination of 6 μmol L⁻¹ CdCl₂ and increasing concentrations of La, respectively. The results showed that the supplementation with low concentrations of exogenous La (<120 μmol L⁻¹) led to reduced contents of Cd, Ca, Cu, Zn, Mn or Fe element and increased activities of superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX) and ascorbate peroxidase (APX) isozymes as well as heat shock protein 70 (HSP 70) production in the roots. However, the supplementation with higher La (>120 μmol L⁻¹) showed the adverse effects. The contents of Cd elevated above the single Cd treatment in the roots, accompanying with the decline of antioxidant isozyme’s activities and HSP 70, and increment of carbonylated proteins and endoprotease isozyme’s activities. The results also showed that the root growth was not only related to carbonylated proteins, but also to indole acetic acid oxidase activities. Therefore, the supplemented extraneous La contributed to biphasic effects: stimulated antioxidation at lower concentrations and pro-oxidation at higher concentrations against Cd-induced oxidative stress in the roots.     

基本农田保护政策的耕地保护效果评价

为分析基本农田保护政策对耕地保护的效果,论文把目标分解为两个问题,一是基本农田保护政策的实施是否对耕地流失面积的减少产生了影响;二是如果有显著影响,效果有多大,即因基本农田保护政策的实施,耕地流失面积减少了多少。论文收集了2004-2007年的省级面版数据,分别采用了固定效应模型、随机效应和混合OLS模型加以估计,固定效应和随机效应检验结果表明,固定效应和随机效应模型均不优于混合OLS模型。在模型估计结果的基础上,论文分析了基本农田保护政策对耕地流失的影响,并计算了基本农田保护政策的耕地保护效果。分析表明:①在2004-2007年期间,基本农田保护政策的实施对于减少耕地流失面积产生了显著影响,基本农田保护率每提高1%,每省每年平均耕地流失面积约能减少364.4 hm2;②由于基本农田保护政策的实施,2004-2007年期间,全国耕地流失面积分别减少了853 445.19 hm2,847 724.91 hm2,859 559.37 hm2和857 961.26 hm2,4年合计减少耕地流失面积3 418 690.73 hm2,约为期间实际耕地流失面积3 020 769.49 hm2的1.13倍。