Behavioral genomics of honeybee foraging and nest defense

The honeybee has been the most important insect species for study of social behavior. The recently released draft genomic sequence for the bee will accelerate honeybee behavioral genetics. Although we lack sufficient tools to manipulate this genome easily, quantitative trait loci (QTLs) that influence natural variation in behavior have been identified and tested for their effects on correlated behavioral traits. We review what is known about the genetics and physiology of two behavioral traits in honeybees, foraging specialization (pollen versus nectar), and defensive behavior, and present evidence that map-based cloning of genes is more feasible in the bee than in other metazoans. We also present bioinformatic analyses of candidate genes within QTL confidence intervals (CIs). The high recombination rate of the bee made it possible to narrow the search to regions containing only 17–61 predicted peptides for each QTL, although CIs covered large genetic distances. Knowledge of correlated behavioral traits, comparative bioinformatics, and expression assays facilitated evaluation of candidate genes. An overrepresentation of genes involved in ovarian development and insulin-like signaling components within pollen foraging QTL regions suggests that an ancestral reproductive gene network was co-opted during the evolution of foraging specialization. The major QTL influencing defensive/aggressive behavior contains orthologs of genes involved in central nervous system activity and neurogenesis. Candidates at the other two defensive-behavior QTLs include modulators of sensory signaling (Am5HT ₇ serotonin receptor, AmArr4 arrestin, and GABA-B-R1 receptor). These studies are the first step in linking natural variation in honeybee social behavior to the identification of underlying genes.     

Response thresholds to sucrose predict foraging division of labor in honeybees

Division of labor, where thousands of individuals perform specific behavioral acts repeatedly and non-randomly, is the hallmark of insect societies. Virtually nothing is known about the underlying neurophysiological processes that direct individuals into specific behavioral roles. We demonstrate that sensory-physiological variation in the perception of sucrose in honeybees measured when they are 1 week old correlates with their foraging behavior 2–3 weeks later. Workers with the lowest response thresholds became water foragers, followed with increasing response thresholds by pollen foragers, nectar foragers, bees collecting both pollen and nectar, and finally those returning to the colony empty (water<pollen<nectar<both<empty). Sucrose concentrations of nectar loads were positively correlated with response thresholds measured on 1-week-old bees. These results demonstrated how the variable response thresholds of a sensory-physiological process, the perception of sucrose, is causally linked to the division of labor of foraging. Received. 28 June 1999 / Received in revised form: 2 November 1999 / Accepted: 20 November 1999     

Abundance, behavior, and movement patterns of western gray whales in relation to a 3-D seismic survey, Northeast Sakhalin Island, Russia

A geophysical seismic survey was conducted in the summer of 2001 off the northeastern coast of Sakhalin Island, Russia. The area of seismic exploration was immediately adjacent to the Piltun feeding grounds of the endangered western gray whale (Eschrichtius robustus). This study investigates relative abundance, behavior, and movement patterns of gray whales in relation to occurrence and proximity to the seismic survey by employing scan sampling, focal follow, and theodolite tracking methodologies. These data were analyzed in relation to temporal, environmental, and seismic related variables to evaluate potential disturbance reactions of gray whales to the seismic survey. The relative numbers of whales and pods recorded from five shore-based stations were not significantly different during periods when seismic surveys were occurring compared to periods when no seismic surveys were occurring and to the post-seismic period. Univariate analyses indicated no significant statistical correlation between seismic survey variables and any of the eleven movement and behavior variables. Multiple regression analyses indicated that, after accounting for temporal and environmental variables, 6 of 11 movement and behavior variables (linearity, acceleration, mean direction, blows per surfacing, and surface-dive blow rate) were not significantly associated with seismic survey variables, and 5 of 11 variables (leg speed, reorientation rate, distance-from-shore, blow interval, and dive time) were significantly associated with seismic survey variables. In summary, after accounting for environmental variables, no correlation was found between seismic survey variables and the linearity of whale movements, changes in whale swimming speed between theodolite fixes, mean direction of whale movement, mean number of whale exhalations per minute at the surface, mean time at the surface, and mean number of exhalations per minute during a whales surface-to-dive cycle. In contrast, at higher received sound energy exposure levels, whales traveled faster, changed directions of movement less, were recorded further from shore, and stayed under water longer between respirations.     

Dietary exposure to technical hexabromocyclododecane (HBCD) alters courtship, incubation and parental behaviors in American kestrels (Falco sparverius)

Hexabromocyclododecane (HBCD) is a high production volume brominated flame retardant that has been detected in the environment and wildlife at increasing concentrations. This study was designed to determine potential effects of dietary exposure to environmentally relevant levels of HBCD on behavior during reproduction in captive American kestrels. Twenty kestrel pairs were exposed to 0.51 μg technical HBCD g⁻¹ kestrel d⁻¹ from 4 weeks prior to pairing until chicks hatched (∼75 d). Ten pairs of controls received the safflower oil vehicle only and were used for comparison. During the courtship period the chitter-calls were reduced in both sexes (p = 0.038) and females performed fewer bonding displays (p = 0.053). Both sexes showed a propensity to be less active than controls during courtship. The reduction in male courtship behavior was correlated with reduced courtship behaviors of females (p ? 0.008) as well as reduced egg mass (p = 0.019). During incubation, nest temperatures of treatment pairs were lower at mid-incubation (p = 0.038). HBCD-exposed males performed fewer key parental behaviors when rearing nestlings, including entering the nest-box, pair-bonding displays and food-retrievals. HBCD-exposed females appeared to compensate for the reduced parental behavior of their mates by performing these same behaviors more frequently than controls (p = 0.004, p = 0.027, p = 0.025, respectively). This study demonstrates that HBCD affects breeding behavior in American kestrels throughout the reproductive season and behavioral alterations were linked to reproductive changes (egg size). This is the first study to report HBCD effects on reproductive behavior in any animal model.     

Measuring determinants of occupational health related behavior in Flemish farmers: an application of the Theory of Planned Behavior

PROBLEM: Preventive interventions to reduce occupational injuries and health problems in farmers require the identification of factors that contribute to unsafe and health damaging behavior. This paper describes the development and validation of a self-report questionnaire, which measures the determinants of occupational health-related behaviors in farmers. METHOD: A representative sample of 283 Flemish farmers completed a provisional 135 item questionnaire based on the Theory of Planned Behavior, measuring four behaviors related to occupational health (machinery use, animal handling, preventing falls, and pesticide use), as well as the intentions, attitudes, perceived social norms, and self-efficacy for each of these behaviors. RESULTS: The fit indices of the Confirmatory Factor Analysis turned out not to be sufficient to reproduce the dimensions of the TPB. Therefore exploratory factor analysis was use to determine the underlying dimensions. Principal Component Analyses (PCA) on the behavioral items yielded single component solutions explaining a considerable proportion of the variance for each behavior and for behavioral intentions. Principal component analyses toward an a priori three-component structure reflecting the TPB dimensions did not produce sufficient congruence for the determinants of the four behaviors. Subsequent Varimax rotations and discarding of redundant items resulted in three component solutions explaining 50% to 69% of the variance in the determinants of each behavior, corresponding with the dimensions of the TPB. Internal consistencies ranged from .25 to .89. Scale scores accounted for a significant proportion of the variance in intention and self-reported behavior. IMPACT: The study demonstrates the validity of the TPB in predicting behavior related to occupational safety and health in farmers, and provides a valid and reliable questionnaire to measure the cognitive concepts featured in this model. IMPACT ON INDUSTRY: Both authors share the same view on this study's impact on industry. In recent years, efforts have been made to create awareness among farmers about occupational hazards, and to encourage farmers to perform safer and healthier behavior. However, only a limited number of these interventions have proven to be successful. A possible reason for this relative lack of success is that interventions typically focus on risk analysis and raising awareness, whereas the literature on preventive health behavior change indicates that knowledge and awareness with regard to possible health risks are neither necessary nor sufficient to change behavior. To raise the effectiveness of prevention programs, other relevant determinants of behavior need to be addressed as well, such as: attitudes, perceived social norms, self efficacy, and elements of the physical environment that elicit or reinforce behavior. These determinants play a key role in psychological models of health related behavior, such as the Theory of Planned Behavior. Thus far, the use of these models within agricultural settings is fairly limited. This study demonstrates the validity of the Theory of Planned Behavior in predicting behavior related to occupational safety and health in farmers, and provides a valid and reliable questionnaire to measure the cognitive concepts featured in this model.     

Nickel sulfate induces numerous defects in Caenorhabditis elegans that can also be transferred to progeny

Whether the multiple biological toxicities from nickel exposure could be transferred to progeny has not been clarified. In this report, we explored the Caenorhabditis elegans to analyze the multiple toxicities of nickel and their possibly transferable properties. The nickel toxicity caused multiple biological defects in a concentration-dependent manner. Moreover, most of these toxicities could be transferred and could be only partially rescued in progeny. Some specific phenotypes in progeny were also found to exhibit no obvious rescue phenotypes or to show even more severe defects than their parents. The defects caused by nickel exposure could be classified into four groups according to their transferring properties. That is, the defects caused by nickel exposure could be largely, or partially, or unable to be rescued, or became even more severe in progeny animals. Therefore, most of the nickel exposure-caused defects can be transferred from parents to their progeny to different degrees in C. elegans.     

Behavior and lifestyle characteristics of male Kuwaiti drivers

Introduction: The high traffic accident risk among young drivers is a well-known and well-documented fact in most countries. Lifestyle has proven to affect driving behavior as well as accident risk. This study covers the lifestyle component of the problems related to young male Kuwaiti drivers’ accident risk. Methods: The purpose of the study is to measure the relationship between lifestyle and accident risk. Lifestyle is measured through a questionnaire, where 302 male Kuwaiti drivers (mean age = 28 years; range 25-35 years) answer 39 questions related to behavioral and social factors, road conditions, police enforcement, and life satisfaction. They also report their involvement in accidents and traffic violations. Results: The questionnaire's validity and reliability (Cronbach's alpha = 0.7) were achieved. Principal component analysis reduced the 39 items on the questionnaire to 5 factors. Inadequate police enforcement is strongly correlated (r = 0.862) to accident risk and traffic violations and is thus considered the best predictor of traffic accidents in Kuwait. Impact on Industry: As driving-related incidents (on-the-job and off-the-job) are a significant source of fatalities and lost-work-days, the study points to the importance of considering cultural factors in the design of comprehensive safety programs for industry.     

Evaluation of the Environmentalist Dimension of Ecotourism at the Dadia Forest Reserve (Greece)

Environmental education and financial support of nature conservation are considered among the primary components of the environmentalist dimension of ecotourism. The potential of environmental education calls for enhancing visitor environmental knowledge and prompting change of visitor behavior. The present study addressed the evaluation of the environmentalist dimension of ecotourism in the Dadia Forest Reserve. The first objective of the research was to study the influence of variables determined prior to the visit, namely, visitor and visit characteristics, visitor environmental information sources, visitor pro-environmental behavior, and visitation goals, on variables determined during the visit, that is, visitor participation in and satisfaction from ecotourism activities. The second objective was to study the effect of selected variables on visitor knowledge and behavior intentions. Visitor participation in and satisfaction from ecotourism activities were primarily controlled by visit characteristics. Levels of visitor knowledge were quite satisfactory; however, coherence among knowledge items was rather loose. On the other hand, behavior intention items revealed the highest possible degree of coherence, which should be attributed to the pervasive influence of visitor satisfaction from watching birds at the Bird Observatory within the Dadia Forest Reserve. Ecotourism activities, during which the main part of environmental knowledge was offered, exerted no effect on visitor knowledge; all the same, they significantly influenced visitor behavior intentions. Overall, visitor behavior intentions revealed a considerable potential of financing environmental protection and providing voluntary work in the frame of the environmental management of the Dadia Forest Reserve.     

The effects of size-dependent predation risk on the interaction between behavioral and life history traits in a stream-dwelling isopod

In field surveys, laboratory observations and field-based assays of behavior, I examined the effects of size-dependent predation risk on the interaction between size at reproductive maturity and maternal care behavior in the stream-dwelling isopod, Lirceus fontinalis. L. fontinalis exhibit population-specific sizes at reproductive maturity which result in population differences in predation risk during the adult phase. Females from streams containing salamander larvae (that prefer small prey) mature at large sizes and then become relatively safe from predation. Females from streams containing fish (that consume all size classes of prey equally) mature at small sizes and remain at risk. I tested whether these differences in expected survival were reflected in the behavior of females during the maternal phase (i.e., the period during which females exhibit maternal care). Female L. fontinalis carry developing juveniles inside a brood pouch. I simulated predatory attacks on gravid female L. fontinalis from the different population types and found that female behavior correlated with population differences in risk. When “attacked”, females from streams with predatory fish (that experience high risk to adult females) released juveniles from the brood pouch, whereas females from populations with predatory salamander larvae (that pose relatively little risk to adult females) did not release juveniles. I discuss the results with reference to the joint evolution of behavioral and life history traits. Received: 6 March 1996 /Accepted after revision: 12 August 1996