温度和C/N比对生物膜反硝化速率的影响

温度和C/N比是影响生物反硝化速率的2个重要参数,以反硝化生物膜为对象,研究了温度和C/N比对生物膜反硝化速率的影响.实验结果表明,生物膜在25 ℃下的反硝化速率要比13 ℃下的大;C/N比越高生物膜反硝化速率越大.     

Chromosomal genes conferring tolerance to heavy metal (Ag) toxicity

Bacterial strains were isolated from sediment samples from the Thames River. Successive transfer growth of the various strains on nutrient agar containing increasing concentrations of AgNO₃ revealed that three of the bacterial isolates were found to be capable of tolerating high concentrations of AgNO₃ ranging from 20 to 80 mM on a solid medium and up to 10 mM AgNO₃ in liquid medium. Molecular characterization and identification based on 16S rDNA gene sequencing of three strains of bacteria that are tolerant to silver nitrate showed that the major tolerant strains include the superbug, Shewanella oneidensis, Pseudomonas sp. and Bacillus sp. Protein extraction and two-dimensional (2D) sodium dodecyl sulfate SDS-polyacrylamide gel electrophoresis (PAGE) of the protein extracts in bacteria exposed to very high concentrations of AgNO₃ revealed a general reduction in the number of expressed proteins, although two protein spots were conspicuously over expressed in the exposed bacteria compared to control. The N-terminal amino acid sequence analysis of the protein spots identified the major up-regulated proteins as the outer membrane protein To1C (45.2 kDa) and the structural protein of the flagellar filament, flagellin (28.34 kDa), encoded for by the to1C and fliC genes, respectively. The roles of these genes in a number of multi-drug resistant pathogen and potentials for biotechnological applications in toxic metal control for treatment of contaminated ecosystems and biomining were discussed.     

近百年来长江荆江段牛轭湖沉积的孢粉、炭屑特征及其环境意义——以天鹅洲、尺八湖为例

根根据长江荆江段沿江牛轭湖天鹅洲和尺八沉积钻孔柱样,在建立了高分辨率的年代框架的基础上,分别进行了沉积物孢粉、炭屑分析,并结合荆州市1955~2012年的气候数据、史志记载的洪水和火灾等环境事件进行了综合分析。结果显示:天鹅洲、尺八两湖泊钻孔沉积物的孢粉和炭屑特征均较好地响应了近百年来湖区周边的环境变化;牛轭湖在裁断成湖之前、成湖之后的孢粉、炭屑特征不同:成湖之前,沉积物总孢粉浓度与乔木、灌木孢粉浓度都相对较低,水生植物和蕨类植物孢粉浓度相对较高,成湖之后乔木孢粉浓度仍呈减小趋势,但灌木、水生植物孢粉浓度呈波动增加趋势;利用沉积物炭屑浓度与孢粉浓度的比值(C/P值)指标,可以较好地指示自然山火与人类聚居地火灾等燃烧事件在沉积物中的记录,而且综合利用C/P值与沉积物孢粉特征,有可能厘清长江全流域洪水与局地洪涝事件的沉积记录。     

Steel consumption and economic growth in Korea: Long-term and short-term evidence

This paper examines the long-term and short-term causal relationships between steel consumption and economic activity in Korea between 1975 and 2008 using vector error correction and vector autoregression models. This study disaggregates steel products and steel-consuming industries because the consumption of a specific steel product is closely linked to the output of the corresponding industry. This approach can provide a clear and reliable causal relationship between variable pairs. The results show that total steel consumption and GDP have a long-term equilibrium relationship, running from GDP to total steel consumption. However, a long-term bi-directional causal relationship exists between flat products consumption and the manufacturing GDP. Flat products consumption also demonstrates the long-term relationships between steel-consuming industries, such as automobile, shipbuilding, and fabricated metal product, but causal directions are mixed, depending on variable pairs. These results imply that the reciprocal growth between these industries has contributed to the robust competitiveness of Korean manufacturing. Because Korea has maintained its export-oriented industrial policy based on manufacturing, the steel and steel-consuming industries are expected to play a significant role in economic growth far into the future.     

水中~（14）C取样与测量方法的实验研究

目前由于日本福岛核电事故,全世界高度关注核污染,14C作为影响人体健康8个主要放射性核素之一,在核电站的运行过程中随流出物大量排入周围的环境中,因此也受到人们的关注。作者在前人工作的基础上设计了一种采样及测定水中14C的分析方法,并确定出了该方法对水中碳取样效率、NaOH溶液对CO2捕集效率、CaCO3沉淀制备效率、液闪对Ca14CO3的探测效率等的最优化条件。实际测得探测下限为0.12 mBq/L。     

介孔涂层SPME- HPLC联用测定水样中的多环芳烃

以辛基键合SBA- 15为固相微萃取(SPME)的吸附涂层,考察了吸附和解吸时间、萃取温度、搅拌速率对SPME效率的影响,该方法的线性范围分别为0.4-300μg/L、4.0-450μg/L,检出限为0.3μg/L、0.10μg/L,以此方法测定了环境水样中萘、蒽的含量.该方法具有灵敏度高和精密度好的特点.     

烯酮还原酶基因的克隆与优化表达

烯酮/酯还原酶可以专一性地还原烯酮/酯中的碳碳双键,并引入两个手性中心,是手性化合物生物催化合成的重要酶类之一;在使用烯酮/酯还原酶进行全细胞手性分子生物催化合成中,其他还原酶的存在常导致副反应的产生.为研究烯酮/酯还原酶的理化性质、底物的专一性、产物的手性特点等,需要经过纯化的烯酮/酯还原酶.为此,根据烯酮还原酶(Enoate reductase,ERs,EC 1.3.1.31)的基因序列设计引物,以丙酮丁醇梭菌(Clostridium acetobutylicum)ATCC824基因组DNA为模板,通过PCR扩增技术得到了目标酶的基因,目的片段全长为1 995 bp,共编码664个氨基酸,相对分子质量为73×103.将烯酮还原酶基因连接到pET-32a(+)上,构建表达质粒pET-32a(+)-ERs,并成功在Escherichia coli Rosetta(DE3)pLysS中表达.在摇床上优化的表达条件为:诱导温度为18℃,诱导起始时菌体D600 nm为0.6～0.8,转速为100 r/min,诱导剂IPTG浓度为0.1 mmol/L,诱导培养时间为12 h,表达的烯酮还原酶大部分以可溶性形式存在于菌体内.     

紫花苜蓿改良盐渍土对土壤微生物活性和养分含量的影响

为探讨干旱地区种植紫花苜蓿改良盐渍土对土壤微生物生物量及其活性和土壤养分的影响,选择河西走廊黑河流域国有临泽农场的草甸盐土荒地作为对照,研究了种植紫花苜蓿2、3、4年后的改土效果。结果表明：与CK相比,连续种植4年后,所测定的土壤化学性质和微生物化学性质指标都发生了极显著（P〈0.01）的有利变化,电导率下降5.96 mS.cm-1,pH下降0.25,有机碳增加1.50 g.kg-1,微生物生物量碳增加48.93 mg.kg-1,微生物熵上升0.43%,荧光素二乙酸酯水解率提高10.87μg.g-1.h-1,碱解氮增加17.37 mg.kg-1,速效磷增加2.87 mg.kg-1,速效钾增加44.93 mg.kg-1。相关分析表明,微生物生物量碳、微生物熵、荧光素二乙酸酯水解率、碱解氮、速效磷、速效钾与土壤有机碳之间极显著（P〈0.01）正相关,相关系数分别为0.86、0.80、0.87、0.85、0.79、0.80,而与电导率之间极显著（P〈0.01）负相关,相关系数分别为-0.83、-0.79、-0.84、-0.86、-0.88、-0.78。研究认为,种植紫花苜蓿对草甸盐土有显著的改良效果,电导率下降,有机碳含量提高,微生物活性增强,速效养分含量增加。     

过氧化氢异丙苯热稳定性与热安全性研究

为研究过氧化氢异丙苯(CHP)的热稳定性和热安全性,利用C80微量量热仪对CHP在空气中的热分解进行试验研究。利用热分析技术研究CHP的热分解,得到了升温速率对CHP热分解的影响,CHP热分解的活化能,绝热条件下最大反应速率到达时间Tmrad和不同包装下的自加速分解温度。结果表明:随着升温速率的增加,CHP的起始放热温度和最大放热温度随之升高;CHP热分解的活化能范围为52～91 kJ/mol;Tmrad为1,8,24,50和100 h时对应的起始温度分别为118.08,75.41,55.83,44.83和34.52℃;CHP的储罐内径越大,其对应的自加速分解温度越低。     

机场围界入侵报警系统的设计

结合机场的地域特征,介绍一种检测人入侵行为的设计方案.以激光光电感应作为传感器件,通过对89052单片机进行C语言编程,检测和判断人入侵事件.通过大量实验获取样本时间点,利用聚类分析的算法有效排除掉噪声点,得到可靠样本空间,计算出可信赖样本阈值范围,经实验验证入侵判断误报率符合要求.