稀土金属离子对梨形四膜虫的24小时生长影响

应用４种方法（细胞直计计数法、中性红试验、重蛋白及核酸含量测定）研究不同浓度稀土金属离子Ｌａ＾２＋、Ｓｍ＾３＋、Ｙ＾３＋、Ｇｄ＾３＋处理２４ｈ后，对梨形四膜虫的生长影响，结果表明，在低浓度时Ｌａ＾３＋、Ｓｍ＾３＋、Ｙ＾３＋，对四膜虫具有刺激生长的作用。随着浓度的升高，４种稀土金属离子均表现出毒性效应；高浓度时，抑制生长艇特别明显。计算Ｌａ＾３＋、Ｓｍ＾３＋、Ｙ＾３＋、Ｇｄ＾３＋的半数抑制浓度（ＩＣ     

Effect of iron and dissolved silica on primmorphs of Petrosia ficiformis (Poiret, 1789)

The suitability of the primmorphs system as a good model for biotechnological applications led researchers on primmorphs to look for a medium to stimulate cell proliferation and therefore growth of aggregates. Recent efforts have focused on the use of Fe⁺³ and Si that, supplemented to cell culture medium, were found to be promising for growth and morphogenesis of the sponge Suberites domuncula. In this work, we analysed the effect of iron and dissolved silica on primmorphs of Petrosia ficiformis, by testing them at different concentrations in successive experiments. The purpose of these experiments was to test their effect on primmorphs and individuate their optimal concentration for this species. Our results suggest a negative effect of iron on primmorphs of P. ficiformis and a positive effect of silica on primmorphs size and spiculogenesis at a concentration of 120 μM.     

Investigation of nuclear enzyme topoisomerase as a putative molecular target of monohaloacetonitrile disinfection by-products

Disinfection by-products occur widely as the unintended effect of water disinfection and are associated with toxicity and adverse human health effects. Yet the molecular mechanisms of their toxicity are not well understood. To investigate the molecular basis of hyperploidy induction by monohaloacetonitriles, the interaction of monohaloacetonitriles with topoisomerase Ⅱ in Chinese hamster ovary cells was examined. We showed a concentration-dependent inhibition of DNA decatenation activity of topoisomerase under acellular conditions while in vitro monohaloacetonitrile treatment expressed mixed results. The working hypothesis, that topoisomerase Ⅱ is a molecular target of monohaloacetonitriles, was only partially supported.Nevertheless, this research serves as a starting point toward molecular mechanisms of toxic action of monohaloacetonitriles.     

Distracted walking: cell phones increase injury risk for college pedestrians

Introduction

Distraction on cell phones jeopardizes motor-vehicle driver safety, but few studies examine distracted walking. At particular risk are college students, who walk frequently in and near traffic, have increased pedestrian injury rates compared to other age groups, and frequently use cell phones. Method: Using an interactive and immersive virtual environment, two experiments studied the effect of cell phone conversation on distraction of college student pedestrians. In the first, we examined whether pedestrians would display riskier behavior when distracted by a naturalistic cell phone conversation than when undistracted. We also considered whether individual difference factors would moderate the effect of the distraction. In a second experiment, we examined the impact of three forms of distraction on pedestrian safety: (a) engaging in a cell phone conversation, (b) engaging in a cognitively challenging spatial task by phone, and (c) engaging in a cognitively challenging mental arithmetic task by phone. Results: Results revealed that cell phone conversations distracted college pedestrians considerably across all pedestrian safety variables measured, with just one exception. Attention to traffic was not affected by the naturalistic phone conversation in Experiment 1, but was altered by the cognitively-demanding content of some types of conversation in Experiment 2. The content of the conversation did not play a major role in distraction across other variables; both mundane and cognitively complex conversations distracted participants. Moreover, no significant associations between individual difference factors and susceptibility to distraction emerged. Impact on Industry: Results may inform researchers, policy makers, and pedestrians themselves. Educational campaigns might discourage telephone conversations in pedestrian environments.     

Spatiotemporal dynamics of cell abundance, colony size and intracellular toxin concentrations of pelagic and benthic Microcystis in Lake Caohai, China

Lake Caohai has experienced extensive Microcystis blooms in recent years,and to improve its water quality,the local government carried out a series of water control measures.To better understand the dynamics of both pelagic and benthic Microcystis and their characteristics in Lake Caohai,we conducted a 1-year investigation from December 2015to December 2016 to gain a seasonal outlook on the distribution and dynamics of cell abundance,colony size and intracellular microcystins(MCs)of Microcystis.The results indicated that the Microcystis bloom occupied primarily the northeastern region and then moved gradually from lakeshore to lake center.The perennial southwesterly winds and the water inflow from northeast to southwest in Lake Caohai determined the spatiotemporal distribution of pelagic Microcystis.Benthic Microcystis was mainly distributed in the northeastern region in summer,occupied the lake center in autumn and then occupied the southeastern region in winter,determined by the sedimentation of pelagic Microcystis and the death of benthic Microcystis.Small colonies(20-60μm)overwintered more easily in both water column and sediment.The concentrations of intracellular toxin of benthic Microcystis were observed to be significantly higher than those of pelagic Microcystis.This might be because Microcystis synthesized large amount of MCs to acclimate to an unfavorable benthic environment.This knowledge on the dynamics of Microcystis expands our understanding of mechanisms underpinning the formation of Microcystis blooms.     

Assessing combined toxic effects of tetracycline and P25 titanium dioxide nanoparticles using Allium cepa bioassay

• UVA pre-irradiation to TiO₂ NPs enhanced its toxicity toward plant A. cepa. • UVA TiO₂ NPs increased intracellular ROS, resulting in more cell damage. • Cell death enhanced cell permeability and increased uptake of NPs. • Being highly toxic (EC₅₀ = 0.097 µmol/L), TC did not increase ROS generation. • Even at a low dose, TC enhanced the toxic potential of TiO₂ NPs significantly. Usage of titanium dioxide nanoparticles (TiO₂ NPs) and tetracycline (TC) has increased significantly in the present era. This leads to their release and accumulation in the environment. Both the compounds, individually, can have adverse toxic effects on the plants. Their binary mixtures can increase this degree of damage. The present study aimed to evaluate the toxicity of both the contaminants in individual and binary mixtures in Allium cepa. Further, the toxicity of TiO₂ NPs upon UVA pre-irradiation was also measured. Results showed that UVA pre-irradiated NPs (UVA-TiO₂ NPs) had a significant decrease in cell viability than their non-irradiated counterparts (NI-TiO₂), denoting an increase in photocatalytic activity upon UVA pre-irradiation. Very low concentrations of TC (EC₁₀ = 0.016 µmol/L) mixed with TiO₂ NPs significantly increased the toxicity for both UVA-TiO₂ and NI-TiO₂ NPs. Intracellular ROS generation was significantly high for UVA-TiO₂ NPs. However, TC did not have any effects on ROS production. Both the compounds exhibited genotoxic potential in A. cepa. Different chromosomal abnormalities like anaphase bridges, telophase bridges, laggard chromosomes, binucleate cells, etc. were observed. The binary mixture of UVA-TiO₂ NPs and TC showed the highest chromosomal aberrations (64.0%±1.26%) than the mixture with NI-TiO₂ or the individual contaminants. This decreased significantly after recovery (46.8%±1.92%), denoting the self-repair processes. This study proved that UVA-TiO₂ NPs were more toxic and could be enhanced further when mixed with a sub-lethal concentration of TC. This work will help to assess the risk of both compounds in the environment.     

Ozone–dioxane delignification from the cell walls of Japanese cypress (Chamaecyparis obtusa Endl.)

Delignification from the cell walls with a combination of ozone oxidation and dioxane–water extraction using thin sections of a softwood, Japanese cypress (Chamaecyparis obtusa Endl.), was studied to determine its suitability for the production of recyclable cellulose-based materials from wood waste. The visible-light absorption spectra of treated wood sections revealed that delignification from the cell walls with ozone increased with increasing ozonization time. Ozone delignification proceeded from the lumen side toward the middle lamella within the secondary wall of a cell, and it proceeded faster in early wood than in late wood within an annual ring. Mild ozonization for 10–30 min was sufficient for the removal of lignin from the cell walls when sections were extracted with dioxane after ozonization. The results obtained here demonstrate that microspectrometry coupled with the Wiesner reaction is useful for the quantitative analysis of lignin in cell walls.     

三种典型的污泥发电工艺

污泥发电是城市污水处理厂进行污泥合理开发利用的技术措施之一,是污泥实行减量化、稳定化、无害化、资源化的良好方法,本文介绍了3种典型的污泥发电工艺：污泥焚烧发电;污泥厌氧消化产生沼气、通过燃气轮机组发电;污泥厌氧消化产生沼气、进而通过改质制造氢气,经燃料电池发电。对污泥合理利用的规范化、科学化有一定的借鉴意义。     

污水生物处理系统中内源过程的研究进展

污水生物处理系统的内源过程涉及到一系列微生物学、生态学机制和过程,因而对处理系统的各个方面都有重要影响.然而-目前对内源过程的认识还相当有限.本文首先在总结现有知识的基础上对污水生物处理系统中的内源过程作了定义,并对其区分为细胞和群落两个水平-随后,分别对内源过程中的细胞维持、解偶联、程序化细胞死亡、饥饿状态、高等微生物捕食等过程作了综述,总结了其在污水生物处理领域的研究进展.此外.文章还概述了细胞维持能量和细胞衰减速率的测定方法及其研究进展和存在的问题.本文的目的是通过综述现有的知识,推动对污水生物处理系统中内源过程研究的进一步发展.     

人工神经网络法在大气污染预报中的应用

以鞍山市为例,应用人工神经网络方法,模拟人脑的思维方式,建立了大气污染物浓度的神经网络预报模型,并将计算结果与监测值进行了对比验证,计算结果表明,BP模型应用于大气污染物浓度预报具有较高的预报精度。