Peroxidase activity,lignification and promotion of cell death in tobacco cells exposed to static magnetic field

Bio-effects of static magnetic fields on cell growth and cell death have been investigated in suspension-cultured tobacco cells as undifferentiated, embryonic plant cell model. The cells in their logarithmic growth phase were exposed to static magnetic field with the magnitudes of 10 mT and 30 mT for 5 h/day. Exposure to static magnetic field ceased the growth and caused an increase in cell death of exposed tobacco cells compared to those cells which were not treated with the field. Promotion of cell death was accompanied by a harmonized increase in the activity of peroxidase and increase of lignifcation of cell walls.     

Impact of the composition of combustion generated fine particles on epithelial cell toxicity: influences of metals on metabolism

Inhaled airborne particulate matter (PM) represents a potentially significant health hazard to humans. Exposure to PM strongly correlates with pulmonary inflammation and incidences of severe respiratory distress, including increased hospital admissions for breathing disorders, asthma, emphysema, and chronic bronchitis. PM generated from the combustion of fuel oils and coals contain a number of water-soluble transition metals including Fe, V, and Zn.

We have evaluated the impact of PM types with varying composition collected from the combustion of oils and coals on the health and metabolism of lung cell cultures. Three colorimetric assays (sulforhodamine B (SRB), Janus green, and MTT) have been adapted to quantify the impact of PM on rat lung alveolar type II epithelial cells (RLE-6TN cells). The PM toxicity metrics evaluated were inhibition of cell proliferation (SRB and Janus green) and inhibition of cellular metabolism (MTT). Cell proliferation is inhibited in a consistent dose-dependent manner by PM concentrations from 25 to 250 μg/ml. At a level of 100 μg/ml, oil-derived PM diminishes cell metabolism by as much as 40% relative to controls; the degree of inhibition is strongly dependent on PM particle size and metal content. Conversely, coal-derived PM at the same dosage diminishes cell metabolism by no more than 20% relative to controls. All three assays provide highly repeatable results and consistent toxicity rankings of the PMs evaluated. Overall, metabolic inhibition as measured by the MTT assay was deemed the most appropriate metric for PM toxicity, primarily due to its applicability with in vivo-like confluent cell monolayers.     

^3H的辐射生物学效应的研究——氚水对莱哈衣藻细胞分裂率的影响

摘要氚(^3H)为一种重要的放射性毒素。经氚水(HTO)在细胞内和细胞外照射后，莱哈衣藻细胞分裂率k值下降。HTO剂量越高，k值越小，在一定范围内照射时间越长，处理组的k值与对照组的k值相差越大。k值的变化与辐射剂量、辐射时间有相关性。     

烹调油烟对DNA和细胞的损伤

综述了烹调油烟的成分及对DNA和细胞的损伤，提出了降低居室内烹调油烟的措施。     

燃料电池汽车的环境意义

介绍了燃料电池的结构，特点和燃料电池动力汽车的发展概况；通过比较，阐述了燃料电池汽车的环境意义。     

EDTA对汞毒害下小麦幼苗细胞膜及体内保护系统的影响

研究表明，向含汞污水中加入ＥＤＴＡ能使汞引起的小麦幼苗细胞膜透性，膜脂质过氧化水平，ＳＯＤ活性，可溶性糖及可溶性蛋白质含量的增加和ＣＡＴ活性的降低得以恢复，汞对幼苗的毒害减轻。     

Contributions of root cell wall polysaccharides to Cu sequestration in castor (Ricinus communis L.) exposed to different Cu stresses

Cell wall polysaccharides play a vital role in binding with toxic metals such as copper(Cu)ions.However, it is still unclear whether the major binding site of Cu in the cell wall varies with different degrees of Cu stresses.Moreover, the contribution of each cell wall polysaccharide fraction to Cu sequestration with different degrees of Cu stresses also remains to be verified.The distribution of Cu in cell wall polysaccharide fractions of castor(Ricinus communis L.) root was investigated with various Cu concentrations in the hydroponic experiment.The results showed that the hemicellulose1(HC1) fraction fixed 44.9%–67.8% of the total cell wall Cu under Cu stress.In addition, the pectin fraction and hemicelluloses2(HC2) fraction also contributed to the Cu binding in root cell wall,accounting for 11.0%–25.9% and 14.1%–26.6% of the total cell wall Cu under Cu treatments, respectively.When the Cu levels were ≤ 25 μmol/L, pectin and HC2 contributed equally to Cu storage in root cell wall.However, when the Cu level was higher than 25 μmol/L, the ability of the pectin to bind Cu was easy to reach saturation.Much more Cu ions were bound on HC1 and HC2 fractions, and the HC2 played a much more important role in Cu binding than pectin.Combining fourier transform infrared(FT-IR) and twodimensional correlation analysis(2 D-COS) techniques, the hemicellulose components were showed not only to accumulate most of Cu in cell wall, but also respond fastest to Cu stress.     

大庆地区几种工业废水生物遗传毒性水平的测试与评价

文章报道了利用蚕豆根尖细胞微核技术测试石油开采、石油化工等几种工业废水的遗传毒性水平。经统计学分析,各排放口的废水均有不同程度的遗传毒性效应。并利用污染指数进行生物毒性等级的划分,从生物毒理学角度为行业污染源管理、控制污染物排放提供科学依据。     

Inhibition mechanism of fermentation broth extract of Phomopsis sp. Strain S4 on cell membranes of Magnaporthe oryzae北大核心CSCD

The secondary metabolites of endophytic Phomopsis sp. strain S4 show antifungal activity against a variety of plant pathogens, which implies that strain S4 has potential prospect in crop disease control. The aim of this study was to explore the inhibition mechanisms of S4 against plant pathogenic fungi. Magnaporthe oryzae was used as the main pathogenic material for the research. Cell membrane changes were detected using antifungal experiments, scanning electron microscopy, Q RT-PCR, and cell content leaking experiments. The results of scanning electron microscopy showed that M. oryzae mycelia, after treatment with S4 fermented product extract, decreased in size, suggesting the integrity of the cell membrane was destroyed. The genes related to ergosterol synthesis, which plays an important role in membrane integrity, were studied though Q RT-PCR. The results showed that the expression levels of ERG1, ERG11, and ERG6 genes were down-regulated by 2.0, 1.40, and 2.7-fold, respectively, whereas that of ERG7 was up-regulated by 1.38-fold, which means the ergosterol synthesis pathway was destroyed. The physiological experiments also showed that the cell contents of M. oryzae mycelia treated with S4 fermented product extract leaked significantly, which was consistent with the Q RT-PCR results. The results showed that S4 fermentation broth exact could destroy the cell membrane integrity by inhibiting ergosterol synthesis, and eventually inhibit M. oryzae cell growth. © 2018 Science Press. All rights reserved.     

Effects of four commonly used UV filters on the growth,cell viability and oxidative stress responses of the Tetrahymena thermophila

UV filters are increasingly used in sunscreens and other personal care products. Although their residues have been widely identified in aquatic environment, little is known about the influences of UV filters to protozoan. The growth inhibition effects, cell viability and oxidative stress responses of four commonly used UV filters, 2-ethylhexyl 4-methoxycinnamate (EHMC), benzophenone-3 (BP-3), 4-methyl-benzylidene camphor (4-MBC) and octocrylene (OC), to protozoan Tetrahymena thermophila were investigated in this study. The 24-h EC₅₀ values with 95% confidence intervals for BP-3 and 4-MBC were 7.544 (6.561–8.675) mg L⁻¹ and 5.125 (4.874–5.388) mg L⁻¹, respectively. EHMC and OC did not inhibit the growth of T. thermophila after 24 h exposure at the tested concentrations. The results of cell viability assays with propidium iodide (PI) staining were consistent with that of the growth inhibition tests. As for BP-3 and 4-MBC, the relatively higher concentrations, i.e. of 10.0 and 15.0 mg L⁻¹, could lead to the cell membranes impairment after 4 h exposure. With the increase of the exposure time to 6 h, their adverse effects on cell viability of T. thermophila were observed at the relatively lower concentration groups (1.0 mg L⁻¹ and 5.0 mg L⁻¹). In addition, it is noticeable that at environmentally relevant concentration (1.0 μg L⁻¹), BP-3 and 4-MBC could lead to the significant increase of catalase (CAT) activities of the T. thermophila cells. Especially for the BP-3, the oxidative injuries were further confirmed by the reduction of glutathione (GSH) content. It is imperative to further investigate the additive action of UV filters and seek other sensitive endpoint, especially at environmentally relevant concentration.