Myrothecium verrucaria NF-08产漆酶条件的响应面法优化及粗酶染料脱色研究

以半知菌属疣孢漆斑菌Myrothecium verrucaria NF-08为供试菌,采用响应面方法优化菌株产漆酶条件,并以其漆酶粗酶进行3种结构类型6种染料的脱色试验,考察了粗酶浓度、温度和p H值对脱色率的影响.两水平析因设计、中心组合设计和验证试验结果表明:葡萄糖浓度为15.4 g·L~(-1)、蛋白胨浓度为43.3 g·L~(-1)和培养基初始p H值为6.68时,漆酶活性达到最大值(25.58±1.60)U·m L~(-1),是单因素试验(16.82 U·m L~(-1))的1.52倍,提高了52.1%.以上述发酵条件获得的M.verrucaria NF-08漆酶粗酶对3种结构类型染料均有脱色效果,排序为偶氮类优于芳甲烷类优于蒽醌类.脱色率随粗酶浓度增大(2~8 U·m L~(-1))、反应温度升高(15~35℃)而上升,随p H值上升(4~10)而下降.粗酶浓度为5 U·m L~(-1),反应温度为25℃,p H值为4时,偶氮类染料铬黑T和芳甲烷类染料碱性品红即可几乎完全脱色,脱色率分别为99.2%和94.6%.     

速生树种竹柳对复合污染土壤中镉和锌的吸收、积累与生理响应特性

为探讨竹柳在重金属复合污染(镉、锌)土壤中生长的生理变化及重金属在植株内的转移情况,以竹柳(Salix sp.)三号及竹柳五号为试验材料,以重金属复合污染的水稻土为供试土壤,通过盆栽试验研究了重金属对竹柳生理指标、重金属吸收与积累量、富集系数(BCF)和转运系数(TF)的影响.结果表明:竹柳各器官重金属含量和转移情况不同,其中,叶片中Cd、Zn含量最高,分别达61.4和1940 mg·kg~(-1);竹柳三号对Zn的转运能力大于竹柳五号,而竹柳五号的净光合速率更高;生长100 d后,重金属在竹柳叶片中的积累量和转移系数均高于其他部位,竹柳吸收的重金属主要分布在叶片中,可通过集中处理叶片达到去除土壤中重金属污染的目的.     

汶川地震中远场深覆盖土层动力反应分析

对上海余山地震台在汶川地震中测得的基岩波地震记录进行频谱特性分析,然后以同济大学教学科研综合楼场地土层为研究对象,应用水平成层土层地震反应的一维有限元方法,分析该深覆盖场地在这一实测地震记录作用下的动力反应,并将其与同济大学强震监测台在汶川地震中获得的地表波记录进行对比.结果表明:汶川地震的远场地震动记录中,基岩波的长...     

破碎带对地下坑道动力响应影响的数值分析

为了研究破碎带的耗能作用及其对坑道结构动力响应的影响,根据某工程实例,建立了地冲击波作用下类岩体中坑道结构响应的计算模型,利用LS-DYNA3D有限元软件,分别计算了无破碎带和存在破碎带情况下坑道结构的动力响应。计算结果的对比分析表明,顶部破碎带使结构的加速度和位移等运动参数有不同程度的降低,但对结构所受到的应力影响不大;与之相反,侧部破碎带的存在使结构的加速度和位移等运动参数明显地增大。与顶部存在破碎带情况相比,侧部存在破碎带情况下坑道结构运动参数的峰值更大,对坑道结构安全更为不利。     

二维相关地震动作用下结构的随机反应

假定两水平方向的地震动为平稳随机过程,基于地震动主轴模型的假设,讨论了二维地震动分量的空间相关性,在单分量平稳模型的基础上给出了二维水平地震动随机模型的相关函数矩阵。该相关函数模型可以为二维地震下结构随机反应分析提供地震动输入。利用状态空间方法和随机振动理论建立了线性多自由度体系在二维地震激励下的随机反应分析方法,导出了结构的位移反应相关函数和速度反应相关函数,可在时域内直接计算结构的二维随机地震反应的统计特征。最后,通过一个二层框架结构计算实例,说明了这种方法的运用,讨论了地震动强度以及地震动主轴方位角对结构反应的影响。该方法可以为结构抗震可靠性评估提供基础。     

Monitoring aromatic hydrocarbon biodegradation by functional marker genes

The development of biological treatment technologies for contaminated environments requires tools for obtaining direct information about the biodegradation of specific contaminants. The potential of functional gene array analysis to monitor changes in the amount of functional marker genes as indicators of contaminant biodegradation was investigated. A prototype functional gene array was developed for targeting key functions in the biodegradation of naphthalene, toluene and xylenes. Internal standard probe based normalization was introduced to facilitate comparison across multiple samples. Coupled with one-colour hybridization, the signal normalization improved the consistency among replicate hybridizations resulting in better discrimination for the differences in the amount of target DNA. During the naphthalene biodegradation in a PAH-contaminated soil slurry microcosm, the normalized hybridization signals in naphthalene catabolic gene probes were in good agreement with the amount of naphthalene-degradation genes and the production of 14CO2. Gene arrays provide efficient means for monitoring of contaminant biodegradation in the environment.     

Causes of change in nitrophytic and oligotrophic lichen species in a Mediterranean climate: impact of land cover and atmospheric pollutants

With the aim of determining the main drivers of changes in nitrophytic and oligotrophic macro-lichen communities in an industrial region with a Mediterranean climate, we considered both land-cover types and atmospheric pollutants. We determined the relation between the abundance of nitrophytic and oligotrophic species with environmental factors considering the distance of influence of land-cover types. The results showed that oligotrophic species decreased in the proximity of artificial areas, barren land and agricultural areas, associated with higher concentrations of NO₂ and Zn, and Ti, probably dust of industrial and agricultural origin. Nitrophytic species were positively related to all the mentioned land-cover types, and with higher concentrations of Fe and N. Magnesium, probably from ocean aerosols, was negatively related to oligotrophic species and positively to nitrophytic.     

Modification of the biochemical pathways of plants induced by ozone: what are the varied routes to change?

When plants are observed under a low dose of ozone, some physiological and metabolic shifts occur. Barring extreme injury such as tissue damage or stomata closure, most of these disruptive changes are likely to have been initiated at the level of gene expression. The belief is oxidative products formed in ozone exposed leaves, e.g. hydrogen peroxide, are responsible for much of the biochemical adjustments. The first line of defense is a range of antioxidants, such as ascorbate and glutathione, but if this defense is overwhelmed, subsequent actions occur, similar to systemic acquired resistance or general wounding. Yet there are seemingly unrelated metabolic responses which are also triggered, such as early senescence. We discuss here the current understanding of gene control and signal transduction/control in order to increase our comprehension of how ozone alters the basic metabolism of plants and how plants counteract or cope with ozone.     

Collaborative emergency management: better community organising, better public preparedness and response

Community coordination requires communication and planning of precautions to take when faced with a severe threat of disaster. The unique case of the four Florida hurricanes of 2004--Charley, Frances, Ivan, and Jeanne--is used here to assess community responses to repeated threats of hurricanes. The paper examines how effectiveness in coordinating community disaster response efforts affects future public preparedness. The findings suggest that pre-season planning, open communication between emergency managers and elected officials, and the use of technology all had a significant impact on community responses. The repeated threat scenario indicates that emergency managers must work vigilantly to keep residents informed of the seriousness of a situation. The study describes how emergency managers in Florida countered public complacency during four hurricanes in six weeks. The strategies identified as useful by public managers in the context of hurricanes are applicable to other natural and man-made disasters.     

覆冰输电塔-线体系风致动力响应分析

依据流体诱发振动原理,结合已有覆冰计算模型,考虑降雨的分类,模拟了输电塔-线体系不同高度导线的覆冰和风荷载。以东北地区某输电塔为例,对覆冰输电塔-线体系在稳定风速激励下的动力响应进行分析,并与不考虑塔线耦联的导线舞动分析结果及覆冰塔线拟静力的分析结果进行对比,对塔位移、输电线位移、钢材应力进行了分析。研究结果表明:塔-线耦联体系对覆冰导线风振有很大影响,覆冰输电塔抗风设计安全度需要进一步提高。