乙虫腈悬浮剂对新出房意大利蜜蜂的毒性研究

乙虫腈是在我国登记并广泛使用的氟虫腈替代药剂,其商品化制剂为拜尔公司研发的100 g·L~(-1)悬浮剂,目前关于乙虫腈制剂对非靶标生物的毒性研究少有报道。为探究乙虫腈制剂对蜜蜂的毒性作用,采用人工饲喂法分别测定了100 g·L~(-1)乙虫腈悬浮剂对新出房意大利蜜蜂的48 h急性和14 d慢性毒性作用。结果显示:乙虫腈悬浮剂对新出房意大利蜜蜂的48 hLC_(50)值为0.21 mg·L~(-1)(95%置信限0.16～0.26 mg·L~(-1)),相应的48 h-LD50为5.0×10~(-3)μg a.i.·蜂~(-1)(95%置信限1.0×10~(-3)～1.0×10~(-2)μg a.i.·蜂~(-1));在亚致死剂量下,蜜蜂死亡数量随暴露时间的延长而增多,且100 g·L~(-1)乙虫腈悬浮剂会引起蜜蜂显著的氧化应激反应,诱导抗氧化酶SOD和CAT活力、脂质过氧化产物MDA含量、解毒酶GST活力和底物GSH含量的显著变化。研究表明,乙虫腈悬浮剂对意大利蜜蜂毒性较高,即使在亚致死剂量下亦存在显著的影响。田间施用应防止药剂的漂移污染,并避免在作物花期施用;新出房意大利蜜蜂对药剂敏感,未来可探索其作为风险指示生物应用的可能。     

Emission reduction and profit-neutral permit allocations

The present paper addresses two policy objectives: to implement a market for pollution permits and to make regulation acceptable for businesses. Profit-neutral permit allocations are defined as the number of permits that the regulator should give for free so that post-regulation profits (i.e. a firm's profits in the products market plus the value of the allowances granted for free) are equal to pre-regulation profits. The proposed model is developed by assuming that firms use polluting technologies and compete “à la Cournot”. The paper demonstrates that a low number of free allowances is sufficient to meet these two goals. Moreover, the regulator can fully offset losses, even when the reduction in emissions is high, provided that the sectors concerned are not monopolies, both for isoelastic and linear demand functions.     

Tell the truth or not? The montero mechanism for emissions control at work

We experimentally test the truth-telling mechanism proposed by Montero (2008) for eliciting firms' abatement costs. We compare this mechanism with two well-known alternative allocation mechanisms, free and costly allocation of permits at the Pigouvian price. Controlling for the number of firms and the firms' maximal emissions, we find that, in line with the theoretical predictions, firms over-report their maximal emissions under free allocation of permits and under-report these under costly allocation of permits. Under Montero's mechanism, by contrast, firms almost always report their maximal emissions truthfully. However, in terms of efficiency, the difference between Montero's mechanism and costly allocation disappears with industries including more than one firm.     

Effects of EGR on performance and emissions of a diesel engine fuelled with balanites aegyptiaca/diesel blends

In this study, balanites Aegyptiaca (L.) Del biodiesel was blended in proportions of 10% and 20% on the volume basis with diesel fuel and tested in a single cylinder, VCR diesel engine under measured load conditions with varied EGR rates (0, 10 and 20%). The results showed that B10 and B20 blends shown a significant reduction rate in terms of NO_x emissions that were familiar with biodiesel blends. At peak load conditions, BTE increased slightly for test fuel blends compared with pure diesel fuel while the BSFC rate and EGT suffered from increasing and decreasing nature with respect to blending percentage. From the emissions point of view, with the increase in blends percentage, a significant reduction rate is observed in terms of CO and HC concentrations (up to 12.34 and 17.5%, respectively) while NOx emissions decreased at peak load conditions (up to 24.34%). HC and CO emissions decreased with increase in blends percentage. However, lower levels of NO_x and EGT (up to 21.37 and 8.47%, respectively) and the average increase in terms of BTE and BSFC (up to 2.83 and 2.9%, respectively) can be realised with B20 test fuel blend under 20% EGR rate.     

Experimental and CFD-DEM study of the dispersion and combustion of wheat starch and carbon-black particles during the standard 20L sphere test

The 20L sphere is one of the standard devices used for dust explosivity characterization. One concern about the effectiveness and reliability of this test is related to the particle size variation due to particles' agglomeration and de-agglomeration. These phenomena are related to the turbulent regime of the dust cloud during the dispersion. This variable must be considered since it determines the uncertainty level of the ignitability and severity parameters of dust combustion. In this context, this study describes the influence of the cloud turbulence on the dust segregation and fragmentation through a study combining both, experimental and computational approaches. The behavior of the gas-solid mixture evidenced with the standard rebound nozzle was compared with that observed with six new nozzle geometries. Thereafter, the time-variation of the Particle Size Distribution (PSD) within the 20L sphere was analyzed for two different powders: carbon-black and wheat starch. On the one hand, the turbulence levels and PSD variations were characterized by Particle Image Velocimetry (PIV) tests and granulometric analyses, respectively. On the other hand, a computational approach described the dispersion process with CFD-DEM simulations developed in STAR-CCM + v11.04.010. The simulation results established that the homogeneity assumption is not satisfied with the nozzles studied. Nonetheless, the particles segregation levels can be reduced using nozzles that generate a better dust distribution in the gas-solid injections. Subsequently, an additional first-approach CFD model was established to study the behavior of the combustion step for a starch/air mixture. This model considers the gas-phase reactions of the combustible gases that are produced from the devolatilization of wheat starch ($CO,C{H}_4,C_2{H}_4,C_2{H}_6,C_2{H}_2,$ and $H_2$) and allowed to establish the approximate fraction of the particle mass that devolatilizes, as well as to confirm that the modeling of the pyrolysis stage is essential for the correct prediction of the maximum rate of pressure rise.     

滤纸对青蒿丛生芽诱导及遗传转化的影响

研究了滤纸对青蒿丛生芽诱导及遗传转化的影响.结果表明,培养基上加铺一层滤纸能够明显促进青蒿丛生芽的诱导,丛生芽诱导率可达到97%.在高效丛生芽诱导体系的基础上,探讨了滤纸在根癌农杆菌介导的青蒿遗传转化中的应用.优化后的青蒿转化体系,抗性丛生芽诱导率可达到59.7%,其中,12.5%的抗性丛生芽能发育成抗性生根植株,生根植株PCR检测均为阳性.在部分PCR检测阳性植株中检测到了GUS的稳定表达.该优化的转化体系对青蒿素生物合成分子调控的研究具有重要意义.图5表1参26     

五爪金龙凋落叶腐解物的化感潜力研究

采用莴苣种子为受体,测试了五爪金龙凋落叶早期腐解物的化感作用,并与其鲜叶及凋落叶的化感潜力进行了比较分析。结果如下:凋落叶40d的腐解物、鲜叶及凋落叶的水提取液均具有较强的化感潜力,并表现出低促高抑的浓度效应,在FW0.1g﹒mL-1高质量浓度下的综合化感抑制强度为凋落叶未能加土壤的腐解物>鲜叶>凋落叶加土壤的腐解物>凋落叶,而在FW0.005g﹒mL-1低质量浓度下的综合化感促进作用是凋落叶>鲜叶>凋落叶加土壤的腐解物>凋落叶未加土壤的腐解物。鲜叶的抑制强度较凋落叶的大,表明叶片在衰老过程中抑制作用减弱。凋落叶未加土壤仅在空气微生物作用下的腐解物抑制作用最强,促进作用最小,而加入土壤后在土壤微生物作用下的腐解物的抑制作用却有所减弱,抑制强度介于鲜叶和凋落叶的之间,促进作用却小于鲜叶和凋落叶,说明凋落叶腐解过程中微生物的参与对化感作用产生了复杂的影响。     

土壤水分对沙棘成活率及抗逆生理特性的影响

为提高沙棘在滩涂区，半干旱区造林的成活率，对不同土壤水分下沙棘的生理学特性进行了分析。结果表明：（1）在不同土壤水分处理下，随土壤含水量降低及处理时间的延长，沙棘叶片脯氨酸（Pro)含量升高，POD活性增强，可溶性糖含量升高；水淹情况下，沙棘叶片Pro含量明显上升，POD活性增强，可溶性糖含量升高。（2）土壤含水量在15%-30%之间，沙棘苗木成活率最高（100%），生长最好；35%和40%处理下，沙棘苗木成活率较高（66.7%)，生长良好；5%，10%处理水平和水淹情况下，沙棘苗木成活率低（33.3%)，生长不良，（3）不同处理下，随土壤含水量的降低，沙棘叶片的相对电导度增大，沙棘受伤害的程度加剧，且淹水对沙棘的伤害最重。     

低磷胁迫下不同水稻品种根系吸收能力差异的生理与遗传本质

在低磷（1mg L-1)和适磷水平（10mg L^-1)下，分别以混合培养方式研究了粳稻京系17（Oryza sativa ssp.japonica)(JX17)和粘稻窄叶青8号（Oryza sativa ssp.indica)(ZYQ8)对磷营养的反应。结果表明，低磷胁迫下，JX17较ZYQ8吸收更多磷素，干物重也显著增加。相反，ZYQ8则受到更强的磷胁迫，吸磷量和干物重降低，主要是因为JX17具有较高的吸磷速度，单位根长吸磷量大，即根系高亲和力磷酸盐转运蛋白表达强，最大限度地吸收可能利用的磷素，从而改善植株营养状况，同时又有较多的磷被分配到根系，使根第，根表面积和根干重相应提高，进一步增加了其在低磷胁迫条件下的竞争磷营养的优势。图3表2参17     

入侵物种三裂叶蟛蜞菊挥发物的化感作用研究

对华南地区重要外来入侵物种三裂叶蟛蜞菊挥发物的化感作用进行了研究,以揭示该物种的入侵机制。采用水蒸气蒸馏法提取三裂叶蟛蜞菊地上部分的挥发油,提取率为2.58‰(质量比)。以稗草、三叶鬼针草和青葙3种杂草种子为供试对象,用种子萌发法测定了三裂叶蟛蜞菊挥发物的化感作用活性。结果表明:三裂叶蟛蜞菊的茎叶在密闭系统中产生的挥发性物质对稗草、三叶鬼针草和青葙的幼苗生长有显著抑制作用。稗草的根长、苗高和鲜质量分别比对照减少49.1%、35.0%和27.3%,三叶鬼针草的分别比对照减少18.8%、20.9%和28.6%,青葙的分别比对照减少62.9%、21.2%和50.0%。水蒸气蒸馏法所得的挥发油对稗草、三叶鬼针草和青葙的幼苗生长也有显著抑制作用,且抑制作用随着浓度的增大而增强。稗草的根长、苗高和鲜质量分别比对照减少88.2%、58.3%和45.5%,三叶鬼针草的根长、苗高和鲜质量分别比对照减少38.6%、34.5%和71.4%,青葙的根长、苗高和鲜质量分别比对照减少83.7%、51.5%和75.0%。结论:三裂叶蟛蜞挥发物具有化感作用,其作用机理值得深入研究。