Does anthracene affect microbial activities and organic matter decomposition? A comparative study in Pinus halepensis litters from Mediterranean coastal and inland areas

The widespread concern about pollution caused by Polycyclic Aromatic Hydrocarbons (PAHs) raises the question of how they affect soil microbial communities which are potentially involved in the transformation of these pollutants. Using microcosms, we describe the effect of anthracene, a model PAH, on microbial communities inhabiting a Pinus halepensis litter from both coastal (COS) and inland (INL) Mediterranean sites. The microcosms were incubated over 3 months (25 °C, 60% WHC) and the effects of anthracene on microbial activities of both litters were monitored. Different enzyme activities (laccase, cellulase, β-glucosidase and acid phosphatase) and microbial respiration were measured and variations in litter chemical composition over incubation were determined using ¹³C Nuclear Magnetic Resonance (NMR) from both sites. Our results show that lignocellulolytic enzymes increased markedly after a 3-month incubation in COS microcosms, especially in the presence of anthracene, whereas INL microcosms were not similarly affected. These results show that anthracene not only has no toxic effect on the microbial activities tested but actually enhances the lignocellulolytic activities of the fungal communities from coastal litters, demonstrating the detoxification potential and resistance of stressed Mediterranean coastal ecosystems.     

工业园区污水处理工艺的运行及完善探讨

本文对某工业园区的综合废水处理工艺开展了连续两年的运行调查,进行了其工艺完善及提标改造的技术探讨。研究表明,改造前该污水处理装置的出水COD、BOD5、总磷、SS均能达到排放标准,而唯独出水氨氮处理效果差,难以达到一级排放标准要求。通过增设预处理水解装置、生物氧化装置导流墙容积比改进、生物塘深度处理工艺完善后,能够保证出水氨氮的处理效果且能达到一级A排放标准。     

Attractancy evaluation of ceralure‐B1 isomer via field captures of mediterranean fruit flies (Diptera: Tephritidae)

Abstract

Commercially prepared ceralure‐B1, an attractant for the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), was evaluated in trapping tests by comparison with commercial ceralure and trimedlure. Jackson traps were used for medfly captures. The residual ceralure‐B1, ceralure, and trimedlure contents of aged dispensers were determined. The results of this study show that male medfly captures from traps with ceralure‐B1 were as high or higher than male medfly captures from traps with twice the quantity of ceralure or trimedlure. The use of regioselectively synthesized ceralure‐B1, enriched in B1, as a source of a more potent attractant than commercial ceralure and as a more persistent male medfly attractant than trimedlure is reported.     

Total and Leachable Heavy Metals in Muddy and Sandy Sediments of Egyptian Coast Along Mediterranean Sea

The assessment of marine pollution due to metals was made for surficial sediments sampled from 20 sites along Mediterranean coast of Egypt. The samples were dried, acid digested and analyzed for leachable and total heavy metal contents (Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, and Zn) by flame atomic absorption spectrophotometer (air–acetylene) with deuterium background correction. Evaluation of the heavy metals pollution status was carried out using enrichment factors (EFs), the effect range-low (ERL) and the effect range-median (ERM). The study showed high concentrations of Cd, Co, Pb, Ni and moderate concentrations of Cr, Cu and Mn were contaminated in the sediments of studied sites. The results of Spearman correlation, factor and cluster analysis of the heavy metals analyzed in the collected sediment were discussed. The main source of contamination is the offshore oil field and industrial wastes, which arise due to the ineffective and inefficient operation equipments, illegal discharge and lack of supervision and prosecution of offenders.     

Shipboard Measurements of Nitrogen Dioxide,Nitrous Acid,Nitric Acid and Ozone in the Eastern Mediterranean Sea

Measurements of nitrogen dioxide, nitrous and nitric acids as well as ozone were made using newly developed instrumentation onboard the research vessel Aegeon in the Aegean Sea between 25th–29th July 2000. Typical nitrogen dioxide concentrations observed aboard the boat were 4–6 ppb (v/v) with a broad maximum of 20–30 ppb (v/v). Ozone concentrations typically ranged between 40 and 80 ppb (v/v). Mixing ratios of both nitric and nitrous acids in the ambient air of the Aegean Sea were mainly below 50 ppt (v/v). The data also showed a number of short pollution episodes with rapid changes in the concentration of reactive nitrogen compounds [nitrogen dioxide maximum up to 164 ppb (v/v), nitric acid maximum up to 12 ppb (v/v), nitrous acid maximum up to 2.7 ppb (v/v)] and ozone [maximum up to 88 ppb (v/v)]. These episodes were correlated with pollution plumes originating from boats upwind, at short distance, from the R/V Aegeon. The measurements revealed the importance of nitrous and nitric acids for the transport of nitrogen to marine biota in busy ship lanes.     

An inhibitor of urease activity effectively reduces ammonia emissions from soil treated with urea under Mediterranean conditions

Urea is an important source of ammonia (NH₃) emissions to the atmosphere from agricultural soils. Abatement strategies are necessary in order to achieve NH₃ emission targets by reducing those emissions. In this context, a field experiment was carried out on a sunflower crop in spring 2006 with the aim of evaluating the effect of the N-(n-butyl) thiophosphoric triamide (NBPT) in the mitigation of volatilized NH₃ from a urea-fertilised soil. Ammonia emission was quantified, using the integrated horizontal flux (IHF) method, following application of urea with and without the urease inhibitor NBPT. Urea and a mixture of urea and NBPT (0.14%, w/w) were surface-applied at a rate of 170 kg N ha⁻¹ to circular plots (diameter 40 m). The soil was irrigated with 10 mm of water just after the application of urea to dissolve and incorporate it into the upper layer of soil. Over the duration of the measurement period (36 days) three peaks of NH₃ were observed. The first peak was associated with hydrolysis of urea after irrigation and the others with the increase of ammonia in soil solution after changes in atmospheric variables such as wind speed and rainfall. The total NH₃ emission during the whole experiment was 17.3 ± 0.5 kg NH₃–N ha⁻¹ in the case of urea treated soils and 10.0 ± 2.2 kg NH₃–N ha⁻¹ where NBPT was included with the urea (10.1 and 5.9%, respectively, of the applied urea–N). The lower NH₃ emissions from plots fertilised with urea + NBPT, compared with urea alone, were associated with a reduction in urease activity during the first 9 days after inhibitor application. This reduction in enzymatic activity promoted a decrease in the exchangeable NH₄⁺ pool.     

How does agricultural intensification modulate changes in plant community composition?

Agricultural intensification, at local and landscape scales, has caused a decrease in plant diversity and changes in species composition in cereal fields. To better understand the role of landscape complexity and farming systems in shaping plant assemblages, it is of interest to focus on functional traits rather than on floristic composition, which may help to highlight trends in vegetation patterns. We analysed the relative abundance of various functional attributes (different life forms, growth forms, wind-pollinated species and wind-dispersed species) at three contrasted field positions (boundary, edge and centre) of 29 organic and 29 conventional cereal fields distributed in 15 agrarian localities of NE Spain. Agricultural intensification affected the biological attributes of the vegetation in dryland Mediterranean cereal fields; local factors (farming system and position) had a more prominent role in affecting plant functional composition than the surrounding landscape. Local factors were important for life form distribution, growth form and pollination type, whereas landscape complexity mainly affected the proportion of wind-dispersed species. Therefore, depending on the objective of the study, it is important to select functional attributes sensitive to the different scales of agricultural intensification, especially because landscape complexity and land-use intensity are commonly related.     

On the tolerance of charophytes to high-nitrate concentrations

Currently a debate exists about whether the reduced growth of macrophytes with increased nitrogen loading in shallow ecosystems is determined by ecological or physiological factors. To discover whether nitrate in the water is detrimental per se to charophytes, we subjected Chara hispida and Chara vulgaris specimens, collected from two habitats greatly differing in nitrate concentrations (1.5 and 10?mg NO₃-N/L, annual means), to a wide nitrate range (0.5–50?mg NO₃-N/L) in two experiments (with free-floating specimens using nitrate as the sole N source, and with planted specimens, with other N sources in sediment). Charophytes grew both unplanted and planted in all treatments, and growth reductions occurred at the highest concentration in all cases. Some charophyte responses when faced with nitrate increases were different depending on (i) the species and (ii) population origin. Under the most realistic situation, the growth of both planted C. vulgaris populations was higher than that of C. hispida populations. C. vulgaris specimens from the nitrate-rich waterbody adapted best to the highest nitrate concentrations when they grew floating. Despite charophytes being vital and growing under high-nitrate concentrations in short-term laboratory experiments, such a situation in the environment may eventually not be sustainable, since ecological factors act in the field.     

Endocrine Disruptors in Mediterranean top marine predators

- DOI: http://dx.doi.org/10.1065/espr2006.01.018 Background, Aims and Scope Man-made Endocrine Disruptors (EDs) range across all continents and oceans. Some geographic areas are potentially more threatened than others: one of these is the Mediterranean Sea. Levels of some xenobiotics are much higher here than in other seas and oceans. In this paper we review the final results of a project in which the hypothesis that Mediterranean top predator species (such as large pelagic fish and marine mammals) are potentially at risk due to EDs was investigated. Methods In a four-year survey on the Mediterranean population of swordfish (Xiphias gladius), the potential toxicological effects of organochlorine compounds (OCs) on specimens of swordfish and tuna fish (Thunnus thynnus thynnus), caught in the spawning seasons from 1999 to 2002 in the Straits of Messina, Sicily (Italy), were investigated using vitellogenin (Vtg), Zona radiata proteins (Zrp), and cytochrome P4501A (CYP1A) activities (EROD, BPMO). Tissues (skin and blubber) were obtained from Stenella coeruleoalba, Tursiops truncatus, Delphinus delphis and Balaenoptera physalus from the western Ligurian Sea, between Corsica and the French-Italian coast, and Ionic Sea using biopsy darts launched with a crossbow. Benzo(á)pyrene monoxigenase (BPMO) activity was mesured in biopsies and cholrinated hydrocarbon levels were detected. Results and Discussion We illustrate the need to develop and apply sensitive methodological tools, such as biomarkers (Vitellogenin, Zona Radiata proteins and CYP1A activities) for evaluation of toxicological risk in Xiphias gladius and Thunnus thynnus thynnus), and nondestructive biomarkers (CYP1A activities and fibroblast cell culture in skin biopsy), for the hazard assessment of threatened marine mammals species (Stenella coeruleoalba, Tursiops truncatus, Delphinus delphis and Balaenoptera physalus) exposed to EDs. Conclusion The present research shows that: a) Vtg and Zrp can be used as diagnostic tools for fish stocks hazard assessment in the Mediterranean Sea; b) that CYP1A1 (BPMO) induction in cetaceans skin biopsy may be an early sign of exposure to EDs such as OCs and a potential alert for transgenerational effects. Recommendation and Outlook This research represents a warning signal of the potential reproductive alterations in marine top predators and suggest the need for continuous monitoring to avoid reductions in population and biodiversity in the Mediterranean Sea.