转基因毛白杨外源基因转移的环境风险分析

以8年生转Bt基因败育毛白杨雄株为实验材料,分离其体内、体外、根际土壤、蛀干桑天牛(Apripona germari Hope)排泄物中可培养的细菌和真菌,利用特异引物对获得的微生物进行转Bt基因败育毛白杨雄株外源基因PCR检测.实验发现,381株次细菌中有6株PCR检测阳性;307株次真菌中有4株PCR检测阳性.     

Trace element accumulation in woody plants of the Guadiamar Valley, SW Spain: a large-scale phytomanagement case study

Phytomanagement employs vegetation and soil amendments to reduce the environmental risk posed by contaminated sites. We investigated the distribution of trace elements in soils and woody plants from a large phytomanaged site, the Guadiamar Valley (SW Spain), 7 years after a mine spill, which contaminated the area in 1998. At spill-affected sites, topsoils (0-25 cm) had elevated concentrations of As (129 mg kg(-1)), Bi (1.64 mg kg(-1)), Cd (1.44 mg kg(-1)), Cu (115 mg kg(-1)), Pb (210 mg kg(-1)), Sb (13.8 mg kg(-1)), Tl (1.17 mg kg(-1)) and Zn (457 mg kg(-1)). Trace element concentrations in the studied species were, on average, within the normal ranges for higher plants. An exception was white poplar (Populus alba), which accumulated Cd and Zn in leaves up to 3 and 410 mg kg(-1) respectively. We discuss the results with regard to the phytomanagement of trace element contaminated sites.     

大兴安岭南部温带山杨天然次生林不同生长阶段生物量及碳储量

基于内蒙古赛罕乌拉森林生态系统定位研究站山杨（Populus davidiana Dode）天然次生林幼龄林、中龄林、近熟林、成熟林及过熟林生物量调查,探讨了不同龄组山杨天然次生林单株木、林分、林下植被和枯落物的生物量及群落碳储量的时空变化规律。结果表明：随林龄的增大,山杨天然次生林木和各器官生物量总体呈增加趋势,树干所占比例增加,中龄林增加尤为明显;林下植被层、枯落物层生物量随林龄增大呈增加趋势。群落总碳储量的空间分布序列是：乔木层〉枯落物层〉林下植被层。幼龄林、中龄林、近熟林、成熟林和过熟林群落的碳储量分别为27.146 6、53.545 1、60.889 8、77.915 8、79.135 3t.hm-2,乔木层碳储量分别为22.206 5、47.215 7、52.056 3、68.445 3、68.773 1 t.hm-2,枯落物层和林下植被层碳储量平均值分别为5.814 4、2.172 7 t.hm-2。乔木层、枯落物层和林下植被层碳储量占总量的平均率分别为86.05%、10.39%和3.57%。研究认为山杨天然次生林群落碳储量随林龄增加的变化规律明显,碳汇潜力巨大;中龄林为碳储量增长迅速期,且持续较长一段时间,是林分管理的关键阶段;自然稀疏有利于促进林木生长,林分碳储量并未随林分密度下降而减小。     

Regeneration of Native Trees in the Presence of Invasive Saltcedar in the Colorado River Delta, Mexico

Abstract:  Many riparian zones in the Sonoran Desert have been altered by elimination of the normal flood regime; such changes to the flow regime have contributed to the spread of saltcedar ( Tamarix ramosissma Ledeb.), an exotic, salt-tolerant shrub. It has been proposed that reestablishment of a natural flow regime on these rivers might permit passive restoration of native trees, without the need for aggressive saltcedar clearing programs. We tested this proposition in the Colorado River delta in Mexico, which has received a series of large-volume water releases from U.S. dams over the past 20 years. We mapped the vegetation of the delta riparian corridor through ground and aerial surveys (1999–2002) and satellite imagery (1992–2002) and related vegetation changes to river flood flows and fire events. Although saltcedar is still the dominant plant in the delta, native cottonwood (  Populus fremontii S. Wats.) and willow ( Salix gooddingii C. Ball) trees have regenerated multiple times because of frequent flood releases from U.S. dams since 1981. Tree populations are young and dynamic (ages 5–10 years). The primary cause of tree mortality between floods is fire. Biomass in the floodplain, as measured by the normalized difference vegetation index on satellite images, responds positively even to low-volume (but long-duration) flood events. Our results support the hypothesis that restoration of a pulse flood regime will regenerate native riparian vegetation despite the presence of a dominant invasive species, but fire management will be necessary to allow mature tree stands to develop.     

The response of Populus spp. to cadmium stress: Chemical,morphological and proteomics study

Poplar (Populus) species are seen as candidates for removing heavy metal contamination from polluted soil. A bottom-up multidisciplinary approach was utilized to compare the performances of clones 58-861 and Poli (Populus nigra) and A4A, a Populus nigra × Populus deltoides hybrid to Cd toxicity. Qualitative and quantitative differences in their tolerance to Cd exposure and the uptake, accumulation and translocation of Cd were noted following the hydroponic exposure of rooted cuttings to 20 μM CdSO₄ for either 48 h or 14 d. Cadmium was less toxic for the hybrid clone A4A as compared to Poli and 58-861. Cd uptake and root to shoot translocation were determined by AAS, and its compartmentation was analyzed using SEM/EDX. A comparative proteomic approach was utilized to identify changes in proteins expression according to dose and time of exposure. Toxicity to Cd mainly influenced proteins related to general defense, stress response and carbohydrate metabolism.     

干旱胁迫对青海杨不同种群的影响

通过对青海杨的两个分别来自极端干旱和湿润地区的种群进行不同水分处理,研究其在生物量的积累与分配、气体交换、脱落酸积累以及水分利用效率上的种群差异.结果表明:①不同种群在各种生长和形态指标上均表现出了显著性差异,例如株高(Ht)、基径(Bd)、总生物量(Tb)、总叶面积(La)和细根比(Ft).②不同种群在各种生理指标上也表现出了显著性差异,例如净光合速率(A)、蒸腾速率(E)、气孔导度(g)、水分利用效率(WUEi)和脱落酸(ABA)积累.③与来自湿润地区的种群相比,来自干旱地区的种群表现出了较小的生长和形态指标值以及较大的生理指标值.这些对水分可利用性的形态和生理适应性表明,不同种群在其幼苗早期生长和建立方面采用了不同的生存策略,来自湿润地区的杨树种群采用耗水策略从而抗旱性低,而来自干旱地区的杨树采用节水策略从而抗旱性高.这些对干旱的不同反应可以为不同气候地区选择相应的生态型提供一定的参考标准.表4参26     

塔里木河下游植被覆盖度变化与地下水质关系

植被覆盖度的变化是地球内部作用与外部作用的共同结果,但在短期内主要受人类活动的影响.人类对自然植被覆盖度的影响最常见的就是通过对水分和水质状况的改变而影响植被覆盖度的变化.本研究基于2002~2007年间塔里木河下游地下水化学特征变化的监测资料和植被覆盖度的适时监测,探讨了植被覆盖度与地下水质之间的关系.结果表明,植被覆盖度在沿河道方向上,从A断面的80%,下降到Ⅰ断面的不足10%;在垂直河道方向上表现为距离输水河道越近植被覆盖度越高的特点;地下水pH值也表现出沿河道方向从上段到下段,从距离河道近处向距离河道远处下降的变化趋势.回归分析发现,胡杨覆盖度随地下水pH值的升高而指数增加,柽柳覆盖度则随地下水pH值的增加而降低.胡杨和柽柳与地下水质的关系是造成胡杨主要分布于距离河道近处而柽柳则分布在远离河道处的因素之一.     

燕山典型流域两种造林树种生态系统碳储量及固碳潜力研究

选择燕山典型流域6个林龄序列的小叶杨（Populus simonii）和5个林龄序列的山杏（Prunus sibirica）主要造林树种为研究对象,利用时间替代空间样地测量法量化退牧还林后生物量碳储量、凋落物碳储量和土壤碳储量及生态系统碳储量的变化规律,同时以各组成碳库-林龄序列中的最大碳储量之和作为生态系统饱和碳储量,以未退牧的天然草地生态系统碳储量作为初始植被类型的碳储量,分析总结了退牧还林对生态系统碳储量和碳循环的影响。结果表明,退牧还林后生态系统的生物量碳储量、凋落物碳储量基本随退牧年限的增加而增加,土壤碳储量随退牧年限的增加呈现先减小后增加的趋势。在没有人为干扰的情况下,9、15、18、22及29 a生小叶杨林的生态系统碳储量分别为7147.45、7461.67、7509.895、8468.375及8247.85 g·m^-2,9、15、18、22及26 a生山杏林的生态系统碳储量分别为6695.44、6700.82、8011.86、8001.92及7981.92 g·m^-2;9、15、18、22、29及36 a生小叶杨林的生态系统固碳潜力分别为757.08、1071.3、1119.53、2078.01、1857.48及1312.21 g·m^-2,9、15、18、22及26 a生山杏林的生态系统固碳潜力分别为310.45、1621.49、1611.55、1591.55及757.08 g·m^-2。长期来看,研究区退牧还林对提高生态系统碳汇能力是可观的、积极的。研究结果对提高造林对碳汇影响的估测能力提供数据支持,也为政府参与国际全球气候变化的谈判提供一个很好的案例研究和科学根据。     

Volatiles from the bark of trembling aspen, Populus tremuloides Michx. (Salicaceae) disrupt secondary attraction by the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Scolytidae)

Summary. Coupled gas chromatographic-electroantennographic detection (GC-EAD) analysis of the Porapak Q-captured volatiles from the bark of trembling aspen, Populus tremuloides Michx., revealed four compounds that consistently elicited antennal responses by mountain pine beetles (MPBs), Dendroctonus ponderosae Hopkins. One of these, 1-hexanol, disrupted the capture of MPBs in multiple-funnel traps baited with the aggregation pheromones trans-verbenol and exo-brevicomin and the host kairomone myrcene, a blend of semiochemicals that mediates the secondary attraction response in which beetles mass attack and kill living pines. The other three EAD-active aspen bark volatiles, benzyl alcohol, benzaldehyde and nonanal, were inactive alone, but in binary and ternary combinations contributed to a disruptive effect in an additive and redundant manner when all four aspen bark volatiles were tested in all possible binary and ternary blends. The best ternary blend and the quarternary blend achieved ≥ 80% disruption. The quarternary blend enhanced the disruptive effect of the antiaggregation pheromone verbenone in traps, raising the disruptive effect to 98%, and also enhanced the inhibition of attack on attractant-baited lodgepole pines. This is the first demonstration of specific compounds from the bark of angiosperm trees that disrupt the secondary attraction response of sympatric coniferophagous bark beetles. The results support the hypothesis that such bark beetles are adapted to recognize and avoid non-host angiosperm trees by responding to a broad spectrum of volatiles that can act in various blends with equal effect. Received 27 October 1997; accepted 20 February 1998.     

Fungicidal Activity of Artemisia herba alba Asso (Asteraceae)

The antifungal activity of Artemisia herba alba was found to be associated with two major volatile compounds isolated from the fresh leaves of the plant. Carvone and piperitone were isolated and identified by GC/MS, GC/IR, and NMR spectroscopy. Antifungal activity was measured against Penicillium citrinum (ATCC 10499) and Mucora rouxii (ATCC 24905). The antifungal activity (IC₅₀) of the purified compounds was estimated to be 5 μ g/ml, 2 μ g/ml against Penicillium citrinum and 7 μ g/ml, 1.5 μ g/ml against Mucora rouxii carvone and piperitone, respectively.