甲型流感病毒H5N1的siRNA设计

选用了甲型流感病毒10个毒株(全序列)和一个2004年越南毒株(部分序列)，采用自己编写的计算机程序sRNAFinder，对每个毒株的6个RNA片段的6个编码序列进行了siRNA设计．结合系统发育重建方法，利用sR-NAFinder的共有序列分析功能，对得到的6101个siRNA分子进行了计算机筛选．结果表明，设计的siRNA的分子，针对np和pb1应该是最有效的，而针对ha和na的siRNA分子设计，需要疾病流行期毒株的最新测序数据；从ns1的数据不能获得很保守的、可作为预防治疗药物的siRNA分子．图1表4参16     

A／A／O微曝氧化沟处理城市污水的工程设计

以某日处理量为80000m^3城市污水处理厂为例，介绍了A／A／O微曝氧化沟工艺的设计思路及参数选择，能够满足设计要求，且可信度高，有实用参考价值。     

Endocrine disrupter nonylphenol and bisphenol A contamination in Okinawa and Ishigaki Islands, Japan--within coral reefs and adjacent river mouths

Certain chemicals possess the potential to modulate endocrine systems, and thereby interfere with reproduction and developmental processes in the wild. We analyzed endocrine disrupters nonylphenol (NP) and bisphenol A (BPA) levels at various sites in Okinawa and Ishigaki Islands, Japan. River-water samples showed undetectable to low concentrations of NP and BPA at most of the sites investigated. However, an appreciable amount of BPA was detected in sediments at one coral reef site. In addition, significant numbers of river sediment samples showed appreciable amounts of NP and BPA. Most of the sampling sites for this study are located within a distance of 1 km from the coral reefs, which are under influence of river-waters to a variable extent. Therefore, influence of endocrine disrupters may have already begun on adjacent coral reefs. Both endocrine disrupters were positively correlated with human population densities, but not with the contents of red soil generated by farm land reformation. Therefore, it is concluded that NP and BPA pollution is a consequence of human waste discharge, both domestic and industrial, and not by agricultural activities.     

Furfural residues from straw become complex fertilizer by addition method

ＩｎｔｒｏｄｕｃｔｉｏｎＦｕｒｆｕｒａｌｉｓａｋｉｎｄｏｆｉｍｐｏｒｔａｎｔｒａｗｍａｔｅｒｉａｌｉｎｃｈｅｍｉｃａｌｉｎｄｕｓｔｒｙ.Ｗｉｔｈｔｈｅｇｒｏｗｔｈｏｆｉｔｓｕｓｅ,ｉｔｓｐｒｏｄｕｃｔｉｏｎｉｓｉｎｃｒｅａｓｉｎｇａｎｎｕａｌｌｙ.Ｂｕ?..     

A/O接触氧化法处理城市生活污水的工程实践

介绍了A O工艺 (内置填料 )处理城市生活污水的工程实例。监测数据表明 :该工艺成熟可靠、出水能够达到排放标准 ,是处理城市生活污水较理想的工艺选择     

A/O法处理炼化废水的运行实践

介绍了炼化废水的特点及以A/O法为核心的污水处理设施的运行状况,探讨生产中存在的问题,并进行相应的改造,通过优化运行,使出水达标排放。     

Sequential fractionation of reactive phosphorus in the sediment of a shallow eutrophic lake——Donghu Lake, China

ＩｎｔｒｏｄｕｃｔｉｏｎＳｅｄｉｍｅｎｔｐｈｏｓｐｈｏｒｕｓｈａｓｂｅｅｎｔｈｅｆｏｃｕｓｏｆａｎｕｍｂｅｒｏｆｓｔｕｄｉｅｓｄｕｅｔｏｉｔｓｒｏｌｅｉｎｔｈｅｅｕｔｒｏｐｈｉｃａｔｉｏｎｏｆｌａｋｅｓ.Ｐｈｏｓｐｈｏｒｕｓｆｒａｃｔｉｏｎｓｉｎｓｅ?..     

阿拉善地区生物多样性与自然保护区规划

由于特殊的自然地理条件和独特的地形地貌类型，阿拉善地区具有丰富的珍稀物种资源和多样的生态系统。本文依据保护生物多样性的要求，对该区重点保护区做了具体规划。     

Isolating fetal nucleated red blood cells from maternal blood: The baylor experience—1995

In our previous work we have isolated fetal cells from maternal blood and used fluorescent in situ hybridization (FISH) for chromosome-specific probes to detect aneuploidy. Current efforts in the Baylor College of Medicine programme are focusing on obtaining consistency in flow-sorting methodology and on determining sensitivity and specificity. To this end, systematic evaluation of five glycophorin A (gly A) antibodies all produced agglutination, leading us to abandon the use of gly A antibodies for positive selection of fetal cells. Conversely, we have found LDS-751 to be useful for nuclear selection. CD45 negative selection can best be accomplished by the use of flasks coated with goat antibodies against mouse antibodies. Positive selection by flow sorting for either CD71⁺ cells or gamma-globin-positive cells seems to be successful. Using these two approaches, we have recently detected male (fetal) cells in pregnancies in which the fetus was 46, XY in 10 of 18 and in 12 of 14 cases, respectively.