铅暴露U251细胞差异表达基因及相关通路(The Interrelated Pathways in Brain Human U251 Glioma Cells by Lead Exposure)

为探讨铅对体外培养的人神经胶质瘤U251细胞(human U251glioma cells,U251)暴露后基因表达的变化以及相关基因通路,选用乙酸铅暴露U251细胞.细胞在乙酸铅中暴露8h和24h后提取RNA,使用cDNA芯片分析基因表达情况,芯片扫描结果经归一化处理,设定Ratio值<0.5或≥2.0为表达有差异基因.结果表明,铅暴露U251细胞导致2840条基因差异表达,使用KEGG和BioCarta数据库分析代表性基因网络.结果发现,铅暴露U251细胞导致大量基因差异表达,涉及多个代谢及信号通路,与神经组织相关的主要信号通路有Ca2+信号通路、Jak-STAT信号通路、MAPK信号通路、Wnt信号通路等,还涉及配体-受体、细胞因子相互作用等.这些通路相互联结,构成复杂的网络系统,调控细胞的生物学功能.     

Depth profile of ²³⁶U/²³⁸U in soil samples in La Palma, Canary Islands

The vertical distribution of the ²³⁶U/²³⁸U isotopic ratio was investigated in soil samples from three different locations on La Palma (one of the seven Canary Islands, Spain). Additionally the ²⁴⁰Pu/²³⁹Pu atomic ratio, as it is a well establish tool for the source identification, was determined. The radiochemical procedure consisted of a U separation step by extraction chromatography using UTEVA^® Resin (Eichrom Technologies, Inc.). Afterwards Pu was separated from Th and Np by anion exchange using Dowex 1x2 (Dow Chemical Co.). Furthermore a new chemical procedure with tandem columns to separate Pu and U from the matrix was tested. For the determination of the uranium and plutonium isotopes by alpha spectrometry thin sources were prepared by microprecipitation techniques. Additionally these fractions separated from the soil samples were measured by Accelerator Mass Spectrometry (AMS) to get information on the isotopic ratios ²³⁶U/²³⁸U, ²⁴⁰Pu/²³⁹Pu and ²³⁶U/²³⁹Pu, respectively. The ²³⁶U concentrations [atoms/g] in each surface layer (∼2 cm) were surprisingly high compared to deeper layers where values around two orders of magnitude smaller were found. Since the isotopic ratio ²⁴⁰Pu/²³⁹Pu indicated a global fallout signature we assume the same origin as the probable source for ²³⁶U. Our measured ²³⁶U/²³⁹Pu value of around 0.2 is within the expected range for this contamination source.     

Spatial distribution of uranium and thorium in the surface soil around proposed uranium mining site at Lambapur and its vertical profile in the Nagarjuna Sagar Dam

The understanding and evaluation of the possible interactions of various naturally occurring radionuclides in the world's third largest man-made dam, Nagarjuna Sagar located in Andhra Pradesh, India and built on river Krishna assumed significance with the finding of uranium deposits in locations near the dam. For the present work, surface soil samples from the mineralized area of Lambapur, Mallapuram, Peddagattu and sediment core samples from the Nagarjuna Sagar dam were analyzed for naturally occurring radionuclides namely uranium and thorium using gamma spectrometric technique. Also toxic elements lead and chromium were analysed by the Energy Dispersive X-ray Fluorescence Spectrometer (EDXRF) technique. Surface soil samples show a variation from 25 to 291 Bq/kg (2.02–23.5 mg/kg) for ²³⁸U and 32–311 Bq/kg (7.9–76.9 mg/kg) for ²³²Th. U/Th concentration ratio in surface soil samples ranged from 0.19 to 0.31 and was found comparable with the nation wise average of 0.26. The study of sediment core samples reflected higher U/Th concentration ratio of 0.30–0.33 in the bottom section of the core as compared to 0.22–0.25 in the upper section. The concentration ratio in the upper section of the core was similar to the ratio 0.23 found in the western Deccan Basalt region through which the river originates. A higher concentration of lead and chromium was observed in the upper section of the core compared to bottom section indicating the impact of river input on the geochemical character of dam sediment.     

美国环保产业发展政策对我国的启示

当今环保产业作为新兴战略产业在世界范围内迅速成长。美国作为全球环保产业的领头羊,其环保产业的发展离不开政府行之有效的发展政策。美国政府主要通过法律法规、税收、贷款、债券、基金、放宽出口管制、以外交促贸易等政策措施,促进了美国环保产业的技术创新、投融资及国际贸易。借鉴美国政策经验,我国应在环保产业管制政策、技术创新政策、财税政策、国际贸易政策上做出相应改进,以促进我国环保产业积极健康发展。     

CUMULATIVE IMPACTS OF LANDUSE ON WATER QUALITY IN A SOUTHERN APPALACHIAN WATERSHED1

ABSTRACT: Water quality variables were sampled over 109 weeks along Coweeta Creek, a fifth-order stream located in the Appalachian mountains of western North Carolina. The purpose of this study was to observe any changes in water quality, over a range of flow conditions, with concomitant downstream changes in the mix of landuses. Variables sampled include pH, HCO₃^2?, conductivity, NO₃^?_?-N, NH₄⁺-N, PO₄^3?-P, C1^?-, Na, K, Ca²⁺, Mg²⁺, SO₄^2?, 5iO₂, turbidity, temperature, dissolved oxygen, total and fecal coliform, and focal streptococcus. Landcover/landuse was interpreted from 1:20,000 aerial photographs and entered in a GIS, along with information on total and paved road length, building location and density, catchment boundaries, hydrography, and slope. Linear regressions were performed to relate basin and near-stream landscape variables to water quality. Consistent, cumulative, downstream changes in water quality variables were observed along Coweeta Creek, concomitant with downstream, human-caused changes in landuse. Furthermore, larger downstream changes in water quality variables were observed during stormflow when compared to baseflow, suggesting cumulative impacts due to landscape alteration under study conditions were much greater during storm events. Although most water quality regulations, legislation, and sampling are promulgated for baseflow conditions, this work indicates they should also consider the cumulative impacts of physical, chemical, and biological water quality during stormflow.     

Community opinion concerning airport noise-abatement alternatives

In the present study we examined (1) community opinion of airport noise-abating alternatives and (2) the influence of perceived control on attitudes concerning noise-abatement policies and annoyance toward aircraft noise. We proposed that residents who perceive themselves as having little control over aircraft noise exposure would express higher levels of annoyance than residents who believe they have some degree of control. Furthermore, residents with low levels of perceived control would be less favorable toward proposed noise-abatement alternatives. Survey results collected from 239 households within the 65 CNEL contour of a community airport in Southern California support these hypotheses. Findings indicate strong public support for noise abatement and strong opposition to land-use alternatives. In addition, residents' perceptions of control in the airport planning and management process are significantly related to their annoyance with noise and their opinions about noise management policies.     

Factors influencing voting by the united states congress on the national energy act

A myriad of factors, both economic and political, influenced the voting by members of the United States Congress on the 1978 National Energy Act. Determinant factors considered in our analysis included the percentage of residents in the Congressman's district residing in the central city or on farms; the percentage of residents who belong to labor unions; the average level of education of the residents; oil, coal, and natural gas production in the state relative to total domestic production. The results, determined by means of logit analysis, indicate that the impact on low-income energy consumers, the effect on overall employment, the impact on farmers, and the benefits to energy interests, as well as ideology and the subjective perception that the need exists to do something about the energy situation in the United States, were all important explanatory factors.The author is an economist with the United States Department of Energy. The views expressed are those of the author and do not necessarily represent the policies of the Department of Energy or the views of other Department of Energy staff members.     

Perceptions of the security and attractiveness of urban parking lots

This study concerns design features of urban parking lots which can increase the perception of such areas as both safe and attractive. College students evaluated the attractiveness and perceived security associated with 180 scenes of parking lots adjacent to commercial and multi-family residential structures in Atlanta and Athens, Georgia, U.S.A. High intergroup correlations (r > 0·90) indicated the reliability of evaluations of both perceived features. Regressions of physical features on perceived security ratings and attractiveness ratings yielded highly predictive models (R² = 0·76 and 0·80, respectively). For both sets of ratings a Maintenance and Design factor accounted for the most variance. Although attractiveness ratings were higher as the total amount of vegetation increased in a scene, security was in general rated higher only when vegetation was well maintained and appeared to be installed as part of a landscape design. Attractiveness was generally higher for multi-family residential scenes than for commercial scenes. Perceived security was higher for frontal views of structures, especially when entrances were proximate to the viewer. Research implications are discussed in terms of the impact of natural features in urban areas for increasing attractiveness and reducing fear of crime.     

Small towns in the united states: a perspective

Small towns are an important part of the rural settlement fabric in the United States, but there is very little substantive research into their capacity to survive or their intrinsic importance. However, recently there has been increasing interest shown by social scientists in these problems. This paper looks at features of this research from the point of view of the population and economic structure of small towns. It concludes by arguing that the problems of small towns are not indigenous to the towns, and calls for a comprehensive national urban policy.